A5089655445- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Synthesis and Characterization of Heterocyclic Compounds
- Diamond and Carbon-based Materials Research
- Phenothiazines and Benzothiazines Synthesis and Activities
- Body Contouring and Surgery
- 3D Printing in Biomedical Research
- Collagen: Extraction and Characterization
- Metal and Thin Film Mechanics
- Lymphatic System and Diseases
- Microfluidic and Bio-sensing Technologies
- biodegradable polymer synthesis and properties
- Orthopaedic implants and arthroplasty
- Periodontal Regeneration and Treatments
- Graphene and Nanomaterials Applications
- Wound Healing and Treatments
- Tribology and Wear Analysis
- Polymer Nanocomposites and Properties
- Congenital heart defects research
- Advanced Sensor and Energy Harvesting Materials
- Analytical Methods in Pharmaceuticals
- Spectroscopy Techniques in Biomedical and Chemical Research
- Silk-based biomaterials and applications
Czech Academy of Sciences
2016-2024
Czech Academy of Sciences, Institute of Physiology
2016-2024
Brno University of Technology
2022
Charles University
2018-2021
Galectin-3 (Gal-3) is a β-galactoside-binding protein that influences various cell functions, including adhesion. We focused on the role of Gal-3 as an extracellular ligand mediating cell-matrix used human adipose tissue-derived stem cells and umbilical vein endothelial are promising for vascular tissue engineering. found these naturally contained their surface inside cells. Moreover, they were able to associate with exogenous added culture medium. This association was reduced β-galactoside...
The opinion regarding the origin of adult stem cells that should be used for living bone construct generation is strongly divided in scientific community. In this study, potential chitosan/β-1,3-glucan/hydroxyapatite (chit/glu/HA) material as a scaffold regeneration applications was evaluated by behaviour comparison derived from both origins-adipose mesenchymal cell (ADSC) tissue and marrow (BMDSCs). case ADSC isolation, low high negative pressures were applied during liposuction procedure...
Engineering artificial skin constructs is an ongoing challenge. An ideal material for hosting cells still to be discovered. A promising candidate low-cost cellulose, which commonly fabricated in the form of a mesh and applied as wound dressing. Unfortunately, structure topography current cellulose meshes are not optimal cell growth. To enhance surface physicochemical properties commercially available mesh, we coated with wood-derived nanofibrils (CNFs). Three different types coatings...
Diamond-like carbon (DLC) layers are known for their high corrosion and wear resistance, low friction, biocompatibility. However, it is often necessary to dope DLC with additional chemical elements strengthen adhesion the substrate. Ti-DLC (doped 0.4, 2.1, 3.7, 6.6, 12.8 at.% of Ti) were prepared by dual pulsed laser deposition, pure DLC, glass, polystyrene (PS) used as controls. In vitro cell–material interactions investigated an emphasis on cell adhesion, proliferation, osteogenic...
Adipose tissue-derived stromal cells (ADSCs) have great potential for cell-based therapies, including tissue engineering. However, various factors can influence the characteristics of isolated ADSCs. We studied harvesting site, i.e., inner thigh (n = 3), outer (. revealed higher initial cell yields from region than abdomen region. Negative pressure did not region, whereas were under high negative low pressure. In subsequent passage, in general, no significant relationship was identified...
Abstract An ideal decellularized allogenic or xenogeneic cardiovascular graft should be capable of preventing thrombus formation after implantation. The antithrombogenicity the is ensured by a confluent endothelial cell layer formed on its surface. Later repopulation and remodeling scaffold patient’s cells result in living autologous tissue. In work presented here, porcine pericardium scaffolds were modified growing fibrin mesh surface inside scaffolds, attaching heparin human vascular...
Ti-doped hydrogen free diamond-like carbon (DLC) layers of dopation up to ~25 at.% were prepared by dual beam pulsed laser deposition (PLD) using two excimer lasers. The arrangement allows continuous fine tuning dopant concentration on a large scale and flexibility. Si(1 0 0) Ti6Al4V substrates at room temperature. surface morphology, mechanical properties, bonds, composition, morphology human osteoblast-like Saos-2 cells, their metabolic activity production osteocalcin, marker osteogenic...
Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed tissue engineering. We have developed a new simple method heterologous expression purification VEGF-A165 FGF-2 in yeast system Pichia pastoris. The biological activity factors was assessed cultures human porcine adipose tissue-derived stem cells (ADSCs) umbilical vein (HUVECs). When added into culture medium, stimulated proliferation only...
Herein, a renewed prominence towards the synthesis of poly(alkylene citrate) (PAC) biomaterials and their detailed chemical, structural mechanical characterization has been reported.
The aim of our research was to study the behaviour adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely pristine PLLA, plasma-treated PLLA grafted with polyethylene glycol (PEG), dextran (Dex), tissue culture polystyrene (PS) control. On these materials, ADSCs were biochemically differentiated towards VSMCs by a medium supplemented TGFβ1, BMP4 ascorbic acid (i.e. differentiation medium). cultured in...
Diamond-like carbon (DLC) layers are known for their high corrosion and wear resistance, low friction, biocompatibility. However, it is often necessary to dope DLC with additional chemical elements strengthen adhesion the substrate. Ti-DLC (doped 0.4, 2.1, 3.7, 6.6, 12.8 at.% of Ti) were prepared by dual pulsed laser deposition, pure DLC, glass, polystyrene (PS) used as controls. In vitro cell-material interactions investigated an emphasis on cell adhesion, proliferation, osteogenic...
Beta-titanium alloys are promising materials for bone implants due to their advantageous mechanical properties. For enhancing the interaction of cells with this perspective material, we developed a ferroelectric barium titanate (BaTiO3) coating on Ti39Nb alloy by hydrothermal synthesis. This was analyzed scanning electron and Raman microscopy, X-ray diffraction, piezoresponse force photoelectron spectroscopy, nanoindentation, roughness measurement. Leaching experiments in saline solution...
One of the major goals vascular tissue engineering is to develop much-needed materials that are suitable for use in small-diameter grafts. Poly(1,8-octamethylene citrate) can be considered manufacturing small blood vessel substitutes, as recent studies have demonstrated this material cytocompatible with adipose tissue-derived stem cells (ASCs) and favors their adhesion viability. The work presented here focused on modifying polymer glutathione (GSH) order provide it antioxidant properties,...
Hydrogels, and not only natural polysaccharide hydrogels, are substances capable of absorbing large amounts water physiological fluids. In this study, we set out to optimize the process for preparing polyvinyl alcohol (PVA) hydrogels. Subsequently, doped PVA foils with cellulose powder, poly(ethylene glycol) (PEG) or gold nanoparticles in PEG colloid solutions (Au). The were then modified a plasma discharge improve their biocompatibility. properties studied by various analytical methods. use...
Scaffolds made of degradable polymers, such as collagen, polyesters or polysaccharides, are promising matrices for fabrication bioartificial vascular grafts patches. In this study, collagen isolated from porcine skin was processed into a gel, reinforced with particles and incorporated adipose tissue-derived stem cells (ASCs). The cell-material constructs were then incubated in DMEM medium 2% FS (DMEM_part), added polyvinylalcohol nanofibers (PVA_part sample), ASCs differentiation towards...