A5036114336- Cellular transport and secretion
- Retinal Development and Disorders
- Fungal and yeast genetics research
- Microtubule and mitosis dynamics
- Plant-Microbe Interactions and Immunity
- Distributed systems and fault tolerance
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Calcium signaling and nucleotide metabolism
- Software System Performance and Reliability
- Protein Kinase Regulation and GTPase Signaling
- Erythrocyte Function and Pathophysiology
- Parallel Computing and Optimization Techniques
- Signaling Pathways in Disease
- Nerve injury and regeneration
- Cloud Computing and Resource Management
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Toxin Mechanisms and Immunotoxins
- Plant Pathogens and Fungal Diseases
- Receptor Mechanisms and Signaling
- Mycotoxins in Agriculture and Food
- Endoplasmic Reticulum Stress and Disease
- Lipid Membrane Structure and Behavior
- Ubiquitin and proteasome pathways
University of Oklahoma Health Sciences Center
2015-2024
China University of Petroleum, Beijing
2024
Henan Provincial Center for Disease Control and Prevention
2021-2023
Zhengzhou University
2021-2023
First Affiliated Hospital of Zhengzhou University
2021-2023
Tsinghua University
2023
Industrial and Commercial Bank of China
2022
University of Chicago
2017-2021
National Clinical Research Center for Digestive Diseases
2021
Hebei University of Technology
2019
Adenosine diphosphate-ribosylation factor 6 (ARF6), ARF6 mutants, and ARF1 were transiently expressed in Chinese hamster ovary cells, the effects on receptor-mediated endocytosis assessed. Overexpressed localized to cell periphery led a redistribution of transferrin receptors surface decrease rate uptake transferrin. Similar results obtained when mutant defective guanosine triphosphate hydrolysis was expressed. Expression dominant negative mutant, ARF6(T27N), resulted an intracellular...
Abstract Photonic computing enables faster and more energy-efficient processing of vision data 1–5 . However, experimental superiority deployable systems remains a challenge because complicated optical nonlinearities, considerable power consumption analog-to-digital converters (ADCs) for downstream digital vulnerability to noises system errors 1,6–8 Here we propose an all-analog chip combining electronic light (ACCEL). It has systemic energy efficiency 74.8 peta-operations per second watt...
Overexpression of rab5 via a Sindbis virus vector resulted in 2-3-fold stimulation horseradish peroxidase uptake BHK-21 cells. Based on this functional assay activity, we conducted extensive mutational analysis the structure-function relationship rab5. A total 21 deletion and substitution mutations were created their effects activity examined. Deletion entire C-terminal tetrapeptide motif CCSN abolished activity. mutant with last three residues deleted, however, showed residual Truncation...
Mild cognitive impairment (MCI) is a prodromal stage of dementia. Understanding the mechanistic changes from healthy aging to MCI critical for comprehending disease progression and enabling preventative intervention.
Rac1 is a small GTPase involved in actin cytoskeleton organization and polarized cell growth many organisms. In this study, we investigate the biological function of MgRac1, homolog Magnaporthe grisea. The Mgrac1 deletion mutants are defective conidial production. Among few conidia generated, they malformed appressorial formation consequently lose pathogenicity. Genetic complementation with native MgRac1 fully recovers all these phenotypes. Consistently, expression dominant negative allele...
Nerve growth factor (NGF) induces neurite outgrowth and differentiation in a process that involves NGF binding to its receptor TrkA endocytosis of the NGF-TrkA complex into signaling endosomes. Here, we find biogenesis endosomes requires inactivation Rab5 block early endosome fusion. Expression dominant-negative mutants enhanced NGF-mediated outgrowth, whereas constitutively active mutant or Rabex-5 inhibited this process. Consistently, sustained activation on Furthermore, treatment rapidly...
Rab GTPases represent the largest subfamily of Ras-related small and regulate membrane trafficking. Vesicular transport is a general mechanism that governs intracellular trafficking along endocytic exocytic pathways in all eukaryotic cells. Fusarium graminearum filamentous fungus causes devastating economically important head blight wheat related species. The vesicular not well understood, little known about F. graminearum. In this study, we systematically characterized eleven FgRabs by live...
The retromer mediates protein trafficking through recycling cargo from endosomes to the trans-Golgi network in eukaryotes. However, role of such events during pathogen-host interaction remains unclear. Here, we report that cargo-recognition complex (MoVps35, MoVps26 and MoVps29) is essential for appressorium-mediated host penetration by Magnaporthe oryzae, causal pathogen blast disease rice. Loss function blocked glycogen distribution turnover lipid bodies, delayed nuclear degeneration...
Rabex-5 targets to early endosomes and functions as a guanine nucleotide exchange factor for Rab5. Membrane targeting is critical activate Rab5 on in the cell. Here, we report identification of Rab22 binding site direct recruitment activation Rab5, establishing Rab22-Rab5 signaling relay promote endosome fusion. guanosine 5'-O-(3-thio)triphosphate-loaded form, but not diphosphate-loaded binds endosomal domain (residues 81-230) pull-down assays. Rab22-containing endosomes, knockdown by short...
Concurrency bugs are hard to find, reproduce, and debug. They often escape rigorous in-house testing, but result in large-scale outages production. Existing concurrency-bug detection techniques unfortunately cannot be part of industry's integrated build test environment due some open challenges: how handle code developed by thousands engineering teams that uses a wide variety synchronization mechanisms, report little/no false positives, avoid excessive testing resource consumption.
Fusarium graminearum is a fungal pathogen that causes head blight (FHB) in wheat and barley. Autophagy highly conserved vacuolar degradation pathway essential for cellular homeostasis which Atg9 serves as multispanning membrane protein important generating membranes the formation of phagophore assembly site. However, mechanism autophagy or autophagosome phytopathogens awaits further clarifications. In this study, we identified characterized homolog (FgAtg9) F. by live cell imaging,...
Activation of Ras stimulates cell surface membrane ruffling and pinocytosis. Although seen as coupled events, our study demonstrates that pinocytosis are regulated by distinct signal transduction pathways. controls via the small GTPase Rac. In BHK-21 cells, expression constitutively active Rac1(G12V) mutant, a Sindbis virus vector, resulted in dramatic stimulation without affecting uptake horseradish peroxidase. Expression Ha-Ras(G12V), an activated stimulated both peroxidase uptake. The...
Eukaryotic lanthionine synthetase C-like protein 1 (LanCL1) is homologous to prokaryotic cyclases, yet its biochemical functions remain elusive. We report the crystal structures of human LanCL1, both free and complexed with glutathione, revealing glutathione binding a zinc ion at putative active site formed by conserved GxxG motifs. also demonstrate in vitro affinity analysis that LanCL1 binds specifically SH3 domain signaling protein, Eps8. Importantly, expression mutants defective Eps8...
After internalization, transmembrane receptors (TMRs) are typically recycled back to the cell surface or targeted for degradation. Efficient TMR trafficking is critical regulation of several processes, including signal transduction pathways, development, and disease. Here, we determined that angiogenic receptor neuropilin-1 (NRP-1) abrogated by liver kinase B1 (LKB1), a serine-threonine calcium calmodulin family. We found aberrant NRP-1 expression in tumor cells from patients with lung...
Endocytosis contributes to axon collapse mediated by a repulsive guidance cue in the developing brain.