A5102988693- Plant Parasitism and Resistance
- Plant and animal studies
- Enzyme Production and Characterization
- Peptidase Inhibition and Analysis
- Glaucoma and retinal disorders
- Biochemical and Structural Characterization
- Plant Molecular Biology Research
- Liver Disease Diagnosis and Treatment
- Retinal Imaging and Analysis
- Intracranial Aneurysms: Treatment and Complications
- Vascular Malformations Diagnosis and Treatment
- Plant and Fungal Species Descriptions
- Carbohydrate Chemistry and Synthesis
- Alkaline Phosphatase Research Studies
- Insect-Plant Interactions and Control
- Liver Disease and Transplantation
- Protease and Inhibitor Mechanisms
- Spinal Fractures and Fixation Techniques
- Phytochemicals and Antioxidant Activities
- Retinal Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- Biochemical and Molecular Research
- Identification and Quantification in Food
- Neurosurgical Procedures and Complications
- Corneal surgery and disorders
Teikyo University
2002-2024
Kure Kyosai Hospital
2021-2024
Tokyo Medical and Dental University
1978-2021
Kanagawa University of Human Services
2008-2020
Kyorin University
2020
Mie Chuo Medical Center
2019
Chiba Hospital
2019
Naval Hospital Yokosuka Japan
2019
Tokyo Metropolitan University
1985-2005
University of Wisconsin–Madison
2005
Human subjects drank coffee containing 412 μmol of chlorogenic acids, and plasma urine were collected 0 to 24 h after ingestion analyzed by high-performance liquid chromatography-mass spectrometry. Within 1 h, some the components in reached nanomole peak concentrations (<i>C</i><sub>max</sub>), whereas acid metabolites, including caffeic acid-3-<i>O</i>-sulfate ferulic acid-4-<i>O</i>-sulfate sulfates 3- 4-caffeoylquinic lactones, had higher <i>C</i><sub>max</sub> values. The short time...
Major strigolactones (SLs) produced by rice (Oryza sativa L. cv. Nipponbare) and tobacco (Nicotiana tabacum Michinoku No. 1) were purified their stereochemical structures determined comparing with optically pure synthetic standards for NMR CD data retention times mass fragmentations in ESI-LC/MS GC-MS. SLs from root exudates of plants orobanchol, orobanchyl acetate, ent-2'-epi-5-deoxystrigol. In addition to these SLs, 7-oxoorobanchyl acetate the putative three methoxy-5-deoxystrigol isomers...
One of the germination stimulants for root parasitic weeds produced by maize (Zea mays) was isolated and named methyl zealactonoate (1). Its structure determined to be (2E,3E)-4-((RS)-3,3-dimethyl-2-(3-methylbut-2-en-2-yl)-5-oxotetrahydrofuran-2-yl)-2-((((R)-4-methyl-5-oxo-2,5-dihydrofran-2-yl)oxy)methylene)but-3-enoate using 1D 2D NMR spectroscopy ESI EI-MS spectrometry. Feeding experiments with 13C-carlactone (CL), a biosynthetic intermediate strigolactones, confirmed that 1 is from CL in...
We report a case of spontaneous haemothorax in 19 year old boy with an exostosis the left second rib. It may have been caused by nontraumatic rupture markedly dilated pleural vessels, as result long-standing friction between and pleura. This is first haemothorax, without penetrative injury to pleura or diaphragm, patient hereditary multiple exostosis.
The mechanism of floral scent emission was studied in Petunia axillaris, a plant with diurnal rhythm output. rate each volatile compound oscillated synchrony its endogenous concentration, so that the intensity appeared to be determined by concentrations. composition major volatiles flower tissue and headspace showed characteristic differences. A negative correlation found between boiling points compounds ratio their emitted concentrations, indicating depends directly on compounds. We...
Crystal structures of the serine-carboxyl proteinase from Pseudomonas sp. 101 (PSCP), complexed with a number inhibitors, have been solved and refined at high- to atomic-level resolution. All these inhibitors (tyrostatin, pseudo-tyrostatin, AcIPF, AcIAF, chymostatin, as well previously studied iodotyrostatin pseudo-iodotyrostatin) make covalent bonds active site Ser287 through their aldehyde moieties, while side chains occupy subsites S1−S4 enzyme. The mode binding is almost identical for P1...
ABSTRACT The present study emphasizes the principle of using liver support to restore blood ketone body ratio (acetoacetate/β‐hydroxybutyrate), which reflects redox potential mitochondria and correlates with hepatic energy charge (ATP + 0.5ADP/ATP ADP AMP). Eleven surgical patients grade IV coma were treated by an ex vivo pig or baboon cross‐hemodialysis interposed Cuprophan membrane when their ratios had decreased below 0.4 compared normal above 0.7. Three a standard dialyzer without...
Abstract To investigate the relationships between sensitivity loss in various subfields of central 10° binocular integrated visual field (IVF) and vision-related quality life (VRQoL) 172 patients with advanced glaucoma. Using Random Forest algorithm, which controls for inter-correlations among IVF, we analysed Rasch analysis-derived person ability index (RADPAI), age, best-corrected acuity (BCVA), mean total deviations (mTDs) eight quadrant IVF measured Humphrey Field Analyzer (HFA) 10-2...
Xanthomonas carboxyl proteinase (XCP), isolated from sp. T-22, is the second example of unique proteinases [EC 3.4.23.33] which are insensitive to classical aspartic inhibitor. The gene coding for XCP was cloned, sequenced, and expressed in Escherichia coli. contains an open reading frame 2,481 base pairs encoding a protein 827 amino acid residues with Mr 83,677. synthesized as large precursor consisting three regions: NH2-terminal prepro (N-Prepro) (237 residues); mature (398 a.a....
Tyrostatin, a new carboxyl proteinase inhibitor, was discovered in culture filtrate of an actinomycete. From taxonomical studies, the strain identified and named Kitasatosporia sp. No. 55. The inhibitor extracted with ethylacetate, purified by Sephadex LH-20 silica gel column chromatographies, crystallized from methanol. yield about 100 mg 18.2 1 filtrate. structure tyrostatin N-isovaleryl-tyrosyl-leucyl-tyrosinal. Tyrostatin strongly inhibited pepstatin-insensitive proteinases originating...
40 haemodynamically unstable patients with pelvic injuries were studied to assess the ability of plain radiographs detect haemorrhagic sites. Pelvic and bilateral angiograms reviewed separately for detection sites in both anterior posterior segments. Sensitivity specificity obtained by analysis angiographic findings. Angiography demonstrated arterial injury 106 divisions: 26 right anterior, 22 posterior, 33 left 25 posterior. Sensitivities predicting higher segment (right, 96%; left, 100%)...
Jasmonic acid (JA) and its biologically active form jasmonoyl- L -isoleucine (JA-Ile) regulate defense responses to various environmental stresses developmental processes in plants. JA JA-Ile are synthesized from α-linolenic acids derived membrane lipids via 12-oxo-phytodienoic (OPDA). In the presence of JA-Ile, COI1 receptor physically interacts with JAZ repressors, leading their degradation, resulting transcription JA-responsive genes by MYC factors. Although biosynthesis is conserved...
Journal Article A CLN2-Related and Thermostable Serine-Carboxyl Proteinase, Kumamolysin: Cloning, Expression, Identification of Catalytic Serine Residue Get access Hiroshi Oyama, Oyama *Department Applied Biology, Faculty Textile Science, Kyoto Institute TechnologyMatsugasaki, Sakyo-ku, 606-8585 Search for other works by this author on: Oxford Academic PubMed Google Scholar Takatoshi Hamada, Hamada †Department Microbial Technology, Kumamoto TechnologyIkeda 4-22-1, 860-0082 Shin Ogasawara,...
Cucumber (Cucumis sativus) plants were found to exude at least 12 germination stimulants for root parasitic weeds including 5 known strigolactones, 7-oxoorobanchol, 7-oxoorobanchyl acetate, orobanchol, orobanchyl and 4-deoxyorobanchol. Two novel purified from cucumber exudates their structures determined be 7α- 7β-hydroxyorobanchyl acetate by 1D 2D NMR spectroscopy, ESI- EI-MS spectrometry. The stereochemistry was NOE measurement comparing the CD spectra with those of synthetic standards...
A novel method for monitoring proton−deuteron (H/D) exchange at backbone amides is based on the observation of H/D isotope effects 13C NMR signals from peptide carbonyls. The line shape carbonyl 13Ci signal influenced by differential occupancy two adjacent amides: HNi+1 (β site) and HNi (γ site). At a carbon frequency 75.4 MHz, H → D shifts are about 5−7 Hz β site 2 or less γ site. Because sites additive, time dependence particular resonance can report not only rates individual but also...
ABSTRACT The effect of liver support on the adenylate energy charge (ATP + 0.5ADP)/(ATP ADP AMP) remnant after major hepatic resection was studied in rabbits. present study emphasized principle restoring decreased level by use an ex vivo heterologous cross‐he‐modialysis with interposed Cuprophan membrane. provides cell a very sensitive intracellular control mechanism. Regulatory enzymes from biosynthetic sequences exhibit little activity at low levels charge, and their activities increase...