A5034855480- Pregnancy-related medical research
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- Reproductive Biology and Fertility
- Cancer, Lipids, and Metabolism
- Glioma Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Thyroid Cancer Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Telomeres, Telomerase, and Senescence
- Injury Epidemiology and Prevention
- Estrogen and related hormone effects
- Genomics and Chromatin Dynamics
- Reproductive Physiology in Livestock
- HER2/EGFR in Cancer Research
- Effects and risks of endocrine disrupting chemicals
- Sperm and Testicular Function
- Testicular diseases and treatments
- Occupational Health and Performance
- Endoplasmic Reticulum Stress and Disease
- PI3K/AKT/mTOR signaling in cancer
- Toxic Organic Pollutants Impact
CancerCare Manitoba
2017-2025
University of Manitoba
2016-2025
Research Institute in Oncology and Hematology
2017-2025
Children's Hospital Research Institute of Manitoba
2009-2025
University of Winnipeg
2018-2021
University of Calgary
2016
Martin Luther University Halle-Wittenberg
1998-2012
In-Q-Tel
2009
University of Southern California
2009
Stem Cell Network
2009
Abstract Transforming growth factor- β 1 (TGF- ) is an important regulator of fibrogenesis in heart disease. In many other cellular systems, TGF- may also induce autophagy, but a link between its fibrogenic and autophagic effects unknown. Thus we tested whether or not -induced autophagy has regulatory function on fibrosis human atrial myofibroblasts (hATMyofbs). Primary hATMyofbs were treated with to assess for responses. Using immunoblotting, immunofluorescence transmission electron...
Lipoteichoic acids (LTAs) isolated from bacterial species, including Staphylococcus aureus, Streptococcus pyogenes A, Enterococcus faecalis, pneumoniae, and Listeria monocytogenes, were tested for their ability to stimulate the production of interleukin-1 beta (IL-1 beta), IL-6, tumor necrosis factor alpha in cultured human monocytes. LTAs S. aureus pneumoniae failed induce monokine when applied concentration range 0.05 5.0 micrograms/ml. However, several enterococcal species (0.5 5...
Abstract Brevinin‐2R is a novel non‐hemolytic defensin that was isolated from the skin of frog Rana ridibunda . It exhibits preferential cytotoxicity towards malignant cells, including Jurkat (T‐cell leukemia), BJAB (B‐cell lymphoma), HT29/219, SW742 (colon carcinomas), L929 (fibrosarcoma), MCF‐7 (breast adenocarcinoma), A549 (lung carcinoma), as compared to primary cells peripheral blood mononuclear (PBMC), T and human lung fibroblasts. overexpressing Bcl2, over‐expressing dominant‐negative...
Abstract The mevalonate (MEV) cascade is responsible for cholesterol biosynthesis and the formation of intermediate metabolites geranylgeranylpyrophosphate (GGPP) farnesylpyrophosphate (FPP) used in prenylation proteins. Here we show that MEV inhibitor simvastatin induced significant cell death a wide range human tumor lines, including glioblastoma, astrocytoma, neuroblastoma, lung adenocarcinoma, breast cancer. Simvastatin apoptotic via intrinsic pathway. In all cancer types tested,...
3-hydroxy-3-methyl-glutaryl-CoA reductase inhibitors (statins) are cholesterol-lowering drugs that exert other cellular effects and underlie their beneficial health effects, including those associated with myocardial remodeling. We recently demonstrated statins induces apoptosis autophagy in human lung mesenchymal cells. Here, we extend our knowledge showing simultaneously activation of the apoptosis, unfolded protein response (UPR) primary atrial fibroblasts (hATF). Thus tested degree to...
Idiopathic pulmonary fibrosis (IPF) is a lethal fibrotic lung disease in adults with limited treatment options. Autophagy and the unfolded protein response (UPR), fundamental processes induced by cell stress, are dysregulated fibroblasts epithelial cells from humans IPF. Human primary cultured parenchymal airway non-IPF IPF donors were stimulated transforming growth factor-β1 (TGF-β1) or without inhibitors of autophagy UPR (IRE1 inhibitor). Using immunoblotting, we monitored temporal changes...
Temozolomide (TMZ) is a chemotherapy agent used to treat Grade IV astrocytoma, also known as glioblastoma (GBM). TMZ treatment causes DNA damage that results in tumor cell apoptosis and increases the survival rate of GBM patients. However, chemoresistance result TMZ‐induced autophagy significantly reduces this anticancer effects over time. Statins are competitive inhibitors HMG‐CoA reductase, rate‐limiting enzyme mevalonate (MEV) cascade. best for their cholesterol (CH)‐lowering effect....
Abstract In this study, we used nitrogen-doped titanium dioxide (N-TiO 2 ) NPs in conjugation with visible light, and show that both reactive oxygen species (ROS) autophagy are induced by novel NP-based photodynamic therapy (PDT) system. While well-dispersed N-TiO (≤100 μg/ml) were inert, their photo-activation light led to ROS-mediated leukemia K562 cells normal peripheral lymphocytes, increased parallel increasing NP concentrations doses. At a constant energy (12 J/cm ), ROS levels trigger...
Autophagy and apoptosis are two major interconnected host cell responses to viral infection, including influenza A virus (IAV). Thus, delineating these events could facilitate the development of better treatment options provide an effective anti-viral strategy for controlling IAV infection. We used A549 cells mouse embryonic fibroblasts (MEF) study role virus-induced autophagy apoptosis, cross-talk between both pathways, their relation infection [ATCC strain A/Puerto Rico/8/34(H1N1)...
During spermiogenesis, the histone-to-protamine replacement causes compaction of spermatid chromatin. The genes for protamines, PRM-1 and PRM-2, are transcribed in round elongating spermatids. transcripts stored a translationally-repressed state by binding protein repressors before being translated elongated RNA extracts from homogenized whole testis samples supply only average data, cell-specific stage-specific expression cannot be addressed. Therefore, we used UV-laser-assisted...
Statins inhibit the proximal steps of cholesterol biosynthesis, and are linked to health benefits in various conditions, including cancer lung disease. We have previously investigated apoptotic pathways triggered by statins airway mesenchymal cells, identified reduced prenylation small GTPases as a primary effector mechanism leading p53-mediated cell death. Here, we extend our studies statin-induced death assessing endpoints both apoptosis autophagy, investigating their interplay coincident...
Subcellular trafficking within host cells plays a critical role in viral life cycles, including influenza A virus (IAV). Thus targeting relevant subcellular compartments holds promise for effective intervention to control the impact of infection. Bafilomycin A1 (Baf-A1), when used at relative high concentrations (≥10 nM), inhibits vacuolar ATPase (V-ATPase) and reduces endosome acidification lysosome number, thus inhibiting IAV replication but promoting cell cytotoxicity. We tested...