A5026962260- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Diet and metabolism studies
- Multiple Myeloma Research and Treatments
- 3D Printing in Biomedical Research
- Facial Nerve Paralysis Treatment and Research
- Neurological disorders and treatments
- Cardiovascular Effects of Exercise
- Birth, Development, and Health
- Neuroscience and Neuropharmacology Research
- Pancreatic function and diabetes
- Epilepsy research and treatment
- Cardiovascular Function and Risk Factors
- MicroRNA in disease regulation
- Chemokine receptors and signaling
- Radiopharmaceutical Chemistry and Applications
- COVID-19 Clinical Research Studies
- Brain Metastases and Treatment
- Bone Metabolism and Diseases
- Glioma Diagnosis and Treatment
- Dental Implant Techniques and Outcomes
- Parkinson's Disease Mechanisms and Treatments
- Adipose Tissue and Metabolism
University of Trieste
2020-2023
University of Udine
2007-2023
University Hospital Galway
2022-2023
Ollscoil na Gaillimhe – University of Galway
2016-2022
Azienda Sanitaria Universitaria Integrata di Trieste
2020-2021
San Francisco General Hospital
2013-2017
University of California, San Francisco
2013-2017
University of Chieti-Pescara
2005
Istituto di Farmacologia Traslazionale
1996
Università Cattolica del Sacro Cuore
1987
Fractures heal predominantly through the process of endochondral ossification. The classic model ossification holds that chondrocytes mature to hypertrophy, undergo apoptosis and new bone forms by invading osteoprogenitors. However, recent data demonstrate transdifferentiate osteoblasts in growth plate during regeneration, yet mechanism(s) regulating this remain unknown. Here, we show a spatially-dependent phenotypic overlap between hypertrophic at chondro-osseous border fracture callus,...
ABSTRACT Although bone has great capacity for repair, there are a number of clinical situations (fracture non-unions, spinal fusions, revision arthroplasty, segmental defects) in which auto- or allografts attempt to augment regeneration by promoting osteogenesis. Critical failures associated with current grafting therapies include osteonecrosis and limited integration between graft host tissue. We speculated that the underlying problem techniques is they promote through direct Here we...
Although the role played by core transcription factor network, which includes c-Myc, Klf4, Nanog, and Oct4, in maintenance of embryonic stem cell (ES) pluripotency reprogramming adult cells is well established, its persistence function are still debated. To verify clarify these molecules function, we investigated expression pattern markers undifferentiated fully differentiated dental pulp (DPSC). A particular attention was devoted to intracellular localization stemness-associated isoform...
Adult stem cells have been proposed as an alternative to embryonic study multilineage differentiation in vitro and use therapy. Current culture media for isolation expansion of adult require the large amounts animal sera, but animal-derived reagents give rise some questions due real possibility infections severe immune reactions. For these reasons a clinical grade substitute sera is needed. We tested isolation, proliferation, morphology, stemness related marker expression, osteoblastic...
Periodontitis is a chronic infectious disease, characterized by the progressive loss of teeth's supporting tissues, affecting almost 40% entire adult population. An imbalance between localized gram-negative infection and an exaggerated host inflammatory response plays pivotal role in determining gingival tissue damage. Recent evidence suggests that effect periodontitis might not be limited just to oral cavity but it have systemic consequences. Indeed has also been associated with moderate...
After in vivo transplantation, mesenchymal stem cells (MSC) face an ischemic microenvironment, characterized by nutrient deprivation and reduced oxygen tension, which reduces their viability thus therapeutic potential. Therefore, MSC response to models of vitro ischemia is relevance for improving survival efficacy. The aim this study was understand the survival/adaptive mechanism that use respond extreme culture conditions. Specifically, effect a long-term starvation on human bone marrow...
Embryonic stem cell self-renewal properties are attributed to critical amounts of OCT4A, but little is known about its post-translational regulation. Sequence analysis revealed that OCT4A contains five putative ERK1/2 phosphorylation sites. Consistent with the hypothesis a substrate, we demonstrate interacts by using both in vitro GST pulldown and vivo co-immunoprecipitation assays. MS identified at Ser-111. To investigate possibility activation can enhance degradation, analyzed endogenous...
Abstract The rising incidence of non-ST-segment elevation myocardial infarction (NSTEMI) and associated long-term high mortality constitutes an urgent clinical issue. Unfortunately, the study possible interventions to treat this pathology lacks a reproducible pre-clinical model. Indeed, currently adopted small large animal models MI mimic only full-thickness, ST-segment-elevation (STEMI) infarcts, hence cater for investigation into therapeutics directed at subset MI. Thus, we develop ovine...
Multiple myeloma (MM) is a malignancy of terminally-differentiated plasma cells that develops mainly inside the bone marrow (BM) microenvironment. It well known autocrine and paracrine signals are responsible for progression this disease but precise mechanism contributions from single cell remain largely unknown. Mesenchymal stem (MSC) an important cellular component BM: they support MM growth by increasing its survival chemo-resistance, little about signaling pathways. Three-dimensional...
The ability of the zebrafish heart to regenerate following injury makes it a valuable model deduce why this capability in mammals is limited early neonatal stages. Although metabolic reprogramming and glycosylation remodeling have emerged as key aspects many biological processes, how they may trigger cardiac regenerative response still crucial question. Here, by using an up-to-date panel transcriptomic, proteomic glycomic approaches, we identify switch from mitochondrial oxidative...
Grafts of tissue-engineered bone represent a promising alternative in the treatment large and small defects. Current approaches are often badly tolerated by patients because invasiveness, ethical problems, culture, possibility infection. Autologous grafts have been indicated as solution to such problems. Because tissue availability, many proposed use cultured cells derived from marrow expanded culture induced differentiate tissue. Data reported literature show that it is possible produce...
Survivors after a myocardial infarction (MI), especially those with diabetes mellitus (DM), remain at high risk of further events. Identifying and treating factors that may influence survival open new therapeutic strategies. We assessed the impact on prognosis DM hypovitaminosis D (hypovitD), alone or combined. In this prospective, observational study, 1081 patients were enrolled surviving an MI divided into four groups according to their diabetic VitD status. The primary end-point was...
Abstract: l ‐Glutamate, NMDA, dl ‐α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole propionate (AMPA), and kainate (KA) increased the release of somatostatin‐like immunoreactivity (SRIF‐LI) from primary cultures rat hippocampal neurons. In Mg 2+ ‐containing medium, maximal effects (reached at ∼100 µ M ) amounted to 737% (KA), 722% (glutamate), 488% (NMDA), 374% (AMPA); apparent affinities were 22 39 41 70 (NMDA). The metabotropic receptor agonist trans ‐1‐aminocyclopentane‐1,3‐dicarboxylate did not...
Mesenchymal stem/stromal cells (MSC) have been employed successfully in immunotherapy and regenerative medicine, but their therapeutic potential is reduced considerably by the ischemic environment that exists after transplantation. The assumption preconditioning MSC to promote quiescence may result increased survival upon transplantation gaining popularity.The purpose of this work was evaluate anti-inflammatory effects human bone marrow (hBM-MSC) extracellular vesicles (EVs) grown isolated a...