A5028011174- Chronic Obstructive Pulmonary Disease (COPD) Research
- Extracellular vesicles in disease
- Respiratory Support and Mechanisms
- Pulmonary Hypertension Research and Treatments
- Blood properties and coagulation
- Viral Infections and Vectors
- Inflammatory Biomarkers in Disease Prognosis
- Air Quality and Health Impacts
- MicroRNA in disease regulation
- Pediatric health and respiratory diseases
- COVID-19 Clinical Research Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cardiac Fibrosis and Remodeling
- Health and Wellbeing Research
- Circular RNAs in diseases
- Immune cells in cancer
- Pituitary Gland Disorders and Treatments
- Myasthenia Gravis and Thymoma
- Cardiovascular Disease and Adiposity
- Pancreatitis Pathology and Treatment
- Cell Adhesion Molecules Research
- Biomarkers in Disease Mechanisms
- Adrenal and Paraganglionic Tumors
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
University of Padua
2020-2024
Abstract The rising incidence of non-ST-segment elevation myocardial infarction (NSTEMI) and associated long-term high mortality constitutes an urgent clinical issue. Unfortunately, the study possible interventions to treat this pathology lacks a reproducible pre-clinical model. Indeed, currently adopted small large animal models MI mimic only full-thickness, ST-segment-elevation (STEMI) infarcts, hence cater for investigation into therapeutics directed at subset MI. Thus, we develop ovine...
Cell-derived extracellular vesicles (EVs) found in the circulation and body fluids contain biomolecules that could be used as biomarkers for lung other diseases. EVs from bronchoalveolar lavage (BAL) might more informative of abnormalities than blood, where information diluted. To compare EVs’ characteristics BAL blood smokers with without COPD. Same-day samples were obtained 9 nonsmokers (NS), 11 w/o COPD (S), (SCOPD) (FEV1: 59 ± 3% pred). After differential centrifugation, (200–500 nm...
The local, extravascular, activation of the coagulation system in response to injury is a key factor mediating resulting inflammatory response. Coagulation Factor XIIIA (FXIIIA) found alveolar macrophages (AM) and dendritic cells (DC), by influencing fibrin stability, might be an modifier COPD.To study expression FXIIIA AM Langerin+DC (DC-1) their relation disease progression COPD.In 47 surgical lungs, 36 from smokers (22 COPD 14 no-COPD) 11 non-smokers we quantified immunohistochemistry...
Microvesicles (MVs) released from almost all cells are recognized as cell communication tools. MVs have been investigated in several inflammatory diseases but poorly biological fluids like bronchoalveolar lavage (BAL) of smokers. The purpose this study was to investigate the presence and source BAL smokers with without chronic obstructive pulmonary disease (COPD) compared nonsmoking controls. Using flow cytometry BAL, we detected endothelial alveolar macrophage (AM)-derived found a higher...
COPD is a major health problem, mainly due to cigarette smoking. Most studies in are dedicated fully developed older subjects, even though development of may start soon after smoking initiation. Therefore, there need diagnose this "early disease" by detecting the initial events responsible for ultimate COPD.Measurement maximum mid expiratory flow between 25 and 75% vital capacity (MMEF) routine spirometry, which detects small airways disease, was used investigate if MMEF abnormalities...
Background: Some 20% of patients with stable Chronic Obstructive Pulmonary Disease (COPD) might have heart failure (HF). HF contribution to acute exacerbations COPD (AECOPD) presenting the emergency department (ED) is not well established. Aims: To assess (1) incidence in ED AECOPD; (2) concordance between and respiratory ward (RW) diagnosis; (3) factors associated risk death after hospital discharge. Methods: Retrospective chart review 119 for exacerbation symptoms then admitted RW where a...
Smokers with and without chronic obstructive pulmonary disease (COPD) are at risk of severe outcomes like exacerbations, cancer, respiratory failure, decreased survival. The mechanisms for these unclear; however, there is evidence that blood lymphocytes (BL) number might play a role.The objective this study to investigate the relationship between BL their possible decline over time long-term in smokers COPD.In 511 smokers, 302 COPD 209 (noCOPD), followed long term, we investigated whether be...
Background The family of Suppressor Cytokine Signaling (SOCS) acts as a controller the duration and intensity cytokine function by negatively regulating JAK-STAT signaling pathway. SOCS’ role in inflammatory diseases animal models is well demonstrated. However, its development human disease still under investigation. SOCS3 plays an important tumor where downregulation has been implicated pathogenesis various solid tumors such triple-negative breast cancer. Aim aim this work was to study (1)...
Depending on local cues, macrophages can polarize into classically activated (M1) or alternatively (M2) phenotypes. This study investigates the impact of polarized macrophage-derived Extracellular Vesicles (EVs) (M1 and M2) their cargo miRNA-19a-3p miRNA-425-5p TGF-β production in lung fibroblasts. EVs were isolated from supernatants M0, M1, M2 quantified using nanoscale flow cytometry prior to fibroblast stimulation. The concentration was measured ELISA assays. expression levels assessed...
The impact that COVID-19 could have on patients with COPD is a real concern. In this study we evaluated, in cohort of longitudinally followed subjects, the incidence COVID-19, seeking for possible risk factors and prognostic predicting clinical outcome. our 370 (followed 5.3 ± 2.7 years), 22 developed (COPD/COVID-19+) between February/November 2020 (5.9%). Cardio-metabolic conditions (hypertension, dyslipidemia, obesity, diabetes) but not respiratory abnormalities (FEV 1 , DLCO, emphysema...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)
Chronic bronchitis (CB) importantly affects outcomes in smokers with COPD, but the effects on without COPD are less well known and emphasized. The aim of our study was to investigate possible CB clinical (noCOPD) compare them (COPD). For that purpose, we studied 511 smokers, 302 209 followed for 10 years an academic ambulatory setting. defined as presence cough sputum production at least 3 months each two consecutive years. All subjects underwent functional examination spirometry, diffusion...
<b>Background:</b> We have previously shown that coagulation Factor XIII (FXIII), an immune modulator, was related to the expression of receptor CXCR3 and CD8+ Tcell inflammation in COPD lungs. Possibly FXIII might participate activation T lymphocytes by recruitment maturation dendritic cells sites lung COPD. <b>Aim:</b> To investigate if inflammatory role could be mediated inflammation. <b>Methods:</b> In Lung tissue from 20 smokers with (COPD) [(FEV<sub>1</sub>: 51 ± % pred; 10 severe...
<b>Background:</b> Cell derived extracellular vesicles (EVs) found in the circulation and body fluids contain biomolecules that could be used as biomarkers for lung other diseases. EVs from BAL might more informative of abnormalities than blood, where information diluted. <b>Aim:</b> To compare characteristics blood smokers with without COPD. <b>Methods:</b> Same day samples were obtained 5 nonsmokers (NS), 11 w/o COPD (S) 9 (SCOPD) (FEV1: 63±3% pred). After differential centrifugation,...
<b>Background:</b> Increasing evidence suggests that an immune dysregulation may be involved in the progression of IPF. The presence lymphoid follicles (LF) (sites where response is triggered) earlyIPF and their evolution with disease, have never been caracterized as part natural history <b>Aim:</b> 1) To investigate LF earlyIPF(EIPF) end-stage late IPF(LIPF) 2) quantify numbers, dimensions state activation from EIPF to LIPF. <b>Methods:</b> immunostained for B-lymphocytes were counted...
<b>Introduction:</b> Originally described as an anti-inflammatory <i>in vitro</i>, the Suppressor of Cytokine Signalling (SOCS) proteins seem to have opposite effect vivo</i>. Along with this possibility we previously observed a higher expression SOCS3 in alveolar macrophages (AM) COPD lungs than healthy smoker and nonsmoker lungs. <b>Aim:</b> To investigate: 1) if AM-derived MV BAL could reflect lung AM; 2) AM is regulated by post-transcriptional regulators, miR-19a-3p miR-221-3p MV....