A5007530963- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Mitochondrial Function and Pathology
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Genetics and Neurodevelopmental Disorders
- Alzheimer's disease research and treatments
- Cellular transport and secretion
- Memory and Neural Mechanisms
- Receptor Mechanisms and Signaling
- Advanced Electron Microscopy Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Quantum Dots Synthesis And Properties
- Epilepsy research and treatment
- Nanoplatforms for cancer theranostics
- Pharmacological Effects and Toxicity Studies
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
University of Illinois Urbana-Champaign
2015-2024
University of Illinois System
2024
Laboratoire d’immunologie intégrative du cancer
2017-2018
Society for Neuroscience
2017
Urbana University
2016
Ewha Womans University
2016
Catholic Kwandong University
2015
National Health Insurance Service
2014
University of California, San Francisco
2006-2009
Howard Hughes Medical Institute
2000-2006
PSD-95, DLG, ZO-1 (PDZ) domain-mediated protein interactions have been shown to play important roles in the regulation of glutamate receptor function at excitatory synapses. Recent studies demonstrating rapid AMPA during synaptic plasticity suggested that interaction with PDZ domain-containing proteins may be dynamically modulated. Here we show PKC phosphorylation GluR2 subunit differentially modulates its GRIP1 and PICK1. The serine residue [serine-880 (Ser880)] C-terminal sequence (IESVKI)...
Cerebellar LTD requires activation of PKC and is expressed, at least in part, as postsynaptic AMPA receptor internalization. Recently, it was shown that internalization clathrin-mediated endocytosis depends upon the carboxy-terminal region GluR2/3. Phosphorylation Ser-880 this by differentially regulates binding PDZ domain–containing proteins GRIP/ABP PICK1. Peptides, corresponding to phosphorylated dephosphorylated GluR2 motif, were perfused cerebellar Purkinje cells grown culture. Both...
The interaction of PDZ domain-containing proteins with the C termini α-amino-3-hydroxy-5-methylisoxazolepropionate (AMPA) receptors has been suggested to be important in regulation receptor targeting excitatory synapses. Recent studies have shown that rapid internalization AMPA at synapses may mediate, least part, expression long-term depression (LTD). We previously phosphorylation Ser-880 on GluR2 subunit differentially regulated GRIP1 and PICK1. Here, we show induction LTD hippocampal...
Cerebellar long-term depression (LTD) is a model of synaptic memory that requires protein kinase C (PKC) activation and expressed as reduction in the number postsynaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors. LTD was absent cultured cerebellar Purkinje cells from mutant mice lacking AMPA receptor GluR2 subunit could be rescued by transient transfection with wild-type subunit. Transfection point eliminated PKC phosphorylation Ser880 carboxy-terminal PDZ ligand...
Interactions between NMDA receptors (NMDARs) and the PDZ [postsynaptic density-95 (PSD-95)/Discs large/zona occludens-1] domains of PSD-95/SAP90 (synapse-associated protein with a molecular weight 90 kDa) family proteins play important roles in synaptic targeting signaling NMDARs. However, little is known about mechanisms that regulate these domain-mediated interactions. Here we show casein kinase II (CK2) phosphorylates serine residue (Ser1480) within C-terminal ligand (IESDV) NR2B subunit...
The M channels, important regulators of neuronal excitability, are voltage-gated potassium channels composed KCNQ2-5 subunits. Mutations in KCNQ2 and KCNQ3 cause benign familial neonatal convulsions (BFNC), dominantly inherited epilepsy myokymia. Crucial for their functions controlling the must be placed at specific regions membrane. However, precise distribution surface KCNQ is not known. Here, we show that KCNQ2/KCNQ3 preferentially localized to axons both axonal initial segment more...
Myokines and exosomes, originating from skeletal muscle, are shown to play a significant role in maintaining brain homeostasis. While exercise has been reported promote muscle secretion, little is known about the effects of neuronal innervation activity on yield molecular composition biologically active molecules muscle. As neuromuscular diseases disabilities associated with denervation impact metabolism, we hypothesize that firing may pivotal regulating secretion activities muscles. We...
Excitatory synapses in the brain undergo activity-dependent changes strength of synaptic transmission. Such plasticity as exemplified by long-term potentiation (LTP) is considered a cellular correlate learning and memory. The presence G protein-activated inwardly rectifying K + (GIRK) channels near excitatory on dendritic spines suggests their possible involvement plasticity. However, whether regulation GIRK affects unknown. In companion article we have reported channel density cultured...
G protein-activated inwardly rectifying K(+) (GIRK) channels regulate neuronal excitability by mediating inhibitory effects of protein-coupled receptors for neurotransmitters and neuromodulators. Notwithstanding many studies reporting modulation GIRK channel function, whether activity regulates trafficking remains an open question. Here we report that NMDA receptor activation in cultured dissociated hippocampal neurons elevates surface expression the subunits GIRK1 GIRK2 soma, dendrites,...
Abstract We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nm in diameter, that were stable for over month. made sQDs four emission wavelengths, from 527 655 and with different functional groups. AMPA receptors on live neurons labeled postsynaptic density proteins visualized super‐resolution microscopy. Their diffusion behavior indicates access the synaptic clefts significantly more often than commercial QDs.
Abstract K v 7 channels are enriched at the axonal plasma membrane where their voltage-dependent potassium currents suppress neuronal excitability. Mutations in 7.2 and 7.3 subunits cause epileptic encephalopathy (EE), yet underlying pathogenetic mechanism is unclear. Here, we used novel statistical algorithms structural modeling to identify EE mutation hotspots key functional domains of including voltage sensing S4, pore loop S6 domain, intracellular calmodulin-binding helix B B-C linker....
The neural precursor cell expressed developmentally down-regulated gene 4-2, Nedd4-2, is an epilepsy-associated with at least three missense mutations identified in epileptic patients. Nedd4-2 encodes a ubiquitin E3 ligase that has high affinity toward binding and ubiquitinating membrane proteins. It currently unknown how mediates neuronal circuit activity its dysfunction leads to seizures or epilepsies. In this study, we provide evidence show seizure susceptibility through ubiquitination of...
Synaptic scaling allows neurons to homeostatically readjust synaptic strength upon chronic neural activity perturbations. Although altered has been implicated underlie imbalanced brain excitability in neurological disorders such as autism spectrum and epilepsy, the molecular dysregulation restoration of those diseases have not demonstrated. Here, we showed that homeostatic downscaling is absent hippocampal Fmr1 KO mice, mouse model most common inherited autism, fragile X syndrome (FXS). We...
Homeostatic intrinsic plasticity encompasses the mechanisms by which neurons stabilize their excitability in response to prolonged and destabilizing changes global activity. However, milieu of molecular players responsible for these regulatory is largely unknown.Using whole-cell patch clamp recording unbiased gene expression profiling rat dissociated hippocampal cultured at high density, we demonstrate here that chronic activity blockade induced sodium channel blocker tetrodotoxin leads a...
Primary neuronal cultures have been widely used to study morphology, neurophysiology, neurodegenerative processes, and molecular mechanism of synaptic plasticity underlying learning memory. However, the unique behavioral properties neurons make them challenging study, with phenotypic differences expressed as subtle changes in arborization rather than easy-to-assay features such cell count. The need analyze growth, intracellular transport has motivated development increasingly sophisticated...