A5010634314- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Extracellular vesicles in disease
- Genetics, Aging, and Longevity in Model Organisms
- Micro and Nano Robotics
- MicroRNA in disease regulation
- Protist diversity and phylogeny
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Neurological diseases and metabolism
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Genetic Syndromes and Imprinting
- Lysosomal Storage Disorders Research
Rutgers, The State University of New Jersey
2018-2023
University of Georgia
2010
Extracellular vesicles (EVs) may mediate intercellular communication by carrying protein and RNA cargo. The composition, biology, roles of EVs in physiology pathology have been primarily studied the context biofluids cultured mammalian cells. experimental tractability C. elegans makes for a powerful vivo animal system to identify study EV cargo from its cellular source. We developed an innovative method label, track, profile using genetically encoded, fluorescent-tagged conducted large-scale...
Cilia both receive and send information, the latter in form of extracellular vesicles (EVs). EVs are nano-communication devices that influence cell, tissue, organism behavior. Mechanisms driving ciliary EV biogenesis almost entirely unknown. Here, we show G-protein Rab28, associated with human autosomal recessive cone-rod dystrophy, negatively regulates levels sensory organs Caenorhabditis elegans a cilia specific manner. Sequential targeting lipidated Rab28 to periciliary membranes is...
The current consensus on cilia development posits that the ciliary transition zone (TZ) is formed via extension of nine centrosomal microtubules. In this model, TZ structure remains unchanged in microtubule number throughout cilium life cycle. This model does not however explain structural variations seen nature and could also lend itself to misinterpretation deviations from nine-doublet ultrastructure represent an abnormal phenotype. Thus, a better understanding events occur at vivo during...
Ciliary microtubules are subject to post-translational modifications that act as a "Tubulin Code" regulate motor traffic, binding proteins and stability. In humans, loss of CCP1, cytosolic carboxypeptidase tubulin deglutamylating enzyme, causes infantile-onset neurodegeneration. C. elegans, mutations in ccpp-1, the homolog result progressive degeneration neuronal cilia function. To identify genes microtubule glutamylation ciliary integrity, we performed forward genetic screen for suppressors...
ABSTRACT Cilia both receive and send information, the latter in form of extracellular vesicles (EVs). EVs are nano-communication devices that cells shed to influence cell, tissue, organism behavior. Mechanisms driving ciliary EV biogenesis environment release almost entirely unknown. Here, we show G-protein RAB28, associated with human autosomal recessive cone-rod dystrophy, negatively regulates levels sensory organs Caenorhabditis elegans . We also find sequential targeting lipidated RAB28...
Abstract Ciliary microtubules are subject to post-translational modifications that act as a “Tubulin Code” regulate motor traffic, binding proteins and stability. In humans, loss of CCP1, c ytosolic arboxy p eptidase tubulin deglutamylating enzyme, causes infantile-onset neurodegeneration. C. elegans, mutations in ccpp-1 , the homolog result progressive degeneration neuronal cilia function. To identify genes microtubule glutamylation ciliary integrity, we performed forward genetic screen for...
Abstract Extracellular vesicle (EV)-based signaling is a challenge to study, due EV small size, heterogeneity, and limited information on cargo content in vivo . We present Caenorhabditis elegans as discovery platform that allows single tracking from source target tissue living animals. enriched ciliary EVs using GFP-tagged PKD-2 followed by mass spectrometry analysis identify 2,888 candidates. By integrating our dataset with single-cell transcriptomic data, we identified produced individual...
Abstract The transition zone (TZ) regulates cilia composition and function. Canonical TZs with 9 doublet microtubules (MTs) are common but non-canonical that vary from MT symmetry also occur arise through unknown mechanisms. Cilia on the quadrant inner labial type 2 (IL2Q) neurons of C. elegans have a specialized TZ fewer than MTs. We previously showed in IL2Q via loss reorganization canonical TZs. Here, we identify structural events mechanisms generate Cell-specific α-tubulin TBA-6...
Abstract Background information The current consensus on cilia development posits that the ciliary transition zone (TZ) is formed via extension of nine centrosomal microtubules. In this model, TZ structure remains unchanged in microtubule number throughout cilium life cycle. This model does not however explain structural variations seen nature, and could also lend itself to misinterpretation deviations from nine-doublet ultrastructure represent an abnormal phenotype. Thus, a better...