A5061205863- Reproductive Biology and Fertility
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Animal Genetics and Reproduction
- Cardiovascular Effects of Exercise
- Pluripotent Stem Cells Research
- Genetics, Aging, and Longevity in Model Organisms
- Neuroendocrine regulation and behavior
- Reproductive Physiology in Livestock
- TGF-β signaling in diseases
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Stress Responses and Cortisol
- Developmental Biology and Gene Regulation
- Sperm and Testicular Function
- Birth, Development, and Health
- Mitochondrial Function and Pathology
- Wnt/β-catenin signaling in development and cancer
- Estrogen and related hormone effects
- Genetics and Physical Performance
- Receptor Mechanisms and Signaling
- Telomeres, Telomerase, and Senescence
- Cardiac tumors and thrombi
- Biomedical Ethics and Regulation
- Evolution and Paleontology Studies
Ollscoil na Gaillimhe – University of Galway
2005-2022
Therapeutics Clinical Research
2012
National University of Ireland
2007-2009
Teagasc - The Irish Agriculture and Food Development Authority
1994-1999
Food Safety Authority of Ireland
1995-1997
Proteus (United Kingdom)
1995
Novartis (Switzerland)
1995
The protein content of cattle oocytes and preimplantation embryos produced in vivo, from the two-cell to elongated blastocyst at day 16, was determined.From oocyte expanded stage (day 8), determination carried out on zona pellucida-enclosed embryos.Protein measured by Pierce Micro BCA assay.The mean 0.126 \g=m\g, with no significant increase (0.132 \g=m\g).Protein higher morula (0.183 \g=m\g; P < 0.05) a further (0.367 \ g=m\ g; 0.05).There 160-fold hatched 13 stage.Spherical, ovoid...
The insulin-like growth factor (IGF) family is essential for normal embryonic and development it highly conserved through vertebrate evolution. However, the roles that individual members of IGF play in have not been fully elucidated. This study focuses on role IGF-2 zebrafish development. Two igf-2 genes, igf-2a igf-2b, are present genome. Antisense morpholinos were designed to knock down both genes. neural cardiovascular defects morphant embryos then examined further using wholemount situ...
The ability of the zebrafish heart to regenerate following injury makes it a valuable model deduce why this capability in mammals is limited early neonatal stages. Although metabolic reprogramming and glycosylation remodeling have emerged as key aspects many biological processes, how they may trigger cardiac regenerative response still crucial question. Here, by using an up-to-date panel transcriptomic, proteomic glycomic approaches, we identify switch from mitochondrial oxidative...
Preimplantation development depends on multiple interactions between mother and embryo. The Epidermal Growth Factor Receptor (EGF-R) its ligands are potential components of the embryo-maternal cross-talk. Employing RT-PCR, in situ hybridization, immunohistochemistry, we investigated mRNA protein level expression EGF-R, (EGF), Transforming alpha (TGF-α), Heparin-binding EGF-like (HB-EGF) spherical elongating bovine blastocysts day 13 16 gestation, endometrium at gestation. EGF-R were detected...
The desmosomal armadillo protein plakophilin 2 is the only expressed in heart, and mutations human gene result arrhythmogenic right ventricular cardiomyopathy.To investigate loss of function, we knocked down by morpholino microinjection zebrafish.This resulted decreased heart rate, cardiac oedema, blood pooling, a failure to pattern correctly twisted tail.Co-injection mRNA rescued morphant phenotype, indicating specificity knockdown.Desmosome numbers were hearts plaque midline structures...
Acute intravenous administration of either clonidine (Clon) (50 μg kg −1 ) or desipramine (DMI) (5 mg elicited a pulse growth hormone (GH) and corticosterone secretion in conscious, unrestrained rats. The responses to DMI were similar those with Clon, except that the GH following was delayed not dose‐dependent. response inhibited by prior idazoxan (1 yohimbine (0.5 ), but atropine (10 sulpiride prazosin ). altered atropine, prazosin, augmented influenced sulpiride, given alone. Idazoxan...
The effects of immunizing cattle against either two peptides from the amino terminal peptide (αN) α43-subunit bovine inhibin on ovulation rate, gonadotrophin concentration and fertility were investigated. Two sequences αN-subunit (P1N, bIα-(8-20) P2N, bIα-(153-167)) synthesized conjugated to human serum albumin (HSA). Hereford-cross heifers (n = 5 per group) given an initial injection 3 mg one conjugates, followed by three booster injections (1.5 mg) at intervals 11 weeks. Control 5)...
The effects of direct and indirect activation adenylyl cyclase on the production intracellular extracellular cAMP cGMP by 13- to 16-day-old cattle embryos were determined. Embryos incubated for 2 h in a Krebs Ringer bicarbonate medium containing phosphodiesterase inhibitor isobutyl-methylxanthine, which stimulating agents forskolin (100 μmol l−1), cholera toxin (2 μg ml−1), or both added. Total (intraand extracellular) basal concentrations ranged from 6.65 ± 0.895 3.4 0.708 fmol μg−1 protein...
1. Rats were administered either desipramine (DMI) or sertraline daily at doses 7.5 mg kg-1 10 kg-1, i.p., respectively and the effects on functional state of hypothalamic neuroendocrine control mechanisms assessed by measurements plasma hormones following acute drug challenge. The treatment gross behaviour brain adrenoceptor density also determined. 2. Both DMI caused significant reduction in activity measured as ambulation rearing 14 days treatment. 3. All animals chronically cannulated...
Plakoglobin (gamma-catenin) and beta-catenin are major components of the adherens junctions can be localized to nucleus by activation Wnt signalling pathway. In addition, plakoglobin is also found in desmosomes, a vertebrate-specific cell-cell adhesion structure. expression localization were examined at protein level during zebrafish embryonic development Western blotting confocal microscopy. was expressed throughout embryo level. revealed that content increased between 12- 24-h...
Abstract Histopathology can reveal toxicant‐induced changes in the structure of a tissue or organ. A prerequisite for histopathological studies is sound knowledge morphology anatomical normal healthy state. Zebrafish larvae provide tool focused on hepatotoxicity at early stages development; therefore, fine organ should be well characterised. To date, liver 72 and 120 hr post‐fertilisation (hpf) has not been reported detail this study aimed to fill scientific gap. stereological approach...