Avian reovirus S1133 encodes 10 primary translation products, 8 of which are structural components the viral particle and 2 nonstructural proteins. The identity gene that codes for each these polypeptides was determined by in vitro denatured individual genome segments.

There are a number of discrepancies in the literature regarding protein composition avian reoviruses. The present study demonstrates that reovirus S1133 contains at least 10 proteins (lambdaA, lambdaB, lambdaC, muA, muB, muBC, muBN, sigmaA, sigmaB, and sigmaC). Polypeptides sigmaC components outer capsid layer virus, while lambdaA, sigmaA core polypeptides. Protein lambdaC is component both layers, extending from inner to capsid. minor outer-capsid polypeptide shown be cell attachment...

Infection of chicken embryo fibroblasts by avian reovirus induces an increase in the permeability host plasma membrane at late, but not early, infection times. The absence changes early times, as well dependence late modification on both viral protein synthesis and active exocytic route, suggest that a virus-encoded is required for to permeabilize cells. Further studies revealed expression nonstructural p10 bacterial cells arrested cell growth enhanced permeability. Membrane leakiness was...

The cytopathic effect evidenced by cells infected with avian reovirus S1133 suggests that this virus may induce apoptosis in primary cultures of chicken embryo fibroblasts. In report we present evidence infection cultured causes activation the intracellular apoptotic program and takes place during an early stage viral life cycle. ability reoviruses to is not restricted a particular strain or specific cell type, since different isolates were able several mammalian lines. Apoptosis was also...

The results of a previous study demonstrated that avian reovirus is highly resistant to the antiviral effects interferon and suggested double-stranded RNA (dsRNA)-binding sigmaA protein might play an important role in resistance. To gather more evidence on interferon-inhibitory activity protein, its gene was cloned into prokaryotic maltose-binding (MBP) fusion vector pMalC recombinant vaccinia virus WRS2. two proteins displayed dsRNA-binding affinity similar synthesized reovirus-infected...

Treatment of primary cultures chicken embryo fibroblasts with a recombinant alpha/beta interferon (rcIFN) induces an antiviral state that causes strong inhibition vaccinia virus and vesicular stomatitis replication but has no effect on avian reovirus S1133 replication. The fact polypeptides are synthesized normally in rcIFN-treated cells prompted us to investigate whether this expresses factors interfere the activation and/or activity IFN-induced, double-stranded RNA (dsRNA)-dependent...

Avian reovirus S1133 specifies at least 10 primary translation products, eight of which are present in the viral particle and two nonstructural proteins. In work presented here, we studied covalent modifications undergone by these products infected cell. The structural polypeptide mu2 was shown to be intracellularly modified both myristoylation proteolysis. site-specific cleavage yielded a large carboxy-terminal fragment myristoylated approximately 5,500-Mr peptide corresponding amino...

ABSTRACT It was previously shown that the second open reading frame of avian reovirus S1 gene encodes a 146-amino-acid nonstructural protein, designated p17, which has no known function and sequence similarity to other proteins. The results presented in this report demonstrate p17 accumulates nucleoplasm infected transfected cells. An examination deduced amino acid revealed presence putative monopartite nuclear localization signal (NLS) between residues 119 128. Mutagenesis analysis both is...

Members of the genus Orthoreovirus replicate in cytoplasmic inclusions termed viral factories. Compelling evidence suggests that nonstructural protein microNS forms matrix factories and recruits specific proteins to these structures. In first part this study, we analyzed properties avian reovirus microNS-derived found they are nonaggresome globular structures not associated with cytoskeleton which do require an intact microtubule network for formation maturation. We next investigated...

ABSTRACT Avian reovirus sigmaA is a double-stranded RNA (dsRNA)-binding protein that has been shown to stabilize viral core particles and protect the virus against antiviral action of interferon. To continue with characterization this protein, we have investigated its intracellular distribution in avian cells. Most accumulates into cytoplasmic factories infected cells, yet significant fraction was detected nucleolus. The also localizes nucleolus transfected suggesting nucleolar targeting not...

Inhibitors of vacuolar proton-ATPase activity (5 microM bafilomycin A1 or 50 nM concanamycin A) prevented infection by reovirus particles but not infectious subviral (ISVPs). Neither compound affected virus attachment internalization. However, both compounds potently blocked cleavage the viral protein mu 1C. Finally, and ISVPs efficiently translocated toxin alpha-sarcin to cytosol during entry. Bafilomycin translocation ISVPs.

Previous work has shown that the avian reovirus cell-attachment sigma C (σC) protein is a multimer. In first part of this study oligomerization state intracellularly synthesized σC was analysed by different approaches, including SDS–PAGE, chemical cross-linking, sedimentation and gel filtration analysis. All these approaches indicated in its native homotrimer. second present we investigated effect factors reagents on oligomer stability, order to elucidate nature forces maintain...

Background Avian reoviruses replicate in viral factories, which are dense cytoplasmic compartments estabilished by protein-protein interactions. The non-structural protein muNS forms the factory scaffold that attracts other components a controlled fashion. To create such three-dimensional network, uses several different self-interacting domains. Methodology/Principal Findings In this study we have devised strategy to identify regions containing domains, based on capacity of muNS-derived...

A simple double-stranded RNA mycovirus was detected in a wild-type Botrytis cinerea 55k strain. The virus located the fungus cytoplasm as free particles of approximately 28 nm diameter. possesses single genome segment 1.8 kilobase pairs (kbp) encapsidated within an isometric protein coat whose main structural component is polypeptide 68 kDa. Cells infected with this showed important degree cellular degeneration.