A5056911997- Analytical Chemistry and Chromatography
- Tendon Structure and Treatment
- Bone and Dental Protein Studies
- Foot and Ankle Surgery
- Oral microbiology and periodontitis research
- Collagen: Extraction and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Proteins in Food Systems
- Protein purification and stability
- Pulmonary Hypertension Research and Treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nitric Oxide and Endothelin Effects
- Cell Adhesion Molecules Research
- Lower Extremity Biomechanics and Pathologies
- Advanced Glycation End Products research
- S100 Proteins and Annexins
- Meat and Animal Product Quality
- Data Management and Algorithms
- Periodontal Regeneration and Treatments
- Connective tissue disorders research
- Electrospun Nanofibers in Biomedical Applications
- Molecular Biology Techniques and Applications
Czech Academy of Sciences
2016-2025
Czech Academy of Sciences, Institute of Physiology
2016-2025
Centre of Civil Engineering (Czechia)
2009
Charles University
2001-2008
Cardiovascular Research Center
2006-2008
Institute of Clinical and Experimental Medicine
2003-2004
Heidelberg University
1996
Intrinsic nanocrystalline diamond (NCD) films have been proven to be promising substrates for the adhesion, growth and osteogenic differentiation of bone-derived cells. To understand role various degrees doping (semiconducting metallic-like), NCD were deposited on silicon by a microwave plasma-enhanced CVD process their boron was achieved adding trimethylboron CH4:H2 gas mixture, B∶C ratio 133, 1000 6700 ppm. The room temperature electrical resistivity decreased from >10 MΩ (undoped films)...
Proteomic analysis of the human body is a significant recent scientific endeavour. In this study, we investigated proteomic profile dentin using modern analytical and mass spectrometric techniques. Five healthy permanent molars from five adults were cut, pulverized, denaturated with guanidine buffer, demineralized EDTA buffer. The extracted proteins analysed by gel electrophoresis ( SDS ‐ PAGE two‐dimensional electrophoresis), digested trypsin, separated liquid chromatography/high‐resolution...
The protein composition of the insoluble avian eggshell matrix was studied. determination these proteins in water (EDTA-insoluble) carried out using enzymatic cleavage followed by a high-performance liquid chromatography–mass spectrometry method. influence various enzymes on splitting also distribution depends type layer (localization within eggshell): ovocalyxin-32 found mainly outer (the cuticle); ovocleidin-116 and 17 ovocalyxin-36 were throughout whole eggshell, whereas ovalbumin only...
Chronic sojourn in hypoxic environment results the structural remodeling of peripheral pulmonary arteries and hypertension. We hypothesize that pathogenesis changes vascular structure is related to increase radical production induced by lung tissue hypoxia. Hypoxia primes alveolar macrophages produce more hydrogen peroxide. Furthermore, increased release oxygen radicals other cells cannot be excluded. Several recent reports demonstrate oxidant damage lungs exposed chronic The nitric oxide...
Abstract A method for the determination and quantification of collagen types I–V in rat tissues has been developed. This is based on fragmentation by cyanogen bromide followed trypsin digestion. After that, HPLC‐MS/MS (HPLC coupled to an IT mass spectrometer) analyses resulting peptide mixtures (peptide maps) were performed. Specific peptides each type selected. According online databases, these are present human, bovine, collagens. As a result, this can be potentially applied other species'...
Endocardial fibroelastosis (EFE) is a diffuse thickening of the ventricular endocardium, causing myocardial dysfunction and presenting as unexplained heart failure in infants children. One postulated causes persistent increased wall tension ventricles.To examine whether reduced pressure chick model hypoplastic left syndrome (HLHS) induced by atrial ligation (LAL) at embryonic day (ED) 4 associated with EFE later stages, fibrosis was evaluated histology immunoconfocal microscopy mass...
The slowly metabolized proteins of the extracellular matrix, typically collagen and elastin, accumulate reactive metabolites through uncontrolled non-enzymatic reactions such as glycation or products arising from reaction unsaturated long chain fatty acid (possessing aldehydic groups). A typical example these changes is formation advanced end-products (AGEs), resulting carbohydrates with free amino group proteins. accumulation AGEs structural alterations cause altered tissue properties...
ABSTRACT Idiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to group fibro‐proliferative disorders but its origin remains unknown. Our study aimed achieve first complex proteomic comparison clubfoot contracted tissue foot (medial side; n = 16), with non‐contracted (lateral 13). We used label‐free mass spectrometry quantification immunohistochemistry. Seven proteins were observed be significantly upregulated in medial side (asporin,...
Abstract An ideal decellularized allogenic or xenogeneic cardiovascular graft should be capable of preventing thrombus formation after implantation. The antithrombogenicity the is ensured by a confluent endothelial cell layer formed on its surface. Later repopulation and remodeling scaffold patient’s cells result in living autologous tissue. In work presented here, porcine pericardium scaffolds were modified growing fibrin mesh surface inside scaffolds, attaching heparin human vascular...
Avian eggshell matrix proteins were studied by two analytical approaches. Peptide mapping was done trypsin and pepsin followed collagenase cleavage; analyses carried out capillary electrophoresis reversed-phase high-performance liquid chromatography (HPLC). Comparison of peptide maps obtained both methods revealed a complex mixture peptides in the insoluble layers eggshell; it concluded that there are at least three different protein/peptide avian (cuticle, palisade, mammillary layer)....
Proteomic analysis was performed in post-nuclear supernatant (PNS) and Percoll-purified membranes (PM) prepared from fore brain cortex of rats exposed to increasing doses morphine (10-50 mg/kg) for 10 days.In PNS, the up (↑)- or down (↓)-regulated proteins exhibiting largest morphine-induced change were selected, excised manually gel identified by MALDI-TOF MS/MS: 1-(gi|148747414, Guanine deaminase), ↑2.5×; 2-(gi|17105370, Vacuolar-type proton ATP subunit B, isoform), ↑2.6×; 3-(gi|1352384,...
Autologous and allogenic human pericardia used as biomaterials for cardiovascular surgery are traditionally crosslinked with glutaraldehyde. In this work, we have evaluated the resistivity to collagenase digestion cytotoxicity of pericardium various concentrations glutaraldehyde in comparison by genipin, nordihydroguaiaretic acid, tannic unmodified pericardium. Crosslinking retained wavy-like morphology native visualized second harmonic generation microscopy. The products were analyzed using...
Abstract Valvular heart disease leads to ventricular pressure and/or volume overload. Pressure overload fibrosis, which might regress with its resolution, but the limits and details of this reverse remodeling are not known. To gain more insight into extent nature cardiac fibrosis in valve disease, we analyzed needle biopsies taken from interventricular septum patients undergoing surgery for replacement focusing on expression distribution major extracellular matrix protein involved process....