A5057389424- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Sports injuries and prevention
- Mesenchymal stem cell research
- RNA and protein synthesis mechanisms
- Cardiovascular Effects of Exercise
- RNA Research and Splicing
- Cardiac Valve Diseases and Treatments
- Connective tissue disorders research
- Periodontal Regeneration and Treatments
- Graphene and Nanomaterials Applications
- Biopolymer Synthesis and Applications
- Cardiac Structural Anomalies and Repair
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Lower Extremity Biomechanics and Pathologies
- Orthopaedic implants and arthroplasty
- Bacteriophages and microbial interactions
- Cardiac and Coronary Surgery Techniques
- Cardiovascular and exercise physiology
- Bone fractures and treatments
- Knee injuries and reconstruction techniques
- Cardiac Fibrosis and Remodeling
- Osteoarthritis Treatment and Mechanisms
University of Wisconsin–Madison
2016-2025
Pain and Rehabilitation Medicine
2022-2024
Mayo Clinic
2018-2023
Mayo Clinic in Arizona
2018-2019
Fixed pericardial tissue is commonly used for commercially available xenograft valve implants, and has proven durability, but lacks the capability to remodel grow. Decellularized porcine promise outperform fixed remodel, decellularization process been shown damage collagen structure reduce mechanical integrity of tissue. Therefore, a comparison uniaxial tensile properties was performed on decellularized, decellularized-sterilized, fixed, native versus leaflet cusps. The results...
Abstract The reliance of current COVID‐19 mRNA lipid nanoparticles on cold storage increases the cost and reduces access to vaccines. As therapeutic expands other clinical opportunities, better methods stabilize medicines during shipping, storage, delivery are needed. This work reviews advances in design with a focus codon optimization, chemical modifications, RNA structures. Additionally, technologies promoting nanoparticle stabilization including ionizable lipids, excipients,...
Abstract Development of strategies for non‐viral gene delivery targeted to bone can be used pathologies. Applications include rheumatologic disease, metastatic disease the bone, pathologies with deficits in hematopoiesis, osteomyelitis, and regenerative medicine. However, specific tissues like is challenging. Here mRNA LNPs (lipid nanoparticles) decorated a biomimetic hydroxyapatite‐binding peptide (HABP‐LNP) are described, which show improved binding minerals compared sham conjugated...
Spinal cord injury often results in devastating consequences for those afflicted, with very few therapeutic options. A central element of spinal injuries is astrogliosis, which forms a glial scar that inhibits neuronal regeneration post-injury. Chondroitinase ABC (ChABC) an enzyme capable degrading chondroitin sulfate proteoglycan (CSPG), the predominant extracellular matrix component scar. However, poor protein stability remains challenge its use. Messenger RNA (mRNA) delivery emerging gene...
Fractures continue to be a global economic burden as there are currently no osteoanabolic drugs approved accelerate fracture healing. In this study, we aimed develop an therapy which activates the Wnt/β-catenin pathway, molecular driver of endochondral ossification. We hypothesize that using mRNA-based therapeutic encoding β-catenin could promote cartilage bone transformation formation by activating canonical Wnt signaling pathway in chondrocytes. To optimize delivery platform built on...
Recruitment of endothelial cells to cardiovascular device surfaces could solve issues thrombosis, neointimal hyperplasia, and restenosis. Since current targeting strategies are often nonspecific, new technologies allow for site-specific cell localization capture
Commercially available heart valves have many limitations, such as a lack of remodeling, risk calcification, and thromboembolic problems. Many state-of-the-art tissue-engineered (TEHV) rely on recellularization to allow remodeling transition mechanical behavior native tissues. Current in vitro testing is insufficient characterizing soon-to-be living valve due this change response; thus, it imperative understand the performance an situ valve. However, complex vivo environment, difficult...
Periostin, originally named osteoblast-specific factor 2 (OSF-2) has been identified primarily in collagen rich, biomechanically active tissues where its role implicated mechanisms to maintain the extracellular matrix (ECM), including fibrillogenesis and crosslinking. It is well documented that periostin plays a wound healing scar formation after injury, part, by promoting cell proliferation, myofibroblast differentiation, and/or fibrillogenesis. Given significance of other forming models,...
PurposeThe purpose of our study was to identify key molecular components within the femoroacetabular impingement (FAI) hip and compare findings between males females across varying age groups.MethodsAll patients undergoing arthroscopy for syndrome (FAIS) without dysplasia were included. During arthroscopy, loose articular cartilage, excess synovium, damaged labral tissue, minimal adipose tissue debrided only as needed visualization repair purposes collected. Tissue processed used qPCR. Genes...
<h3>Background</h3> Heterologous vaccine strategies, priming with one type of and boosting another, have shown improvements in generating immune responses clinical preclinical studies for infectious diseases cancer treatment comparison to homologous immunization strategies. Here, we sought determine whether prime-boost strategies mRNA encapsulated lipid nanoparticles (LNP) a DNA (pDNA) vaccine, each encoding ovalbumin (OVA) as an antigen, could affect the expansion function CD8 T cells....
Patients who are 18-36 months post-anterior cruciate ligament reconstruction (ACLR) have greater joint power asymmetries during a countermovement jump (CMJ), suggestive of residual neuromuscular deficits. Characterizing in CMJ kinetics the first year post-ACLR may provide insight into return to sport decision making. PURPOSE: To identify between-limb differences among athletes 4-12 and determine if these affected by time post-surgery. METHODS: Sixteen collegiate within one performed while...
Background: The COVID-19 pandemic and related lockdowns in 2020 negatively impacted high school students, with increased depression anxiety especially athletes who participated team sports. For adolescents disabilities, physical activity provides mental health benefits; however, the impact of adaptive sports cancellations have not been thoroughly investigated. Hypothesis: Adaptive lower levels during a period restrictions will worse scores depression, anxiety, quality life. Methods:...