A5063335422- Bone fractures and treatments
- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Mesenchymal stem cell research
- Connective tissue disorders research
- Periodontal Regeneration and Treatments
- Orthopaedic implants and arthroplasty
- Bone Tissue Engineering Materials
- Body Composition Measurement Techniques
- Bone Metabolism and Diseases
- Extracellular vesicles in disease
- Electrical and Bioimpedance Tomography
- Hip disorders and treatments
- Hip and Femur Fractures
- Cancer-related gene regulation
- Telomeres, Telomerase, and Senescence
- Tendon Structure and Treatment
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Nuclear Structure and Function
- Orthopedic Infections and Treatments
- Total Knee Arthroplasty Outcomes
- Bone health and osteoporosis research
- Salivary Gland Disorders and Functions
- Bone health and treatments
University of California, San Francisco
2016-2025
Steadman Philippon Research Institute
2020-2024
Colorado State University
2022-2024
Steadman Clinic
2020-2024
Color (United States)
2024
University of California, Berkeley
2013-2023
Orthopaedic Trauma Association
2016-2023
Center for Neuroscience and Regenerative Medicine
2023
San Francisco General Hospital
2013-2022
AO Foundation
2022
Fractures heal predominantly through the process of endochondral ossification. The classic model ossification holds that chondrocytes mature to hypertrophy, undergo apoptosis and new bone forms by invading osteoprogenitors. However, recent data demonstrate transdifferentiate osteoblasts in growth plate during regeneration, yet mechanism(s) regulating this remain unknown. Here, we show a spatially-dependent phenotypic overlap between hypertrophic at chondro-osseous border fracture callus,...
Biological activity can be added to synthetic scaffolds by incorporating functional peptide sequences that provide enzyme-mediated degradation sites, facilitate cellular adhesion or stimulate signaling pathways.Poly(ethylene glycol) diacrylate is a popular base for tissue engineering because it creates hydrophilic environment chemically manipulated add this biological functionality.Furthermore, the acrylate groups allow encapsulation of cells using photopolymerization under physiological...
ABSTRACT Although bone has great capacity for repair, there are a number of clinical situations (fracture non-unions, spinal fusions, revision arthroplasty, segmental defects) in which auto- or allografts attempt to augment regeneration by promoting osteogenesis. Critical failures associated with current grafting therapies include osteonecrosis and limited integration between graft host tissue. We speculated that the underlying problem techniques is they promote through direct Here we...
Cartilage tissue engineering aims to replace damaged or diseased with a functional regenerate that restores joint function. Scaffolds are used deliver cells and facilitate development, but they can also interfere the structural assembly of cartilage matrix. Biodegradable scaffolds have been proposed as means improve matrix deposition biomechanical properties neocartilage. The challenge is designing appropriate degradation rates, ideally such scaffold proportional deposition. In this study,...
Aging leads to several geriatric conditions including osteoporosis (OP) and associated frailty syndrome. Treatments for these are limited none target fundamental drivers of pathology, thus identifying strategies delay progressive loss tissue homeostasis functional reserve will significantly improve quality life in elderly individuals. A property aging is the accumulation senescent cells. Senescence a cell state defined by proliferative capacity, resistance apoptosis, release proinflammatory...
Significance Our results show that one single systemic administration of the β-adrenoreceptor blocker propranolol prior to femur osteotomy in psychosocially stressed mice prevents stress-induced bone marrow mobilization neutrophils and their immigration into early fracture hematoma, as well decrease bending stiffness bony bridging callus, which indicated a compromised healing mice. These findings offer possibilities for clinical translation patients suffering from posttraumatic stress...
Abstract Chronic conditions associated with aging have proven difficult to prevent or treat. Senescence is a cell fate defined by loss of proliferative capacity and the development pro‐inflammatory senescence‐associated secretory phenotype comprised cytokines/chemokines, proteases, other factors that promotes age‐related diseases. Specifically, an increase in senescent peripheral blood mononuclear cells (PBMCs), including T cells, like frailty, rheumatoid arthritis, bone loss. However, it...
Tissue engineering utilizes scaffolds containing chondrogenic cells to promote cartilage development at a clinically relevant scale, yet there remains limited understanding of the optimal conditions for inducing differentiation and matrix production. We investigated how cell density temporal exposure factors impacted chondrogenesis human mesenchymal stem (hMSCs) encapsulated in poly(ethylene glycol) diacrylate hydrogels. found maximal proteoglycan collagen production constructs seeded...
A type X collagen fragment derived from endochondral ossification and detected in blood is a marker for growth velocity.
There are currently no standardized methods for assessing fracture healing, with physicians relying on X-rays which only useful at later stages of repair. Using in vivo mouse models, we present the first evidence that microscale instrumented implants provide a route post-operative monitoring, utilizing electrical impedance spectroscopy (EIS) to track healing tissue high sensitivity. In this study, fixed long bone fractures external fixators and plates. EIS measurements taken across two...
Introduction Clinical and preclinical data suggest accelerated bone fracture healing in subjects with an additional traumatic brain injury (TBI). Mechanistically, altered metabolism neuro-endocrine regulations have been shown to influence formation after combined TBI, thereby increasing the content callus. However, early inflammatory response towards TBI has not investigated detail so far. This is of great importance, since phase known be essential for initiation downstream regenerative...
Abstract There are no therapies for reversing chronic organ degeneration. Non-healing degenerative wounds thought to be irreparable, in part, by the inability of tissue respond reparative stimuli. As such, treatments typically aimed at slowing degeneration or replacing cells through transplantation. Building on our previous studies showing acutely injured salivary glands, and specifically secretory acini, can regenerated, we reveal that non-healing, degenerating murine glands remain...
Bone marrow–derived mesenchymal stem cells (BM‐MSCs) are well established for their osteogenic potential but prone to senescence with aging or in vitro expansion. Drug treatments that reduce cellular may enhance the regenerative capacity of BM‐MSCs. This study investigates effects losartan and fisetin, both separately combination, on osteogenesis. Human BM‐MSCs were exposed low high concentrations each drug 24 h. Our findings showed high‐dose exhibited cytotoxicity, focusing subsequent...
Abstract Traumatic injuries are associated with significant acute pain and subsequent high risk of the development chronic pain. However, addressing after skeletal trauma presents a complex challenge to achieve effective relief that minimizes addiction does not interfere functional recovery. The Orthopaedic Trauma Association's 2023 Basic Science Focus Forum aimed bridge gap between basic science clinical outcomes an educational symposium on management designed foster collaboration provide...
Background: Meniscal vascular supply is an important determinant of its healing potential. It has been reported that only the peripheral 30% meniscus vascularized in cadavers aged 53 to 94 years; however, vascularity young patients, whom meniscal repair more often performed, unknown. Purpose: The primary objective was analyze and measure microvascular anatomy adult cadaveric specimens <35 years old. secondary assess angiogenic potential by quantifying regional gene expression a allograft...