A5030321978- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA Research and Splicing
- RNA modifications and cancer
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Signaling Pathways in Disease
- Cell death mechanisms and regulation
- Cancer Mechanisms and Therapy
- Lipid metabolism and disorders
- Lung Cancer Research Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Phagocytosis and Immune Regulation
- Genomics and Chromatin Dynamics
- Plant Disease Resistance and Genetics
- Ocular Oncology and Treatments
- Genomics, phytochemicals, and oxidative stress
- Pancreatic function and diabetes
- Cancer therapeutics and mechanisms
- Peptidase Inhibition and Analysis
- Metabolism, Diabetes, and Cancer
- Acute Myeloid Leukemia Research
- Neuroblastoma Research and Treatments
Universidad de Navarra
2020-2024
Navarre Institute of Health Research
2023-2024
Clinica Universidad de Navarra
2023
University of Turin
2022
University of Gothenburg
2015-2021
University of Catania
2009-2015
The RNA that is packaged into exosomes termed as exosomal-shuttle (esRNA); however, the players, which take this subset of (esRNA) exosomes, remain largely unknown. We hypothesized binding proteins (RBPs) could serve key players in mechanism, by making complexes with RNAs and transporting them during biosynthesis exosomes. Here, we demonstrate presence 30 RBPs were shown to form RNA–RBP both cellular exosomal-RNA species. To assess involvement these RNA-transfer gene transcripts encoding six...
Uveal melanoma (UM) represents approximately 5–6% of all diagnoses and up to 50% patients succumb their disease. Although several methods are available, accurate diagnosis is not always easily feasible because potential accidents (e.g., intraocular hemorrhage). Based on the assumption that profile circulating miRNAs often altered in human cancers, we verified whether UM showed different vitreous humor (VH) or serum miRNA profiles with respect healthy controls. By using TaqMan Low Density...
Despite improvement in our understanding of long noncoding RNAs (lncRNAs) role cancer, efforts to find clinically relevant cancer-associated lncRNAs are still lacking. Here, using nascent RNA capture sequencing, we identify 1145 temporally expressed S-phase-enriched lncRNAs. Among these, 570 show significant differential expression at least one tumor type across TCGA data sets. Systematic clinical investigation 14 Pan-Cancer sets identified 633 independent prognostic markers. Silencing the...
The relationship between therapeutic response and modifications of microRNA (miRNA) transcriptome in colorectal cancer (CRC) remains unknown. We investigated this issue by profiling the expression 667 miRNAs 2 human CRC cell lines, one sensitive other resistant to cetuximab (Caco-2 HCT-116, respectively), through TaqMan real-time PCR. Caco-2 HCT-116 expressed different sets after treatment. Specifically, 21 22 were differentially or respectively (t test, P < 0.01). By testing patients, we...
The molecular bases of mammalian pancreatic α cells higher resistance than β to proinflammatory cytokines are very poorly defined. MicroRNAs master regulators cell networks, but only scanty data available on their transcriptome in these and its alterations diabetes mellitus.Through high-throughput real-time PCR, we analyzed the steady state microRNA murine (αTC1-6) (βTC1) cells: comparison demonstrated significant differences. We also characterized αTC1-6 after treatment with cytokines....
Exchange of molecules via exosomes is a means eukaryotic intercellular communication, especially within tumour microenvironments. However, no data are available on alterations exosomal molecular cargo by environmental cues (eg, pharmacological treatments). To approach this issue, we compared the abundance 754 miRNAs and 741 cancer-related proteins in secreted Caco-2 (Cetuximab-responsive) HCT- 116 (Cetuximab-resistant) CRC cells, before after Cetuximab treatment, with that their source...
Abstract Cells must coordinate the activation of thousands replication origins dispersed throughout their genome. Active transcription is known to favor formation mammalian origins, although role that RNA plays in this process remains unclear. We show ORC1 subunit human Origin Recognition Complex interacts with RNAs transcribed from genes start sites (TSSs), displaying a positive correlation between binding and origin activity. depletion, or use RNA-binding mutant, result inefficient...
ABSTRACT Besides the well-characterized protein network involved in replication stress response, several regulatory RNAs have been shown to play a role this critical process. However, it has remained elusive whether they act locally at stressed forks. Here, by investigating localizing on chromatin upon induced hydroxyurea, we identified set of lncRNAs upregulated S-phase and controlled transcription factors. Among them, demonstrate that previously uncharacterized lncRNA lncREST (long...
Abstract Cells have evolved a robust and highly regulated DNA damage response to preserve their genomic integrity. Although increasing evidence highlights the relevance of RNA regulation, our understanding its impact on fully efficient remains limited. Here, through targeted CRISPR-knockout screen, we identify RNA-binding proteins modifiers that participate in p53 response. Among top hits, find m 6 A reader YTHDC1 as master regulator expression. binds transcription start sites TP53 other...
According to the different sensitivity of their bone marrow CD34+ cells in vitro treatment with Etoposide or Mafosfamide, Acute Myeloid Leukaemia (AML) patients apparent complete remission (CR) after chemotherapy induction may be classified into three groups: (i) normally responsive; (ii) chemoresistant; (iii) highly chemosensitive. This inversely correlates vivo mobilization and, interestingly, also prognosis disease: showing a good mobilizing activity are resistant and subject...
Apoptosis is a critical biological phenomenon, executed under the guidance of Apoptotic Machinery (AM), which allows physiologic elimination terminally differentiated, senescent or diseased cells. Because its relevance to BioMedicine, we have sought obtain detailed characterization AM Omics in Homo sapiens, namely Genomics and Evolution, Transcriptomics, Proteomics, Interactomics, Oncogenomics, Pharmacogenomics.This project exploited methodology commonly used Computational Biology (i.e.,...
Abstract Despite the rapid improvements in unveiling importance of lncRNAs all aspects cancer biology, there is still a void mechanistic understanding their role DNA damage response. Here we explored potential oncogenic lncRNA SCAT7 (ELF3-AS1) maintenance genome integrity. We show that upregulated response to DNA-damaging drugs like cisplatin and camptothecin, where expression required promote cell survival. silencing leads decreased proliferation cisplatin-resistant cells vitro vivo through...
Cells have evolved a robust and highly regulated DNA damage response to preserve their genomic integrity. Although increasing evidence highlights the relevance of RNA regulation, our understanding its impact on fully efficient remains limited. Here, through targeted CRISPR-knockout screen, we identified binding proteins modifiers that participate in mediating p53 response. Among top hits, m6A reader YTHDC1 was as master regulator expression. binds transcription start sites TP53 other genes...
Abstract Cells must coordinate the activation of thousands replication origins dispersed throughout their genome. Active transcription is known to favor formation mammalian origins, although role that RNA plays in this process remains unclear. We show ORC1 subunit human Origin Recognition Complex interacts with RNAs transcribed from genes start sites (TSSs), displaying a positive correlation between binding and origin activity. depletion, or use RNA-binding mutant, result inefficient...
Supplementary Data Legends from Specific Alterations of MicroRNA Transcriptome and Global Network Structure in Colorectal Carcinoma after Cetuximab Treatment
Supplementary Data 5 from Specific Alterations of MicroRNA Transcriptome and Global Network Structure in Colorectal Carcinoma after Cetuximab Treatment
Supplementary Data 6 from Specific Alterations of MicroRNA Transcriptome and Global Network Structure in Colorectal Carcinoma after Cetuximab Treatment