A5053223999- Light effects on plants
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Liquid Crystal Research Advancements
- Adhesion, Friction, and Surface Interactions
- Surfactants and Colloidal Systems
- Material Dynamics and Properties
- Force Microscopy Techniques and Applications
- Advanced Materials and Mechanics
- Mechanical and Optical Resonators
- Sports Dynamics and Biomechanics
- Heat Transfer and Boiling Studies
- Plant Reproductive Biology
- Scientific Measurement and Uncertainty Evaluation
- Fluid Dynamics and Heat Transfer
- Nonlinear Dynamics and Pattern Formation
- Heat Transfer and Optimization
- Spacecraft and Cryogenic Technologies
- Granular flow and fluidized beds
École Normale Supérieure de Lyon
2002-2024
Laboratoire de Physique de l'ENS de Lyon
2002-2024
Centre National de la Recherche Scientifique
2010-2024
Université Claude Bernard Lyon 1
2010-2024
Laboratoire de Physique de l'ENS
2022
Optogenetics enables genome manipulations with high spatiotemporal resolution, opening exciting possibilities for fundamental and applied biological research. Here, we report the development of LiCre, a novel light-inducible Cre recombinase. LiCre is made single flavin-containing protein comprising AsLOV2 photoreceptor domain Avena sativa fused to variant carrying destabilizing mutations in its N-terminal C-terminal domains. can be activated within minutes illumination blue light without...
Abstract Systematic measurements of the surface viscosity several nematic liquid crystals in contact with a polymercaptan layer show that latter scales like bulk rotational . This result is interpreted framework ‘delocalized model’ for viscosity. Keywords: crystalssliding anchoringrotational viscosityrotational viscositypolymercaptan Acknowlegements The authors are grateful to A. Pretrosyan lending them his rubbing machine prepare planar samples and Dequidt fruitful comments. One (V.P.-N.)...
We report on a cellular pattern which spontaneously forms at the surface of thin layer cohesive granular material submitted to in-plane stretching. present simple model in mechanism responsible instability is ``strain softening'' exhibited by humid materials above typical strain. Our analysis indicates that such type should be observed any system presenting negative stress sensitivity strain perturbations.
We report on the dynamical buckling of a spherical shell (a table-tennis ball) impinging in normal incidence rigid surface glass plate). Experimentally, we observe and decipher geometrical characteristics profile contact region along with global metrics such as duration coefficient restitution linear velocity. determine, particular, onset ball instability. find that, just like quasi-statics, buckles when crushing exceeds about twice thickness shell. In addition, for launching conditions...
In order to test the fluctuation-dissipation relation on rheological properties of soft materials, we built an experiment measure thermally excited strain in a sample and compare it classical response external stress. The rheometer is based cylindrical Couette geometry. We use differential interferometry achieve better than 10−10 rad/Hz sensitivity angular position above 0.5 Hz. forcing method, electrostatic interaction capacitor, generates torques comparable that thermal noise. Experiments...
We study the action of a rotating magnetic field on Fréedericksz instability under electric homeotropic nematic sample sandwiched between two parallel electrodes. The liquid crystal (LC) is negative dielectric and anisotropies to electrodes used apply field. show that destabilises above critical voltage depends its angular rotation velocity . relation calculated analytically in synchronous regime, where director rotates at same as These predictions are compared experiment performed with LC...
ABSTRACT Previously, we developed an optogenetic tool made of a single chimeric protein called LiCre that enables the edition specific changes in genome live cells with blue light via DNA recombination between loxP sites (Duplus-Bottin et al., 2021). Here, used vitro and vivo experiments combined kinetic modeling to provide deeper characterization photo-activated LiCre- reaction. We find binds high affinity absence stimulus, this binding is cooperative although not as much for Cre...
The potential of cryogenic helium flows for studying high-Reynolds number turbulence in the laboratory has been recognized a long time and implemented several small-scale hydrodynamic experiments. With its large superconducting particle accelerators detector magnets, CERN, European Laboratory Particle Physics, become major world center cryogenics, with refrigerators having capacities up to 18 kW @ 4.5 K. Combining small fraction these resources expertise three laboratories at forefront...
ABSTRACT Optogenetics enables genome manipulations with high spatiotemporal resolution, opening exciting possibilities for fundamental and applied biological research. Here, we report the development of LiCre, a novel light-inducible Cre recombinase. LiCre is made single flavin-containing protein comprising asLOV2 photoreceptor domain Avena sativa fused to variant carrying destabilizing mutations in its N-terminal C-terminal domains. can be activated within minutes illumination blue light,...