A5051915794- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Viral Infections and Outbreaks Research
- Animal Disease Management and Epidemiology
- Fire effects on ecosystems
- Animal Virus Infections Studies
- Aquaculture disease management and microbiota
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Infectious Diseases and Tuberculosis
- Mycobacterium research and diagnosis
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Hepatitis B Virus Studies
- Tuberculosis Research and Epidemiology
- Marine Bivalve and Aquaculture Studies
- Plant Disease Resistance and Genetics
- Complement system in diseases
- Parasite Biology and Host Interactions
- Aquatic Invertebrate Ecology and Behavior
- Viral Infectious Diseases and Gene Expression in Insects
- Parvovirus B19 Infection Studies
- Myxozoan Parasites in Aquatic Species
- Invertebrate Immune Response Mechanisms
Centro de Investigación en Sanidad Animal
2011-2024
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2013-2024
Consejo Superior de Investigaciones Científicas
2000-2024
Centro de Astrobiología
2023
Institute of Agrifood Research and Technology
2020
Centro de Biología Molecular Severo Ochoa
2017
Universidad Autónoma de Madrid
2017
Centro Regional de Selección y Reproducción Animal
1995-2014
Hospital General Universitario Gregorio Marañón
2005-2006
Comunidad de Madrid
2006
Abstract Rift Valley Fever virus (RVFV) causes recurrent outbreaks of acute life-threatening human and livestock illness in Africa the Arabian Peninsula. No licensed vaccines are currently available for humans those widely used have major safety concerns. A ‘One Health’ vaccine development approach, which same is co-developed multiple susceptible species, an attractive strategy RVFV. Here, we utilized a replication-deficient chimpanzee adenovirus platform with established profile, ChAdOx1,...
The introduction of three single nucleotide mutations into the genome virulent RVFV ZH548 strain allows for rescue a fully attenuated virus in mice (ZH548-rA2). These are located viral genes encoding RdRp and non-structural protein NSs. This paper shows results obtained after subcutaneous inoculation ZH548-rA2 adult sheep subsequent challenge with parental (ZH548-rC1). Inoculation caused no detectable clinical or pathological effect sheep, whereas rC1 lesions compatible infection...
The increasing interest in Rift Valley fever virus (RVFV) and its potential impact on naive animal populations deserve revisiting experimental reproduction of RVFV infection, particularly those breeds for which no data about their susceptibility to infection have ever been recorded. In this study we show the 9-10 weeks old European sheep (Ripollesa breed) showing a mild, subacute form disease. Four different viral isolates efficiently replicated vivo after subcutaneous inoculation,...
ABSTRACT. Marteilia refringens is one of the most significant pathogens bivalve molluscs. Previous sequencing small subunit ribosomal RNA gene M. isolates derived from infected mussels ( Mytilus edulis and galloprovinciallis ) oyster Ostrea in Europe did not reveal genetic polymorphisms despite indications epizootiological data that distinct types may exist. We investigated existence internal transcribed spacer region genes. The sequences this proved to be clearly dimorphic among five...
Rift Valley Fever (RVF) is a viral zoonosis that historically affects livestock production and human health in sub-Saharan Africa, though epizootics have also occurred the Arabian Peninsula. Whilst an effective live-attenuated vaccine available for livestock, there currently no licensed RVF vaccine. Replication-deficient chimpanzee adenovirus (ChAd) vectors are ideal platform development of vaccine, given low prevalence neutralizing antibodies against them population, their excellent safety...
Background Rift Valley fever virus (RVFV) is a mosquito-borne pathogen causing an important disease in ruminants often transmitted to humans after epizootic outbreaks African and Arabian countries. To help combat the spread of disease, prophylactic measures need be developed and/or improved. Methodology/Principal Findings In this work, we evaluated immunogenicity protective efficacy recombinant plasmid DNA modified vaccinia Ankara (rMVA) vectored vaccines against mice. These encoded either...
Abstract In the present study, we evaluated immunogenicity, safety and protective efficacy of attenuated RVFV-40Fp8 strain in natural hosts (non-pregnant ewes) a highly susceptible host infection model such as pregnant ewes first third pregnancy. Our results confirm immunogenicity 40Fp8 administration non-pregnant ewes, well absence foetal damage even after high-dose vaccination regime ewes. addition, their foetuses were protected against virulent RVFV-56/74 challenge, shown by comparative...
Background Mesenchymal stromal cells (MSCs) are a population of undifferentiated non-hematopoietic fibroblast-like isolated from several tissues with multipotent differentiation capacity in vitro. This study focused on the establishment and characterization mesenchymal cell line derived ovine peripheral blood mononuclear (PBMCs). Methods MSC were used to develop line. The confirm its origin was carried out by different assays. First at all, characterized flow cytometry (FACS) using...
Background/Objectives: Crimean–Congo hemorrhagic fever virus (CCHFV) is an emerging, widely distributed zoonotic tick-borne pathogen. The causes severe disease in humans, and numerous wild domestic animals act as reservoirs of it. Unfortunately, there are no effective therapies or safe vaccines commercialized nowadays for this particular virus. As CCHF (Crimean–Congo fever) a serious threat to public health, urgent need investigate the development vaccination strategies further. Methods: In...
This paper describes the generation of monoclonal antibodies directed to immunogenic nucleoprotein N epitopes Rift Valley fever virus (RVFV), and their application in diagnostics, both for antibody detection competitive ELISA antigen capture a sandwich ELISA. Monoclonal (mAbs) were generated after DNA immunization Balb/c mice characterized by western blot, cell immunostaining assays. At least three different immunorelevant defined mAb competition Interestingly, two mAbs able distinguish...
Abstract Rift Valley fever (RVF) and bluetongue (BT) are two important ruminant diseases transmitted by arthropods. Both viruses have shown geographic spread leading to endemicity of BT virus (BTV) in Africa Europe. In this work, we report a dual vaccine that simultaneously induces protective immune responses against BTV RVFV based on modified vaccinia Ankara (MVA) expressing proteins VP2, NS1, or truncated form NS1 (NS1-Nt), Gn Gc glycoproteins. IFNAR (−/−) mice immunized with doses...
The COVID-19 pandemic has underscored the importance of swift responses and necessity dependable technologies for vaccine development. Our team previously developed a fast cloning system modified vaccinia virus Ankara (MVA) platform. In this study, we reported on construction preclinical testing recombinant MVA obtained using system. We expressing unmodified full-length SARS-CoV-2 spike (S) protein containing D614G amino-acid substitution (MVA-Sdg) version S substitutions designed to...
Extrarespiratory tuberculosis is determined mainly by impaired immunity of the host. The additional role played bacterial factors in determining whether an infection Mycobacterium disseminates to extrarespiratory sites has not been analyzed depth. In present study, we selected patients who were dually infected with 2 M. strains but whom only one sites, whereas other strain remained at respiratory site. We compared infectivity and a newly designed ex vivo competitive macrophage coinfection...
African horse sickness virus (AHSV) belongs to the genus Orbivirus. We have now engineered naked DNAs and recombinant modified vaccinia Ankara (rMVA) expressing VP2 NS1 proteins from AHSV-4. IFNAR(−/−) mice inoculated with DNA/rMVA-VP2,-NS1 AHSV-4 in an heterologous prime-boost vaccination strategy generated significant levels of neutralizing antibodies specific In addition, stimulated T cell responses against virus. The vaccine elicited partial protection homologous infection induced...
The aim of this work was to evaluate the immunogenicity and efficacy DNA MVA vaccines encoding RVFV glycoproteins Gn Gc in an ovine model infection. Adult sheep both sexes were challenged 12 weeks after last immunization clinical, virological, biochemical immunological consequences, analyzed. Strategies based on with homologous or heterologous DNA/MVA prime-boost able induce a rapid vitro neutralizing antibody response as well IFNγ production virus specific re-stimulation. In these animals...
The sequence of non-structural protein NS1 bluetongue virus (BTV), which contains immunodominant CD8+ T cell epitopes, is highly conserved among BTV serotypes, and has therefore become a major tool in the development universal vaccine. In this work, we have engineered multiserotype vaccine candidates based on recombinant chimpanzee adenovirus (ChAdOx1) modified vaccinia Ankara (MVA) vectors expressing BTV-4 or its truncated form NS1-Nt. A single dose ChAdOx1-NS1 ChAdOx1-NS1-Nt induced...