The embryo expresses paternal antigens foreign to the mother, and therefore has been viewed as a natural allograft. Cyclosporin A (CsA) is an immunosuppressant for preventing allograft rejection. Little known, however, about modulating effect of CsA on materno-fetal relationship. In this study, pregnant CBA/J female mice mated with DBA/2 or BALB/c male abortion-prone normal pregnancy matings were administered, respectively, at Day 4 gestation. We demonstrated that administration window...

Sulfur hexafluoride (SF6), the strongest greenhouse gas, has great challenges in degradation because of its stable structure, posing significant environmental concerns. Photocatalysis offers an environmentally friendly, low-energy solution, but fluoride deposition on catalysts reduces their activity, thus limiting large-scale application. To prevent catalyst poisoning, we report a thin-layer graphitic carbon nitride (CN) material loaded with MoOx (CNM) that resists for long-term SF6...

Immune regulation during pregnancy is complex, and thus an optimal therapy for complications always a big challenge to reproductive medicine. Cyclosporin A (CsA), potent immunosuppressant, prevents rejection of allografts by hosts, but little known about the modulating effect CsA on materno-fetal relationship. Here, pregnant CBA/J females mated with DBA/2 males as abortion-prone model were administered day 4.5 gestation, BALB/c established successful control. It was demonstrated that...

Maternal-fetal crosstalk during embryo implantation is complex and regulated by local signaling molecules. Chemokines their receptors are critical components required for the process of pregnancy. This study aimed to explore whether human first-trimester trophoblast cells (TCs) were capable modulating migration invasion decidual epithelial (DECs) via CXCL12/CXCR4/CXCR7 signaling.The expression CXCR4 CXCR7 in DECs was examined immunohistochemistry, immunocytochemistry, immunofluorescence,...

<b><i>Aim:</i></b> The study aimed to assess the effect of in vitro vitrification and maturation on nuclear configuration, cytoplasmic global DNA methylation pattern human germinal vesicle (GV) stage oocytes. <b><i>Methods:</i></b> Human oocytes from infertile women were randomly assigned one 3 groups: (i) metaphase II (MII) matured vivo (vivo-MII, n = 56) as controls; (ii) MII (vitro-MII, 106); (iii) that vitrified at GV stage, warmed...

BACKGROUND: Population-based study of semen quality is rare in literature. METHODS: Healthy men aged 20–60 years from six Chinese provinces were invited to participate the between December 2000 and November 2002. Posters distributed participating counties enroll 200 subjects each province. Medians, percentiles, proportions below lower threshold WHO criteria for parameters calculated. Generalized linear models used examine determinants quality. RESULTS: Semen samples 1191 healthy collected...

The aim of this study was to explore whether the morphology polar bodies (PBs) estimated at 16-18 h after insemination can be used as an additional marker for predicting human embryo quality or pregnancy outcome. data from 355 patients who received standard in vitro fertilization (IVF) intracytoplasmic sperm injection (ICSI) treatment controlled ovarian hyperstimulation were recruited. Normal fertilized 3048 zygotes 382 cycles divided into two groups, PBs intact fragmented, according...

Meiosis, which produces haploid progeny, is critical to ensuring both faithful genome transmission and genetic diversity. Proteasomes play roles at various stages of spermatogenesis, including meiosis, but the underlying mechanisms remain unclear. The atypical proteasomes, contain activator PA200, catalyze acetylation-dependent degradation core histones in elongated spermatids DNA repair somatic cells. We show here that testis-specific proteasome subunit α4s/PSMA8 essential for male...

Abstract Background A rapid development in assisted reproductive technology (ART) has led to a surge its popularity among target couples. However, elucidation on the molecular mechanism and effective solutions for common problem posed by ART, namely transfer failure, is still lacking. The new therapeutic potential of cyclosporin (CsA), typical immunosuppressant widely used treatment rejection after organ transplantation, recurrent pregnancy loss (RPL) patients may inspire some novel failure...

Abstract Background CXCL12(chemokine ligand 12, CXCL12) and its receptors CXCR4 are widely expressed in maternal-fetal interface plays an adjust role materno-fetal dialogue immune tolerance during early pregnancy. This study aimed to evaluate the mechanism of self-derived CXCL12 modulating functions human first-trimester endometrial epithelial cells (EECs) identify potential protein kinase signaling pathways involved CXCL12/CXCR4’s effect on EECs. Methods The expression EECs was measured by...

Lung cancer is a major cause of morbidity and mortality worldwide, therefore identifying biomarkers for the early detection, grading or postoperative follow-up lung clinical significance. In present study, expression tissue (t)-CXCL16 t-CXCR6 was examined in 58 patients with non-small cell (NSCLC) using immunohistochemical staining, serum (s)-CXCL16 levels were detected NSCLC 32 normal volunteers an ELISA. A performed every 4 months between January 2014 2015. Compared volunteers, s-CXCL16...

The aim of the study was to examine clinical value blastocysts derived from mono-pronuclear (1PN) or non-pronuclear (0PN) zygotes with two polar bodies (2PB), which were selected by our criteria. We retrospectively analysed 610 frozen-thawed blastocyst transfer (FET) cycles and corresponding oocyte retrieval 2014 2017. Developmental potential outcomes embryos various numbers pronuclei analysed. Based on more detailed pre-selection settings, blastulation rates 1PN/2PB 0PN/2PB-derived 70.18%...

This study aimed to explore the effects of low-concentration (0.6 ng/mL) granulocyte–macrophage colony-stimulating factor (GM-CSF) supplementation on human embryo quality and pregnancy outcomes in patients with fresh transfer cycles. The data were retrospectively collected from 719 hyperstimulation cycles 631 divided into two groups: GM-CSF ng/mL, n = 399) control (n 320). compared. addition significantly increased available rate (52.0 vs. 48.1%, p < .05). In >38 years, it enhanced cleavage...

Development of an effective system for oocyte-cryopreservation is clinical relevance in reproductive medicine. However, oocyte-preservation not as embryo preservation. In this study, we used a 37°C pre-equilibrium temperature part modified vitrification method human oocyte cryopreservation. The effect the new on spindle configuration, chromosomal arrangement, and mitochondrial distribution was investigated vitro-matured oocytes. A total 101 oocytes were randomly assigned at (37°C Group,...

The purpose of this study was to compare the efficacy different open cryo-carriers: CryoloopTM, CryotopTM, and CryoleafTM, in embryo survival clinical outcome patients with frozen transfer (FET) cycle. We analyzed rate 325 348 FET cycles vitrified CryoloopTM (160 cycles), CryotopTM (105 or CryoleafTM (83 cycles). No significant differences were observed (98.8% vs. 100% 97.7%, p > 0.05), HCG positive (58.8% 63.8% 57.8%, biochemical pregnancy (6.9% 11.4% 9.6%, implantation (33.2% 37.4% 34.1%,...