A5101805951- Digital Holography and Microscopy
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Axon Guidance and Neuronal Signaling
- Optical measurement and interference techniques
- Image Processing Techniques and Applications
- Biomarkers in Disease Mechanisms
University of Illinois Urbana-Champaign
2021
University of Illinois Chicago
2016
Primary neuronal cultures have been widely used to study morphology, neurophysiology, neurodegenerative processes, and molecular mechanism of synaptic plasticity underlying learning memory. However, the unique behavioral properties neurons make them challenging study, with phenotypic differences expressed as subtle changes in arborization rather than easy-to-assay features such cell count. The need analyze growth, intracellular transport has motivated development increasingly sophisticated...
Primary neuronal cultures have been widely used to study morphology, neurophysiology, neurodegenerative processes, and molecular mechanism of synaptic plasticity underlying learning memory. Yet, the unique behavioral properties neurons make them challenging - with phenotypic differences expressed as subtle changes in arborization rather than easy assay features such cell count. The need analyze growth, intracellular transport has motivated development increasingly sophisticated microscopes...
In life sciences, fluorescent labeling techniques are used to study molecular structures and interactions of cells. However, this type cell imaging has its own limitations, one which is that the process staining cells could be toxic possibly damage them. We specifically interested in time-lapse live neurons their growth proliferation. Neurodegenerative diseases characterized by phenotypic differences neuron arborization. This thesis proposes a label-free digital method using deep...