A5065276549- Gut microbiota and health
- Tryptophan and brain disorders
- Epigenetics and DNA Methylation
- Speech Recognition and Synthesis
- Natural Language Processing Techniques
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Clostridium difficile and Clostridium perfringens research
- Renal and related cancers
- Speech and Audio Processing
- Respiratory viral infections research
- Neuroendocrine regulation and behavior
- RNA Interference and Gene Delivery
- Immune cells in cancer
- IL-33, ST2, and ILC Pathways
- Diet and metabolism studies
- Cancer Immunotherapy and Biomarkers
- Reproductive tract infections research
- Cancer, Stress, Anesthesia, and Immune Response
- Infant Nutrition and Health
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Pregnancy and Medication Impact
- Congenital Diaphragmatic Hernia Studies
- COVID-19 Impact on Reproduction
Cornell University
2017-2024
Weill Cornell Medicine
2022-2024
Icahn School of Medicine at Mount Sinai
2024
New York Proton Center
2022
Memorial Sloan Kettering Cancer Center
2017-2019
The gut microbiota promotes immune system development in early life, but the interactions between metabolome and cells neonatal remain largely undefined. Here, we demonstrate that is uniquely enriched with neurotransmitters, including serotonin, specific bacteria directly produce serotonin while down-regulating monoamine oxidase A to limit breakdown. We found signals T increase intracellular indole-3-acetaldehdye inhibit mTOR activation, thereby promoting differentiation of regulatory cells,...
The gut microbiome elicits antigen-specific immunoglobulin G (IgG) at steady state that cross-reacts to pathogens confer protection against systemic infection. role of microbiome–specific IgG antibodies in the development and immunity enteric early life, however, remains largely undefined. In this study, we show microbiome–induced maternal is transferred neonatal intestine through milk via Fc receptor directly inhibits Citrobacter rodentium colonization attachment mucosa. Enhanced oral C....
The gut microbiome is intricately coupled with immune regulation and metabolism, but its role in Coronavirus Disease 2019 (COVID-19) not fully understood. Severe fatal COVID-19 characterized by poor anti-viral immunity hypercoagulation, particularly males. Here, we define multiple pathways which the protects mammalian hosts from SARS-CoV-2 intranasal infection, both locally systemically, via production of short-chain fatty acids (SCFAs). SCFAs reduced viral burdens airways intestines...
We discovered that induced pluripotent stem cell (iPSC) clones generated from aged tissue donors (A-iPSCs) fail to suppress oxidative phosphorylation. Compared embryonic cells (ESCs) and iPSCs young (Y-iPSCs), A-iPSCs show poor expression of the cell-specific glucose transporter 3 (GLUT3) impaired uptake, making them unable support high demands glycolysis. Persistent phosphorylation in generates higher levels reactive oxygen species (ROS), which leads excessive elevation glutathione (a...
Summary Mismatch repair deficiency (MMRd), either due to inherited or somatic mutation, is prevalent in colorectal cancer (CRC) and other cancers. While anti-PD-1 therapy utilized both local advanced disease, up 50% of MMRd CRC fail respond. Using animal human models MMRd, we determined that interactions between MHC+ C1Q+ CXCL9+ macrophages TCF+ BHLHE40+ PRF1+ T cell subsets are associated with control tumor growth, during treatment. In contrast, resistance upregulation TIM3, LAG3, TIGIT,...
Here, we present a protocol for the development of mRNA-loaded lipid nanoparticle (LNP) vaccines target antigen sequences interest. We describe key steps required to design and synthesize mRNA constructs, their LNP encapsulation, mouse immunization. then detail quality control assays determine RNA purity, guidelines measure immunogenicity using in vitro reporter systems, technique evaluate antigen-specific T cell responses following
The RNA exosome complex targets AU-rich element (ARE)-containing mRNAs in eukaryotic cells. We identified a transcription factor, ZSCAN10, which binds to the promoters of multiple subunits pluripotent stem cells maintain subunit gene expression. discovered that induced cell clones generated from aged tissue donors (A-iPSC) show poor expression leading expression, and subsequent elevation ARE-containing RNAs, including glutathione peroxidase 2 (Gpx2). Excess GPX2 leads excess...
NK cells are innate lymphocytes critical for surveillance of viruses and tumors, however the mechanisms underlying cell dysfunction in cancer incompletely understood. We assessed effector function from bladder patients found severe derived tumors versus peripheral blood. While both tumor-infiltrating exhibited conserved patterns inhibitory receptor over-expression, this did not explain observed defects tumors. Rather, TME-specific TGF-β metabolic perturbations such as hypoxia directly...
We present INDICVOICES, a dataset of natural and spontaneous speech containing total 7348 hours read (9%), extempore (74%) conversational (17%) audio from 16237 speakers covering 145 Indian districts 22 languages. Of these hours, 1639 have already been transcribed, with median 73 per language. Through this paper, we share our journey capturing the cultural, linguistic demographic diversity India to create one-of-its-kind inclusive representative dataset. More specifically, an open-source...
Abstract The gut microbiome promotes immune system development in early life, but the neonatal metabolome remains undefined. Here, we demonstrate that, distinct from adults, mouse is enriched with neurotransmitters, and specific bacteria produce serotonin directly while downregulating monoamine oxidase A to limit breakdown. Serotonin inhibits mTOR activation promote regulatory T cells suppress cell responses both ex vivo intestine. Oral gavage of into mice leads long-term tolerance toward...
Abstract The gut microbiome plays a critical role in neonatal immune development; early-life perturbations to the have been linked later susceptibility respiratory conditions, including asthma and chronic obstructive pulmonary disease. Preterm infants are at higher risk for adverse outcomes infections such as syncytial virus (RSV). premature is significantly altered, but it remains unclear how this affects lung cell development RSV infection independent of immaturity. In study, we generated...
Abstract The gut microbiota elicits antigen-specific IgG at steady state that cross-reacts to pathogenic Gram-negative Enterobacteriaceae confer protection against systemic infection. role of these antibodies in the development microbiome and immunity enteric pathogens early life, however, remains largely undefined. In this study, we demonstrate microbiota-induced maternal is transferred neonatal through milk via Fc receptor confers critical by inhibiting colonization attachment C. rodentium...
Abstract The gut microbiome in neonates is vastly different from that adults due to dietary changes and maturation. However, the neonatal metabolome, largely shaped by bacteria, remains undefined. Using high-throughput metabolomics, we compared >500 metabolites ileum of specific pathogen-free (SPF) wild-type adult mice. We found most at significantly higher levels were neurotransmitters, including serotonin (5-HT), a neurotransmitter critical regulation motility mood stabilization....