A5046774800- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- DNA and Nucleic Acid Chemistry
- Animal Virus Infections Studies
- Chemical Synthesis and Analysis
- Vector-Borne Animal Diseases
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Metal complexes synthesis and properties
- RNA Interference and Gene Delivery
- Viral Infections and Vectors
- Enzyme Structure and Function
- Tardigrade Biology and Ecology
- Ferrocene Chemistry and Applications
- Animal Disease Management and Epidemiology
- Dendrimers and Hyperbranched Polymers
- RNA and protein synthesis mechanisms
- Erythrocyte Function and Pathophysiology
- Click Chemistry and Applications
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Research and Splicing
- Photoreceptor and optogenetics research
- 3D Printing in Biomedical Research
- Advanced Polymer Synthesis and Characterization
Universidade de Santiago de Compostela
2015-2025
Center for Research in Molecular Medicine and Chronic Diseases
2014-2025
University of Lübeck
2015
Universität Hamburg
2015
University of Tübingen
2015
Nagahama Institute of Bio-Science and Technology
2008
University of Oxford
1997
Abstract A new method is reported herein for screening the biological activity of functional polymers across a consistent degree polymerization and in situ, that is, under aqueous conditions without purification/isolation candidate polymers. In brief, chemical functionality poly(acryloyl hydrazide) scaffold was activated using readily available aldehydes to obtain amphiphilic The transport resulting can be evaluated situ model membranes living cells need tedious isolation purification steps....
There are a number of discrepancies in the literature regarding protein composition avian reoviruses. The present study demonstrates that reovirus S1133 contains at least 10 proteins (lambdaA, lambdaB, lambdaC, muA, muB, muBC, muBN, sigmaA, sigmaB, and sigmaC). Polypeptides sigmaC components outer capsid layer virus, while lambdaA, sigmaA core polypeptides. Protein lambdaC is component both layers, extending from inner to capsid. minor outer-capsid polypeptide shown be cell attachment...
RNA-dependent ATPase and helicase activities have been identified associated with the purified VP6 protein of bluetongue virus, a member Orbivirus genus double-stranded RNA (dsRNA; Reoviridae family) viruses. In addition, has an ATP binding activity. unwinding duplexes occurred both 3' 5' overhang templates, as well blunt-ended dsRNA, activity not previously in other viral helicases. Although little sequence similarity to helicases was detected, certain similarities motifs commonly...
Cu(II) coordination complexes are emerging as promising anticancer agents due to their ability induce oxidative stress through reactive oxygen species (ROS) generation. In this study, we synthesized and characterized two novel metallopeptide systems, 1/Cu(II) 2/Cu(II), derived from the oligocationic bipyridyl cyclopeptides 1 2, designed enhance transport of into cells increase ROS levels. Spectroscopic electrochemical analyses confirmed formation stable in aqueous media. Inductively coupled...
Background/Objectives: Crimean–Congo hemorrhagic fever virus (CCHFV) is an emerging, widely distributed zoonotic tick-borne pathogen. The causes severe disease in humans, and numerous wild domestic animals act as reservoirs of it. Unfortunately, there are no effective therapies or safe vaccines commercialized nowadays for this particular virus. As CCHF (Crimean–Congo fever) a serious threat to public health, urgent need investigate the development vaccination strategies further. Methods: In...
Gripping compounds! A homodimer is formed by linking two monomers of b-ZIP protein basic regions through a photoresponsive azobenzene unit. By simple irradiation at appropriate wavelenghths, this dimer can be switched between isomers which have moderate (trans) and high (cis) sequence-specific affinity for gripping the major groove DNA (see scheme). BR=basic region, ds=double-stranded.
Packende Verbindungen! Zwei Monomere eines bZIP-Proteins wurden an den basischen Bereichen über eine photoempfindliche Azobenzoleinheit zu einem Homodimer verknüpft. Dieses Dimer kann nach Bestrahlung mit Licht geeigneter Wellenlänge isomerisiert werden, wobei die beiden Isomere einer geringen (trans) oder hohen Affinität (cis) in der großen Furche DNA binden (siehe Bild). BB=basische Bereiche, ds=Doppelstrang.
X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed vivo crystal growth of firefly luciferase Green Fluorescent Protein-tagged reovirus μNS live-cell imaging, showing that...
Although largely overlooked in peptide engineering, coordination chemistry offers a new set of interactions that opens unexplored design opportunities for developing complex molecular structures. In this context, we report artificial ligands fold into chiral helicates the presence labile metal ions such as FeII and CoII . Heterochiral β-turn-promoting sequences encode stereoselective folding define physicochemical properties their corresponding complexes. Circular dichroism NMR spectroscopy...
Infection of chicken embryo fibroblasts by avian reovirus induces an increase in the permeability host plasma membrane at late, but not early, infection times. The absence changes early times, as well dependence late modification on both viral protein synthesis and active exocytic route, suggest that a virus-encoded is required for to permeabilize cells. Further studies revealed expression nonstructural p10 bacterial cells arrested cell growth enhanced permeability. Membrane leakiness was...
The results of a previous study demonstrated that avian reovirus is highly resistant to the antiviral effects interferon and suggested double-stranded RNA (dsRNA)-binding sigmaA protein might play an important role in resistance. To gather more evidence on interferon-inhibitory activity protein, its gene was cloned into prokaryotic maltose-binding (MBP) fusion vector pMalC recombinant vaccinia virus WRS2. two proteins displayed dsRNA-binding affinity similar synthesized reovirus-infected...
N1,N3-Bis(4-amidinophenyl)propane-1,3-diamine (BAPPA, R = H) is a bisaminobenzamidine fluorogenic derivative that displays large increase in its emission fluorescence when bound to the minor groove of specific A/T DNA sites (Kd for an AATT site ∼ 79 ± 6 nM). Moreover, structural characteristics BAPPA allow easy introduction functional groups protrude out surface.
Abstract The generation of dendrimers is a powerful tool in the control size and biodistribution polyion complexes (PIC). Using combinatorial screening six (18–243 terminal groups) five oppositely charged PEGylated copolymers, dendrimer‐to‐PIC hierarchical transfer structural information was revealed with PIC diameters that increased from 80 to 500 nm on decreasing dendrimer generation. This rise size, which also accompanied by micelle‐to‐vesicle transition, interpreted according cone‐...
The use of copper-based artificial nucleases as potential anticancer agents has been hampered by their poor selectivity in the oxidative DNA cleavage process. An alternative strategy to solve this problem is design systems capable selectively damaging noncanonical structures that play crucial roles cell cycle. We designed an oligocationic CuII peptide helicate binds and cleaves three-way junctions (3WJs) induces damage via a ROS-mediated pathway both vitro cellulo, specifically at...
Treatment of primary cultures chicken embryo fibroblasts with a recombinant alpha/beta interferon (rcIFN) induces an antiviral state that causes strong inhibition vaccinia virus and vesicular stomatitis replication but has no effect on avian reovirus S1133 replication. The fact polypeptides are synthesized normally in rcIFN-treated cells prompted us to investigate whether this expresses factors interfere the activation and/or activity IFN-induced, double-stranded RNA (dsRNA)-dependent...
Avian reovirus S1133 specifies at least 10 primary translation products, eight of which are present in the viral particle and two nonstructural proteins. In work presented here, we studied covalent modifications undergone by these products infected cell. The structural polypeptide mu2 was shown to be intracellularly modified both myristoylation proteolysis. site-specific cleavage yielded a large carboxy-terminal fragment myristoylated approximately 5,500-Mr peptide corresponding amino...
ABSTRACT It was previously shown that the second open reading frame of avian reovirus S1 gene encodes a 146-amino-acid nonstructural protein, designated p17, which has no known function and sequence similarity to other proteins. The results presented in this report demonstrate p17 accumulates nucleoplasm infected transfected cells. An examination deduced amino acid revealed presence putative monopartite nuclear localization signal (NLS) between residues 119 128. Mutagenesis analysis both is...
Members of the genus Orthoreovirus replicate in cytoplasmic inclusions termed viral factories. Compelling evidence suggests that nonstructural protein microNS forms matrix factories and recruits specific proteins to these structures. In first part this study, we analyzed properties avian reovirus microNS-derived found they are nonaggresome globular structures not associated with cytoskeleton which do require an intact microtubule network for formation maturation. We next investigated...