A5039754223- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Metabolomics and Mass Spectrometry Studies
- Dementia and Cognitive Impairment Research
- Mass Spectrometry Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
- Neurological Disorders and Treatments
- Extracellular vesicles in disease
- Acute Ischemic Stroke Management
- Atrial Fibrillation Management and Outcomes
- Diet and metabolism studies
- Cerebrovascular and Carotid Artery Diseases
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Immune cells in cancer
- Tryptophan and brain disorders
- Heart Rate Variability and Autonomic Control
- Barrier Structure and Function Studies
- Parkinson's Disease Mechanisms and Treatments
- Analytical Chemistry and Chromatography
- Hearing, Cochlea, Tinnitus, Genetics
- S100 Proteins and Annexins
- Biochemical effects in animals
Western University
2016-2025
Western University of Health Sciences
2024
University of Cape Town
2024
University of Ottawa
2007-2021
London Health Sciences Centre
2007-2020
Transnational Press London
2020
Boston Medical Center
2020
Northwestern University
2019
Canadian Institutes of Health Research
2017
Northeastern University
2014
Abstract For decades lysophosphatidylcholine (LPC, lysolecithin) has been used to induce demyelination, without a clear understanding of its mechanisms. LPC is an endogenous lysophospholipid so it may cause demyelination in certain diseases. We investigated whether known receptor systems, inflammation or nonspecific lipid disruption mediates LPC‐demyelination mice. found that nonspecifically disrupted myelin lipids. integrated into cellular membranes and rapidly induced cell membrane...
<h3>Objective</h3> To compare the risk of 1-year ischemic stroke recurrence between atrial fibrillation (AF) diagnosed after (AFDAS) and sinus rhythm (SR) investigate whether underlying heart disease is as frequent in AFDAS it AF known before (KAF). <h3>Methods</h3> In this retrospective cohort study, we included all patients admitted to institutions participating Ontario Stroke Registry from July 1, 2003, March 31, 2013. Based on assessed during admission, classified AFDAS, KAF, or SR. We...
Mild cognitive impairment (MCI) is a prodromal stage of dementia. Understanding the mechanistic changes from healthy aging to MCI critical for comprehending disease progression and enabling preventative intervention.
Alzheimer's disease (AD) is a progressive neurodegenerative that accounts for two-thirds of all dementia cases, and age the strongest risk factor. In addition to amyloid hypothesis, lipid dysregulation now recognized as core component AD pathology. Gangliosides are class membrane lipids glycosphingolipid family enriched in central nervous system (CNS). Ganglioside has been implicated various diseases, including AD, but spatial distribution ganglioside with respect amyloid-beta (Aβ)...
Gangliosides, a member of the glycosphingolipid family, are heterogeneously expressed in biological membranes and particularly enriched within central nervous system. Gangliosides consist mono- or poly-sialylated oligosaccharide chains variable lengths attached to ceramide unit found be intimately involved brain disease development. The purpose this study is examine spatial profile ganglioside species using matrix-assisted laser desorption/ionization (MALDI) imaging (IMS) following middle...
The brain's response to acute injury is characterized by increased permeability of the blood-brain barrier (BBB) and pro-inflammatory microglia signaling, both which have been linked poor cognitive outcomes neurological disease. damaged BBB has leakiness, allowing serum proteins like fibrinogen into brain, interacts with local cells in a deleterious manner. At same time, injury, demonstrate NLRP3 inflammasome activity heightened release cytokines. relationship between uptake microglial...
In the elderly, cerebral ischemia (CI) occurs in presence of high levels amyloid. Neuroinflammation plays a critical role pathophysiology Alzheimer's disease and CI. This study examined infarct size, neuroinflammation, cognitive deficits over time rat models CI.beta-amyloid toxicity was modeled using bilateral intracerebroventricular injections beta-amyloid 25 to 35 peptides. CI unilateral potent vasoconstrictor, endothelin-1, into striatum.Infarct volumes were higher amyloid compared with...
Perturbation of lipid second messenger networks is associated with the impairment synaptic function in Alzheimer disease. Underlying molecular mechanisms are unclear. Here, we used an unbiased lipidomic approach to profile alkylacylglycerophosphocholine messengers diseased tissue. We found that specific isoforms defined by a palmitic acid (16:0) at sn -1 position, namely 1-O-hexadecyl-2-acetyl- -glycero-3-phosphocholine (C16:0 PAF) and 1-O-hexadecyl- lyso -PAF), were elevated temporal cortex...
This article offers insight into post-stroke rehabilitation. Stroke is a major public health issue with high mortality and morbidity rates, both nationally internationally. Recent medical advances resulted in improvements but the disabling long-term effects of stroke are still prevalent. Post-stroke rehabilitation improves patient outcomes by decreasing chance developing secondary complications maximising patient's independence despite their impairments. delivered an interdisciplinary team...
Metabolic syndrome, the development of which is associated with high-caloric Western diet (HCD) intake, represent a risk factor for mild cognitive impairment (MCI) and dementia including Alzheimer's disease (AD) later in life. This study aimed to investigate effect diet-induced metabolic disturbances on white matter neuroinflammation function transgenic (TG) Fischer 344 rat carrying human β-amyloid precursor protein (APP) gene Swedish Indiana mutations (APP21 TG), model pre-AD MCI.TG...
Abstract Chronic microglia activation post-stroke is associated with worse neurological and cognitive outcomes. However, measurement of in vivo currently limited. Plasma derived extracellular vesicles (EVs) are cell-specific indicators that may allow for non-invasive phenotype. The aim this study was to identify activation-state specific EVs (MEVs) vitro followed by validation an experimental stroke model. Following pro-inflammatory activation, MEVs contain the protein TMEM119 alongside...
Abstract INTRODUCTION Accurate testing for Alzheimer's disease (AD) represents a crucial step therapeutic advancement. Currently, tests are expensive and require invasive sampling or radiation exposure. METHODS We developed nanoscale flow cytometry (nFC)‐based assay of extracellular vesicles (EVs) to screen biomarkers in plasma from mild cognitive impairment (MCI), AD, controls. RESULTS Circulating amyloid beta (Aβ), tau, phosphorylated tau (p‐tau)181, p‐tau231, p‐tau217, p‐tauS235,...
Clinical data suggest that Alzheimer disease (AD) and stroke together potentiate cognitive impairment. Inflammatory mechanisms are involved in AD pathology may be the mediator between toxicity.AD was modeled by cerebroventricular injections of beta-amyloid (Abeta[25-35]) subcortical lacunar infarcts striatal endothelin injections. were examined using immunohistochemical analysis. Memory motor tasks assessed Montoya staircase test.Abeta elicited increases pathological inflammatory correlates...
Secondary damage processes, such as inflammation and oxidative stress, can exacerbate an ischemic lesion spread to adjacent brain regions. Yet, few studies investigate how regions remote from the infarct could also suffer degeneration in aftermath of a stroke. To find out what extent far-remote are affected after stroke, we used bilateral endothelin-1-induced prefrontal rat model. Brain posterior cortical were analyzed for ongoing neurodegeneration using FluoroJadeB neuroinflammation Iba1...
The aging brain is often characterized by the presence of multiple comorbidities resulting in synergistic damaging effects as demonstrated through interaction Alzheimer's disease (AD) and stroke. Gangliosides, a family membrane lipids enriched central nervous system, may have mechanistic role mediating brain's response to injury their expression altered number states. Matrix-Assisted Laser Desorption Ionization (MALDI) Imaging Mass Spectrometry (IMS) was used study A-series ganglioside...
1,6-Diphenyl-1,3,5-hexatriene (DPH) is a commonly used fluorescence probe for studying cell membrane-lipids due to its affinity toward the acyl chains in phospholipid bilayers. In this work, we investigated use matrix-assisted laser desorption/ionization (MALDI) as new matrix mass spectrometry imaging (MSI) of mouse and rat brain tissue. DPH exhibits very minimal matrix-induced background signals analysis small molecules (below m/z 1000). negative ion mode, permits highly sensitive detection...