A5025157687- Urinary Bladder and Prostate Research
- Bladder and Urothelial Cancer Treatments
- Urological Disorders and Treatments
- S100 Proteins and Annexins
- Urinary Tract Infections Management
- Pelvic floor disorders treatments
- Lipid Membrane Structure and Behavior
- Vector-Borne Animal Diseases
- Cellular transport and secretion
- Tissue Engineering and Regenerative Medicine
- Machine Learning and Data Classification
- Neonatal Respiratory Health Research
- Prostate Cancer Diagnosis and Treatment
- Ion channel regulation and function
- Viral Infectious Diseases and Gene Expression in Insects
- Erythrocyte Function and Pathophysiology
- Medical Image Segmentation Techniques
- MicroRNA in disease regulation
- Sphingolipid Metabolism and Signaling
- Extracellular vesicles in disease
- Imbalanced Data Classification Techniques
- Anomaly Detection Techniques and Applications
- Mitochondrial Function and Pathology
- Caveolin-1 and cellular processes
- Viral Infections and Vectors
University of Bern
2012-2024
University Hospital of Bern
2009-2024
Nomura Research Institute
2017
Tufts Medical Center
2009
Institute of Virology of the Slovak Academy of Sciences
2000
University of Oxford
1994-1997
Circulating miRNAs are detected in extracellular space and body fluids such as urine. RNAs can be packaged secreted urinary vesicles (uEVs) thus protected from degradation. Urinary exosome preparations might contain specific miRNAs, relevant biomarkers renal bladder diseases. Major difficulties application of uEVs into the clinical environment high variability low reproducibility uEV isolation methods. Here we used five different methods to isolate compared size distribution, morphology,...
<b>Background:</b> Many patients taking statins often complain of muscle pain and weakness. The extent to which reflects injury is unknown. <b>Methods:</b> We obtained biopsy samples from the vastus lateralis 83 patients. Of 44 with clinically diagnosed statin-associated myopathy, 29 were currently a statin, 15 had discontinued statin therapy before (minimal duration discontinuation 3 weeks). also included 19 who no 20 never taken myopathy. classified muscles as injured if 2% or more fibres...
Abstract Muscle pain and weakness are frequent complaints in patients receiving 3‐hydroxymethylglutaryl coenzymeA (HMG CoA) reductase inhibitors (statins). Many with myalgia have creatine kinase levels that either normal or only marginally elevated, no obvious structural defects been reported only. To investigate further the mechanism mediates statin‐induced skeletal muscle damage, biopsies from statin‐treated non‐statin‐treated were examined using both electron microscopy biochemical...
Epidermal growth factor receptor (EGFR) mutations enable constitutive active downstream signaling of PI3K/AKT, KRAS/ERK and JAK/STAT pathways, promote tumor progression by inducing uncontrolled proliferation, evasion apoptosis migration non-small cell lung cancer (NSCLC). In addition, such EGFR increase the susceptibility patients with NSCLC to tyrosine kinase inhibitor (TKI) therapy, but treated will invariably relapse resistant disease. A global understanding underlying molecular...
The annexins, a family of Ca2+- and lipid-binding proteins, are involved in range intracellular processes. Recent findings have implicated annexin A1 the resealing plasmalemmal injuries. Here, we demonstrate that another member protein family, A6, is also repair lesions induced by bacterial pore-forming toxin, streptolysin O. An injury-induced elevation concentration Ca2+ ([Ca2+]i) triggers repair. highly Ca2+-sensitive A6 responds faster than to [Ca2+]i elevation. Correspondingly, limited...
MicroRNA miR-199a-5p impairs tight junction formation, leading to increased urothelial permeability in bladder pain syndrome. Now, using transcriptome analysis TEU-2 cells, we implicate it the regulation of cell cycle, cytoskeleton remodeling, TGF, and WNT signaling pathways. MiR-199a-5p is highly expressed smooth muscle layer bladder, altered its levels cells (SMCs) validate pathway analysis. Inhibition with antimiR SMC proliferation, reduced size, up-regulated targets, including WNT2....
RNA-dependent ATPase and helicase activities have been identified associated with the purified VP6 protein of bluetongue virus, a member Orbivirus genus double-stranded RNA (dsRNA; Reoviridae family) viruses. In addition, has an ATP binding activity. unwinding duplexes occurred both 3' 5' overhang templates, as well blunt-ended dsRNA, activity not previously in other viral helicases. Although little sequence similarity to helicases was detected, certain similarities motifs commonly...
The spatial targeting of receptors to discrete domains within the plasma membrane allows their preferential coupling specific effectors, which is essential for rapid and accurate discrimination signals. Efficiency signaling further increased by protein lipid segregation membrane. We have previously demonstrated importance raft-mediated in regulation smooth skeletal muscle cell contraction. Since G protein-coupled (GPCRs) are key components contraction-relaxation cycles, it important...
Upon its genesis during apoptosis, ceramide promotes gross reorganization of the plasma membrane structure involving clustering signalling molecules and an amplification vesicle formation, fusion trafficking. The annexins are a family proteins, which in presence Ca(2+), bind to membranes containing negatively charged phospholipids. Here, we show that increases affinity annexin A1-membrane interaction. In physiologically relevant range Ca(2+) concentrations, this leads increase...
Cholesterol regulates plasma membrane (PM) association and functioning of syntaxin-4 soluble N-ethylmaleimide-sensitive fusion protein 23 (SNAP23) in the secretory pathway. However, molecular mechanism cellular cholesterol pools that determine localization assembly these target SNAP receptors (t-SNAREs) are largely unknown. We recently demonstrated high levels annexin A6 (AnxA6) induce accumulation late endosomes, thereby reducing Golgi PM. This leads to an impaired supply needed for...
The annexins are a family of Ca2+- and phospholipid-binding proteins, which interact with membranes upon increase [Ca2+]i or during cytoplasmic acidification. transient nature the membrane binding complicates study their influence on intracellular processes. To address function at plasma (PM), we fused fluorescent protein-tagged A6, A1, A2 H- K-Ras anchors. Stable PM localization membrane-anchored annexin A6 significantly decreased store-operated Ca2+ entry (SOCE), but did not rates...
Defects in urothelial integrity resulting leakage and activation of underlying sensory nerves are potential causative factors bladder pain syndrome, a clinical syndrome pelvic urinary urgency/frequency the absence specific cause. Herein, we identified microRNA miR-199a-5p as an important regulator intercellular junctions. On overexpression cells, it impairs correct tight junction formation leads to increased permeability. directly targets mRNAs encoding LIN7C, ARHGAP12, PALS1, RND1, PVRL1...
Ceramide is a key lipid mediator of cellular processes such as differentiation, proliferation, growth arrest and apoptosis. During apoptosis, ceramide produced within the plasma membrane. Although recent data suggest that generation intracellular increases mitochondrial permeability, source remains unknown. Here, we determine whether stress-mediated plasmalemmal pool might become available to mitochondria apoptotic cells. We have previously established annexin A1—a member family Ca2+...
Bladder outlet obstruction (BOO) induces significant organ remodeling, leading to lower urinary tract symptoms accompanied by urodynamic changes in bladder function. Here, we report mRNA and miRNA transcriptome sequencing of samples from human patients with different urodynamically defined states BOO. Patients' expression profiles correlated findings. Validation RNA results an independent patient cohort identified combinations 3 mRNAs (NRXN3, BMP7, UPK1A) miRNAs (miR-103a-3p, miR-10a-5p,...
Cell membrane compartmentalization, which is believed to involve association of cholesterol- and glycosphingolipid-enriched rafts, represents an important means transmitting information across the plasma membrane. We have previously shown that raft mediated by Ca2+-dependent binding annexin 2 In present study, we demonstrate annexins 1 with smooth muscle cell can be terminated their proteolytic cleavage. This proteolysis thought triggered calpain occurs at non-raft regions It critically...
The pHi (intracellular pH) is an important physiological parameter which altered during hypoxia and ischaemia, pathological conditions accompanied by a dramatic decrease in pHi. Sensors of include ion transport systems control intracellular Ca2+ gradients link changes to functions as diverse proliferation apoptosis. annexins are protein family characterized Ca2+-dependent interactions with cellular membranes. Additionally, vitro evidence points the existence pH-dependent, Ca2+-independent...
Machine learning (ML) has emerged as a vital asset for researchers to analyze and extract valuable information from complex datasets. However, developing an effective robust ML pipeline can present real challenge, demanding considerable time effort, thereby impeding research progress. Existing tools in this landscape require profound understanding of principles programming skills. Furthermore, users are required engage the comprehensive configuration their obtain optimal performance. To...