Our previous studies demonstrated that transplantation of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) into the hippocampus a transgenic mouse model Alzheimer's disease (AD) reduced amyloid-β (Aβ) plaques and enhanced cognitive function through paracrine action. Due to limited life span hUCB-MSCs after their transplantation, extension hUCB-MSC efficacy was essential for AD treatment. In this study, we show repeated cisterna magna injections activated endogenous...

Abstract Aging is a complex process best characterized as the chronic dysregulation of cellular processes leading to deteriorated tissue and organ function. While aging cannot currently be prevented, its impact on lifespan healthspan in elderly can potentially minimized by interventions that aim return these optimal Recent studies have demonstrated partial reprogramming using Yamanaka factors (or subset; OCT4, SOX2 , KLF4; OSK) reverse age-related changes vitro vivo . However, it still...

Electroacupuncture (EA) is a novel therapy based on traditional acupuncture combined with modern eletrotherapy that currently being investigated as treatment for acute ischemic stroke. Here, we studied whether EA stimulation improves tissue and functional outcome following experimentally induced cerebral ischemia in mice. We hypothesized endothelial nitric oxide synthase (eNOS)-mediated perfusion augmentation was related to the beneficial effects of by interventions injury. at Baihui (GV20)...

Highlights•Induction of Sestrin2 is critical for glutamine-depleted lung cancer cell survival•Glutamine depletion creates a positive feedback loop between and mTORC2•Differential regulation mTORC1 2 by maintains ATP redox balance•Co-targeting SLC1A5 promising therapeutic strategy cancerSummaryProper mTORC2 upon nutrient starvation survival. Upregulation in response to glutamine deprivation rescues death suppressing mTORC1. However, the contribution Sestrin2-mediated suppression remains...

Alzheimer's disease (AD) is characterized by deficits in memory and cognitive functions. The accumulation of amyloid beta peptide (Aβ) oxidative stress the brain are most common causes AD. Caffeic acid (CA) an active phenolic compound that has a variety pharmacological actions. We studied protective abilities CA Aβ25-35-injected AD mouse model. was administered at oral dose 10 or 50 mg/kg/day for 2 weeks. Behavioral tests including T-maze, object recognition, Morris water maze were carried...

The tyrosine kinase inhibitor sorafenib is the only therapeutic agent approved for treatment of advanced hepatocellular carcinoma (HCC), but acquired resistance to high. Here, we report metabolic reprogramming in sorafenib-resistant HCC and identify a regulatory molecule, peroxisome proliferator-activated receptor-δ (PPARδ), as potential target. Sorafenib-resistant cells showed markedly higher glutamine metabolism reductive carboxylation, which was accompanied by increased glucose-derived...

The present study was conducted to examine whether morroniside has an ameliorative effect on diabetes-induced alterations such as oxidative stress, inflammation, and apoptosis in the liver of type 2 diabetic db/db mice. Morroniside (20 or 100 mg/kg body weight/d, per os (p.o.)) administered every day for 8 weeks mice, its compared with vehicle-treated m/m administration decreased elevated serum glucose concentration reduced increased biomarkers including generation reactive oxygen species...

The effects of nattokinase on the in vitro platelet aggregation and vivo thrombosis were investigated comparison with aspirin. Rabbit platelet-rich plasma was incubated inducers collagen thrombin, rate analyzed. Nattokinase significantly inhibited both collagen- thrombin-induced aggregations. also reduced thromboxane B2 formation from collagen-activated platelets a concentration-dependent manner. Rats orally administered for 1 week, their carotid arteries exposed. Arterial induced by...

Hypercholesterolemia is a known risk factor for Alzheimer's disease (AD). In the present study, we investigated whether diet-induced hypercholesterolemia affects AD-like pathologies such as amyloid β-peptide (Aβ) deposition, tau pathology, inflammation and cognitive impairment, using an Aβ25-35-injected pathological mouse model. was induced by providing apolipoprotein E knock out (Apo KO) mice with high-fat diet 4 weeks prior to Aβ25-35 injection following injection, total of 8 treatment....

Background The aim of this study is to determine anti-cancer effect Icariside II purified from the root Epimedium koreanum Nakai on human acute myeloid leukemia (AML) cell line U937. Methodology/Principal Findings blocked growth U937 cells in a dose- and time-dependent manner. In anti-proliferation process, herb compound rendered susceptible apoptosis, manifested by enhanced accumulation sub-G1 population increased terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive...

Ordered lipid domains (rafts) in plasma membranes have been hypothesized to participate endocytosis based on inhibition of by removal or sequestration cholesterol. To more carefully investigate the role sterol endocytosis, we used a substitution strategy replace cholesterol with sterols that show various raft-forming abilities and chemical structures. Both clathrin-mediated transferrin clathrin-independent clustered placental alkaline phosphatase were measured. A subset reversibly inhibited...

Cholinergic dysfunction and oxidative stress are the most common causes of Alzheimer's disease (AD). Safflower seed contains various anti-oxidant cholinergic improvement compounds, such as serotonin its derivatives. In present study, we investigated protective effects mechanisms a safflower extract on scopolamine-induced memory impairment in mouse model. The was orally administered at dose 100 mg kg-1 day-1, then behavior tests (such T-maze novel object recognition tests) were conducted....

This study aims to investigate the protective effects and mechanisms of pectolinarin against oxidative stress-induced cell damage in SH-SY5Y cells. Neurodegenerative diseases-such as Alzheimer's disease-are potentially associated with stress, which causes excessive production reactive oxygen species (ROS) that DNA proteins neuronal The results this demonstrate can scavenge hydroxyl nitric oxide radicals a concentration-dependent manner. Moreover, significantly increased viability while...

We investigated the antidiabetic potential of proanthocyanidin and its oligomeric form in STZ-induced diabetic model rats db/db type 2 mice. Proanthocyanidin ameliorated condition by significant decreases serum glucose, glycosylated protein, urea nitrogen as well urinary protein renal-AGE decrease glucose The suppression ROS generation elevation GSH/GSSG ratio were also observed groups administered proanthocyanidin. Moreover, proanthocyanidin, especially form, affected inflammatory process...

Abstract Background This study was designed to determine if electroacupuncture (EA) preconditioning improves tissue outcome and functional following experimentally induced cerebral ischemia in mice. In addition, we investigated whether the expression of brain-derived neurotrophic factor (BDNF) stromal cell derived factor-1α (SDF-1α) infarct volume were related with improvement neurological motor function by interventions this study. Methods After treatment EA at acupoints ‘Baihui (GV20)’...

We investigated the protective activities of quercetin (Q) and quercetin-3-β-d-glucoside (Q3G) under Aβ25–35-injected Alzheimer's disease (AD) model mice. To induce an AD-like in vivo model, mice were injected with Aβ25–35 via intracerebroventricular route. Q Q3G administered orally for 14 days at 30 mg/kg/day. Learning memory functions evaluated using behavioral experiments that comprise tests like T-maze, Morris water maze, novel object recognition. The administration improved cognitive...