A5032230423- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Cardiac Ischemia and Reperfusion
- Organ Transplantation Techniques and Outcomes
- Anesthesia and Neurotoxicity Research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neuroscience and Neuropharmacology Research
- Erythrocyte Function and Pathophysiology
- Wound Healing and Treatments
- Ion Channels and Receptors
- Nitric Oxide and Endothelin Effects
- Calcium signaling and nucleotide metabolism
- Blood properties and coagulation
- Reconstructive Surgery and Microvascular Techniques
- Clinical Nutrition and Gastroenterology
- Exercise and Physiological Responses
- Neurogenetic and Muscular Disorders Research
- Lymphatic Disorders and Treatments
- Heart Rate Variability and Autonomic Control
- Insect and Pesticide Research
- Nicotinic Acetylcholine Receptors Study
- Plant Stress Responses and Tolerance
- Laser Applications in Dentistry and Medicine
- Rheology and Fluid Dynamics Studies
- Muscle and Compartmental Disorders
University of Debrecen
2010-2024
Transient receptor potential cation channel subfamily M member 4 (TRPM4) is a Ca2+-activated nonselective that mediates membrane depolarization. Although, current with the hallmarks of TRPM4-mediated has been previously reported in pancreatic acinar cells (PACs), role TRPM4 regulation cell function not yet explored. In present study, we identify this and describe its context Ca2+ signaling PACs using pharmacological tools TRPM4-deficient mice. We found significant was sensitive to inhibitors...
BACKGROUND:Intestinal ischemia-reperfusion (I/R) is a potentially life-threatening situation and its pathomechanism not fully understood yet. OBJECTIVE:To investigate the early micro-rheological, microcirculatory morphological consequences of intestinal I/R in rat model. METHODS:CD rats we re anesthetized subjected to Control (n = 7) or groups. Left femoral artery cannulation median laparotomy were performed. In group superior mesenteric was clamped for 30 minutes. Blood samples taken before...
Dantrolene is a ryanodine receptor (RyR) inhibitor, which used to relax muscles in malignant hyperthermia syndrome. Although dantrolene binds the RyR protein, its mechanism of action unknown, mainly because controversial results showing that inhibited Ca<sup>2+</sup> release from intact fibers and sarcoplasmic reticulum (SR) vesicles, but failed inhibit single channel currents bilayers. Accordingly, it was concluded an important factor for dantrolene's lost during purification procedure RyR....
Micro-rheological relations of renal ischemia-reperfusion (I/R) have not been completely elucidated yet. Concerning anti-inflammatory agents, it is supposed that sigma-1 receptor agonist N,N-dimethyl-tryptamin (DMT) can be useful to reduce I/R injury.To investigate the micro-rheological and metabolic parameters, effects DMT in rats.In anesthetized rats from median laparotomy both kidneys were exposed. In Control group (n = 6) no other intervention happened. 10) right vessels ligated after 60...
Voltage‐clamp fluorometry (VCF) enables the study of voltage‐sensitive proteins through fluorescent labeling accompanied by ionic current measurements for voltage‐gated ion channels. The heterogeneity signal represents a significant challenge in VCF. VCF depends on where cysteine mutation is incorporated, making it difficult to compare data among different mutations and studies standardize their interpretation. We have recently shown that originates from quenching amino acids vicinity...
Remote ischemic preconditioning (RIPC) can be protective against the damage. However, there is no consensus on optimal amount of tissue, number and duration cycles, timing preconditioning. The hemorheological background process also unknown.To investigate effects remote organ micro-rheological parameters during liver ischemia-reperfusion in rats.In anesthetized rats 60-minute partial ischemia was induced with 120-minute reperfusion (Control, n = 7). In preconditioned groups a tourniquet...
Physiological muscle contraction requires an intact ligand gating mechanism of the ryanodine receptor 1 (RyR1), Ca2+-release channel sarcoplasmic reticulum. Some mutations impair and thus cause disease. The RyR1 mutation T4706M is linked to a myopathy characterized by weakness. Although, low expression protein can explain in part symptoms, little known about function channels with this mutation. In order learn whether alters manner that account for observed we examined isolated from mice...
Flap hypoperfusion or ischemia-reperfusion (I/R) may occur during preparation-transposition procedures and by postoperative thrombotic complications. Behind the microcirculatory disturbances micro-rheological alterations are also supposed.We aimed to investigate groin flap I/R with following-up parameters.Anesthetized rats were subjected Control groups. Groin flaps prepared bilaterally, pedicled on superficial epigastric vessels. In group re-sutured after one hour, while in microvascular...
Growing number of clinical and experimental data reflect to the gender differences hemorheological parameters. However, little is known about potential effect gonadectomy consequent changes in sex hormone concentration. Adult, same-aged male female rats were involved study. In control group no surgical intervention was performed. gonadectomized (GoE) groups bilateral orchidectomy or ovariectomy completed. Body weight measurement blood sampling carried out 1st, 2nd 3rd postoperative months....
Purpose: To compare early- and late-effect remote ischemic preconditioning (RIPC) by analysing the microcirculatory, hemodynamic histological changes in partial liver ischemia-reperfusion of rats. Methods: 60-minute ischemia followed 120-minute reperfusion was performed without (Control group, n=7) or with preconditioning. In RIPC groups a tourniquet applied around left thigh using 3 cycles 10-minute ischemia/10-minute reperfusion, one (RIPC-1, twenty-four hours (RIPC-24, before I/R....
Purpose Composite flaps used in reconstructive surgery may intra- and postoperatively suffer from hypoperfusion and/or ischemia-reperfusion influencing wound healing. We aimed to follow-up the effect of ischemia on adipocutaneous flaps' healing microcirculation. Methods In anesthetized rats groin were formed bilaterally. Control group repositioned sutured back. Ischemia-Reperfusion (I/R) before repositioning suturing flap pedicles clamped for 60 minutes. Laser Doppler (LD) fluxmetry...
In the steel-work rolled coils of low carbon steel plates must be unended before pickling on continuous mill. This was solved with butt welded joint by laser beam welding at ISD DUNAFERR Dunai Vasmő ZRt. The 8 kW light power, near TEM00 mode beam’s final part optical system is a divided parabolic mirror, which creates two focal spot in region. interest productivity rising, bands’ ends have to highest speed as far possible. seams tolerate repeated bends mill without cracking. optimum...
In this study we performed the comprehensive pharmacological analysis of two stereoisomers 4-chloro-meta-cresol (4CMC), a popular ryanodine receptor (RyR) agonist used in muscle research. Experiments investigating Ca2+-releasing action isomers demonstrated that most potent isomer was 4-chloro-orto-cresol (4COC) (EC50 = 55 ± 14 μM), although 3-chloro-para-cresol (3CPC) more effective, as it able to induce higher magnitude Ca2+ flux from isolated terminal cisterna vesicles. Nevertheless, 3CPC...
Very recently, the diamide insecticide chlorantraniliprole was shown to induce Ca 2+ -release from sarcoplasmic reticulum (SR) vesicles isolated mammalian skeletal muscle through activation of SR channel ryanodine receptor.As this result raises severe concerns about safety chemical, we aimed learn more its action.To end, single-channel analysis performed, which showed that induced high-activity bursts opening accounts for -releasing action described before.