A5089711214- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Circadian rhythm and melatonin
- Light effects on plants
- Virus-based gene therapy research
- Plant Molecular Biology Research
- Protein Structure and Dynamics
- Photoreceptor and optogenetics research
- Genetics, Aging, and Longevity in Model Organisms
- Peroxisome Proliferator-Activated Receptors
- Enzyme Structure and Function
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Neuroscience of respiration and sleep
- RNA modifications and cancer
- Cancer therapeutics and mechanisms
- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Algal biology and biofuel production
- Plant Disease Resistance and Genetics
- Cancer-related molecular mechanisms research
- Heat shock proteins research
- Spaceflight effects on biology
- Photosynthetic Processes and Mechanisms
University of Gothenburg
2020-2023
Charité - Universitätsmedizin Berlin
2006-2021
Humboldt-Universität zu Berlin
2020-2021
Freie Universität Berlin
2020-2021
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2020
University of Bayreuth
2001-2002
PERIOD (PER) proteins are central components within the mammalian circadian oscillator, and believed to form a negative feedback complex that inhibits their own transcription at particular phase. Phosphorylation of PER regulates stability as well subcellular localization. In systematic screen, we have identified 21 phosphorylated residues mPER2 including Ser 659, which is mutated in patients suffering from familial advanced sleep phase syndrome (FASPS). When expressing FASPS-mutated...
Post-translational processes are essential for the generation and dynamics of mammalian circadian rhythms. In particular, phosphorylation key protein PER2 precisely controls period phase oscillations. However, mechanisms underlying that control poorly understood. Here, we identified in a high-throughput RNAi-based genetic screen casein kinase 2 (CK2) as PER2-phosphorylating novel component clock. When CK2 subunits silenced by RNAi or when activity is inhibited pharmacologically, rhythms...
Regulated degradation of circadian clock proteins is a crucial step for rhythm generation per se but also establishing normal period. Here, the authors show that F-box protein beta-transducin repeat containing 1 (beta-TrCP1) as part E3 ubiquitin ligase complex an essential component mammalian oscillator. Down-regulation endogenous beta-TrCP1 well expression dominant-negative form both result in lengthening period oscillating fibroblasts. These phenotypes are due to impaired PERIOD (PER)...
Circadian rhythms are essential to the temporal regulation of molecular processes in living systems and as such life itself. Deregulation these leads failures biological eventually manifestation pathological phenotypes including cancer. To address questions what elicitors a disrupted clock cancer, we applied biology approach correlate experimental, bioinformatics modelling data from several cell line models for colorectal skin We found strong weak circadian oscillators within same type...
Abstract The cell biology of circadian clocks is still in its infancy. Here, we describe an efficient strategy for generating knock-in reporter lines using CRISPR technology that particularly useful genes expressed transiently or at low levels, such as those coding clock proteins. We generated single and double cells with endogenously PER2 CRY1 fused to fluorescent proteins allowing us simultaneously monitor the dynamics live cells. Both are highly rhythmic nucleus human showing a much...
Abstract Neuroblastoma has a low mutation rate for the p53 gene. Alternative ways of inactivation have been proposed in neuroblastoma, such as abnormal cytoplasmic accumulation wild-type p53. However, mechanisms leading to via are not well investigated. Here we show that neuroblastoma risk-associated locus 6p22.3-derived tumor suppressor NBAT1 is p53-responsive lncRNA regulates subcellular levels. Low expression provided resistance genotoxic drugs by promoting cytoplasm and loss from...
The hrcA gene of Bacillus subtilis codes for a transcriptional repressor protein that negatively regulates expression the heptacistronic dnaKand bicistronic groE operon by binding to an operator-element called CIRCE. Recently, we have published data suggesting activity HrcA is modulated GroE chaperonin system. Biochemical analyses been hampered so far its strong tendency aggregate. Here, genetic method was used isolate mutant forms with increased under conditions decreased function. One...
In mammals, molecular circadian rhythms are generated by autoregulatory transcriptional-translational feedback loops with PERIOD/CRYPTOCHROME containing complexes inhibiting the transcription of their own genes. Although major oscillator components seem to be identified, an increasing number additional factors modulating core clock component functions being discovered. a systematic screen using short hairpin RNA in human reporter cells, we identified FBXL11 (also known as KDM2A),...
Abstract Despite the rapid improvements in unveiling importance of lncRNAs all aspects cancer biology, there is still a void mechanistic understanding their role DNA damage response. Here we explored potential oncogenic lncRNA SCAT7 (ELF3-AS1) maintenance genome integrity. We show that upregulated response to DNA-damaging drugs like cisplatin and camptothecin, where expression required promote cell survival. silencing leads decreased proliferation cisplatin-resistant cells vitro vivo through...
In silico analysis of the complete Bacillus subtilis genome revealed presence three genes whose deduced amino acid sequences exhibit an α-crystallin domain characteristic for family small heat shock proteins: cotM (which has already been identified [Henriques et al. (1997) J. Bacteriol 179, 1887–1897]), yocM, and cotP (formerly ydfT). Analysis expression all by slot-blot experiments transcriptional fusions that none them was heat-inducible. Transcription induced late during sporulation...
ABSTRACT The current model of the mammalian circadian oscillator is predominantly based on data from genetics and biochemistry experiments, while cell biology clocks still in its infancy. Here, we describe a new strategy for efficient generation knock-in reporter lines using CRISPR technology that particularly useful lowly or transiently expressed genes, such as those coding clock proteins. We generated single double cells with endogenously PER2 CRY1 fused to fluorescent proteins, which...
<p>Differentially expressed genes in SH-SY5Y neuroblastoma cells after treatment with nutlin-3a, as detected by RNA-seq.</p>
<div>Abstract<p>Neuroblastoma has a low mutation rate for the <i>p53</i> gene. Alternative ways of p53 inactivation have been proposed in neuroblastoma, such as abnormal cytoplasmic accumulation wild-type p53. However, mechanisms leading to via are not well investigated. Here we show that neuroblastoma risk-associated locus 6p22.3-derived tumor suppressor <i>NBAT1</i> is p53-responsive lncRNA regulates subcellular levels. Low expression provided resistance...
<p>Supplementary Text, Supplementary Table Legends</p>
<p>Supplementary Figures S1-S6</p>
<p>Supplementary Text, Supplementary Table Legends</p>
<p>Primers used in the current investigation.</p>
<p>GSEA enriched pathways and list of p53 pathway genes in nutlin-3a treated control NBAT1 depleted SH-SY5Y cells detected by RNA-seq.</p>
<div>Abstract<p>Neuroblastoma has a low mutation rate for the <i>p53</i> gene. Alternative ways of p53 inactivation have been proposed in neuroblastoma, such as abnormal cytoplasmic accumulation wild-type p53. However, mechanisms leading to via are not well investigated. Here we show that neuroblastoma risk-associated locus 6p22.3-derived tumor suppressor <i>NBAT1</i> is p53-responsive lncRNA regulates subcellular levels. Low expression provided resistance...
<p>Differentially expressed genes in SH-SY5Y neuroblastoma cells after treatment with nutlin-3a, as detected by RNA-seq.</p>