A5002226702- Reproductive Biology and Fertility
- nanoparticles nucleation surface interactions
- Seed Germination and Physiology
- Microencapsulation and Drying Processes
- Microfluidic and Bio-sensing Technologies
- Magnetic and Electromagnetic Effects
- CAR-T cell therapy research
- Thermodynamic properties of mixtures
- Freezing and Crystallization Processes
- Liver physiology and pathology
- thermodynamics and calorimetric analyses
- Sperm and Testicular Function
- Tissue Engineering and Regenerative Medicine
- Microbial Inactivation Methods
- Crystallization and Solubility Studies
- Material Dynamics and Properties
- Viral Infectious Diseases and Gene Expression in Insects
- Liver Disease and Transplantation
- Spectroscopy and Quantum Chemical Studies
- Ovarian function and disorders
- Phase Change Materials Research
- Liver Disease Diagnosis and Treatment
- Food Drying and Modeling
- Phase Equilibria and Thermodynamics
- Plant tissue culture and regeneration
Fujian Medical University
2025
Sana Biotechnology (United States)
2020-2023
Novo Nordisk (United States)
2023
Massachusetts General Hospital
2016-2022
Harvard University
2016-2022
Shriners Hospitals for Children - Boston
2019-2022
Zymergen (United States)
2021
enEvolv (United States)
2019-2021
Center for Excellence in Education
2018
Medical University of the Americas
2017
Deep eutectic solvents (DESs) have emerged as a set of intrinsically "designer solvents" for many bio-applications such DNA nanotechnology and biocatalysis. However, the high viscosity DESs tends to prevent bioactive components from being incorporated into solvent. Although dilution with water may effectively reduce DES, effect on its cooperative hydrogen-bonding network has not been evaluated systematically. This study conducted series molecular dynamics simulations DES made choline...
In phenylketonuria (PKU) patients, a genetic defect in the enzyme phenylalanine hydroxylase (PAH) leads to elevated systemic (Phe), which can result severe neurological impairment. As treatment for PKU, Escherichia coli Nissle (EcN) strain SYNB1618 was developed under Synlogic's Synthetic Biotic™ platform degrade Phe from within gastrointestinal (GI) tract. This clinical-stage engineered expresses Phe-metabolizing ammonia lyase (PAL), catalyzing deamination of non-toxic product...
The state of intracellular water is important in all phases cryopreservation. Intracellular can be transported out the cell, transferred into its solid phase, or blocked by cryoprotectants and proteins cytoplasm. purpose present study to determine amount hydrogen-bonded aqueous ethylene glycol glycerol solutions. effects temperature concentration on density hydrogen bonding characteristics solution are evaluated quantitatively this study. To achieve these aims, a series molecular dynamics...
Ice formation is a ubiquitous process that poses serious challenges for many areas. Nature has evolved variety of different mechanisms to regulate ice formation. For example, cold-adapted species produce antifreeze proteins (AFPs) and/or glycoproteins (AFGPs) inhibit recrystallization. Although several synthetic substitutes AF(G)Ps have been developed, the fundamental principles designing AF(G)P mimics are still missing. In this study, we explored molecular dynamics recrystallization...
Molecular dynamics simulations have been performed to investigate the aqueous binary mixtures of alcohols, including methanol, ethylene glycol (EG) and glycerol molalities ranging from 1 5 m at temperatures 273, 288 298 K, respectively. The primary purpose this paper is mechanism water self-diffusion in water-alcohol point view hydrogen bonding. effects temperature concentration on coefficient are evaluated quantitatively work. Temperature some extent affect bonding statistics mixtures. It...
A passive pumping, single-cell trapping microarray was developed to monitor volumetric change of multiple, single cells following hypertonic exposure.
Human infertility can be treated using assisted reproductive technology (ART) such as intracytoplasmic sperm injection (ICSI). But current ART techniques suffer from multiple cumbersome processes requiring technically skilled personnel. Microfluidics technologies offer unique opportunities to streamline procedures, reduce stress imposed upon gametes and embryos, minimize the operator-to-operator variability. However, there have been no automated continuous processing systems that dependence...
Ice nucleation is of fundamental significance in many areas, including atmospheric science, food technology, and cryobiology. In this study, we investigated the ice-nucleation characteristics picoliter-sized drops consisting different D2O H2O mixtures with without ice-nucleating bacteria Pseudomonas syringae. We also studied effects commonly used cryoprotectants such as ethylene glycol, propylene trehalose on mixtures. The results show that median freezing temperature suspension containing 1...
Loss of hepatocyte viability and metabolic function after cryopreservation is still a major issue. Although vitrification promising alternative, it has generally been proven to be unsuitable for large cell volumes which required clinical applications. Here, we propose novel bulk droplet (3–5 mm diameter) method allows high throughput (4 mL/min), while using low preincubated CPA concentration (15% v/v) minimize toxicity loss function. We used rapid (1.25 s) osmotic dehydration concentrate...
Abstract Dry preservation of biologics in sugar glasses is regarded as a promising alternative to conventional cryopreservation. Evidence from various studies has suggested that there critical range water content beyond which the viability preserved can be greatly compromised. In this study T-cells was determined function end after microwave-assisted drying trehalose solutions. Hydrogen-bonding and clustering phenomena solutions same moisture were also evaluated using molecular dynamics...
<italic>T</italic><sub>g</sub> behavior of trehalose–water mixtures is reproduced by molecular modeling with insights into the dynamic/thermodynamic characteristics associated vitrification.
Cryopreservation is of significance in areas including tissue engineering, regenerative medicine, and organ transplantation. We investigated endothelial cell attachment membrane integrity a microvasculature model at high subzero temperatures the presence extracellular ice. The results show that heterogeneous ice formation induced by nucleating bacteria, cells showed improved temperature minimums -6 °C. However, as decreased below °C, required additional cryoprotectants. glucose analog,...
Dimethyl sulfoxide is one of the most popular cryoprotectants in cryobiology. It crucial for successful cryopreservation to quantitatively analyze cryoprotective properties dimethyl sulfoxide. In this study, osmolality and unfrozen water content aqueous solution are determined using a Perkin-Elmer Diamond DSC. For precisely calculating content, study takes into consideration effect dilution on enthalpy fusion which commonly neglected factor. The osmotic virial representation quantitative...
Hydrogen bonding interaction between alcohols and water molecules is an important characteristic in the aqueous solutions of alcohols. In this paper, a series molecular dynamics simulations have been performed to investigate low weight (methanol, ethylene glycol glycerol) at concentrations covering broad range from 1 90 mol %. The work focuses on studying effect hydrogen binary mixtures. By analyzing ability hydroxyl (-OH) groups for three alcohols, it found that group methanol prefers form...
Approximately a decade ago it was observed that adding small amount (5 wt %) of glycerol to trehalose could substantially improve the stability enzymes stored in these glasses even though final glass transition temperature (Tg) reduced by ∼20 K. This finding inspired great interest fast dynamics dehydrated trehalose/glycerol mixtures, leading observation suppression optimal presence ∼5 % glycerol. It also recognized should, theory, be related fragility formers, but experimental confirmation...