A study of the peculiarities and a comparative analysis technologies used for fabrication elements novel hybrid microfluidic biochips express biomedical have been carried out. The were designed with an incorporated system, which enabled accumulation target compounds in biological fluid to be achieved, thus increasing biochip system's sensitivity even implementing label-free design detection unit. multilevel process manufacturing system given topology fluorometric protein structures is...

The problems of chronic or noncommunicable diseases (NCD) that now kill around 40 million people each year require multiparametric combinatorial diagnostics for the selection effective treatment tactics. This could be implemented using biosensor principle based on peptide aptamers spatial recognition corresponding protein markers in biological fluids. In this paper, a low-cost label-free biomarker detection system fluorometric registration target proteins bound to was investigated. main...

The in silico modelling of peptides complementary to lactoferrin was carried out using the Protein 3D software package and replication natural bonding site between pneumococcal surface protein (PSP) (LF). modeling based on analysis conjugated ion–hydrogen bond systems these proteins (CIHBS). oligopeptide EEVAPQAQAKIAELENQVHRLE proposed via computer synthesized solid phase synthesis technique, purified, analyzed with MS HPLC methods confirm >95% purity. peptide then studied by capillary...

Magnetic parameters of magnetosomes inside the bacteria MSR-1, LBB-42, AMB-1, SP-1, BB-1, and SO-1 strains genus Magnetospirillum fixed by 5% formalin in nutrient medium were estimated measurements nonlinear longitudinal response to a weak ac magnetic field (NLR-M2) at room temperature. For AMB-1 strains, electron resonance (EMR) spectra with special X-band spectrometer for wide-line registration also carried out. To trace evolution state during long-term storage, freshly prepared samples...

Oncotheranostic drugs based on magnetic nanoparticles (MNPs) are a promising method for the treatment and diagnosis of cancer. Control parameters properties MNPs is important both at all stages their synthesis during direct use in clinical practice. This work considers microfluidic approach to assessing diffusion various types. Using image analysis channel, coefficients ferromagnetic liposomes were determined. Also, using systems, behavior these particles suspension-hydrogel interface...

The development of integrated biosensor systems based on the optical detection principle dictates need to ensure passage a pure biological sample into zone. In particular, detect protein structures their natural fluorescence, it is necessary achieve minimum number blood cells in area, since they make parasitic contribution fluorescent signal. To solve this problem, work we used planar traps made dry film photoresist. Various geometries were manufactured and tested using model microobjects....

A hybrid microfluidic biochip was studied for label-free recording of the formation a complex between target proteins and peptide aptamers that are selective them have spatial complementarity properties. Troponin T lactoferrin, which markers acute cardiovascular conditions, were used as proteins. The detection principle registration natural fluorescence protein structures with its re-emission to longer wavelength region in order simplify using standard CMOS matrix. structure chip ensured...

The design of oligopeptides, spatially complementary to blood protein-markers, such as myeloperoxidase (MPO) - a neutrophil enzyme which is risk factor myocardial infarction, stroke and other acute diseases, an important direction in development express-diagnostic tools, particularly biochips. To develop tools the visualization analysis protein structures needed, was carried out using Protein 3D software developed earlier at ETU. In this program conjugated ionic- hydrogen bonds systems...

The development of technologies for manufacturing elements novel hybrid microfluidic biosensors intended portable express biomedical analysis systems have been carried out. system is an important functional element a low-power label-free optical detecting protein structures based on molecular recognition. process given topology fluorometric detection presented. technological included formation the relief system, which SU-8 and dry film photoresist were studied deposition solid-state...

The principles of the development MEMS/NEMS biosensor systems for rapid clinical and preclinical analysis diseases with sensor elements based on molecular recognition target protein markers by specifically designed peptide oligomers are considered. aim this work is to search sequences α-oligopeptide chain molecules, which, due specificity side groups their amino acids, form a 3D structure that complementary globular proteins. At stage, «Protein 3D» software was used oligopeptides, which...

Impedimetric biosensors are used for detecting a wide range of analytes. The detection principle is perspective the development new types analytical devices biomolecular diagnosis diseases. Of particular interest with very high sensitivities, capable trace amounts biomarkers or drugs in biological fluids. possess potential increased sensitivity, since their electrodes can be modified nanostructured materials, zinc oxide. In this work, miniature biosensor an array oxide nanorods synthesized...

The antibiotic resistance (ABR) problem is becoming increasingly disturbing and it important to implement express methods of ABR testing allow operative therapy decisions. application laser light scattering (LLS) in microbiological analysis for microorganisms has been considered. ways miniaturization creating the bases their integration into laboratory-on-a-chip (MLOC) clinical diagnostics have analysed. advantage context realization problems are investigated. A system parallel measuring...

A miniature impedimetric sensor element (I SE), integratable into a laboratory-on-a-chip for testing the antibiotic resistance of microorganisms is described. The topology, design and manufacturing technology ISE based on interdigital electrodes are With help sample chips, measurements were made evaluation (ABR) microorganisms, using E. coli M-17 as model, under influence number antibiotics. possibility developed ABR estimation at exposure time 1 to 2 hours shown experimentally....

Biosensor system based on 3D peptide-protein molecular recognition mechanism for rapid medical diagnostics of diseases by determination their protein biomarkers in biological fluids has been considered. The diagnostic was implemented as micro-arrays with topologically structured sites bonded peptide aptamers (PMA). perspective structures were analyzed using the Conjugated Ion-Hydrogen Bonds System (CIHBS) developed earlier. A number spartial complemetarity to myoglobin and troponin designed,...

The layout of hybrid-integrated laboratory on-a-chip and features its main functional elements are presented. architecture this new generation miniature device for express identification antibiotic resistance testing microorganisms, physical principles technologies creating components their integration into a single system discussed. microbiological sample is processed analyzed on the basis miniaturization "digitalization" via generating individual bacteria microcolonies about 1000 CFU in...

The design and technology of sensor/actuator elements (SAE) based on SAW principle, integrated into hybrid analytical microdevices (laboratories-on-a-chip) have been considered. sample element was implemented the lithium niobate crystal 128 ° YX LiNbO3 by using metal deposition standard lithography approaches. working frequency range 10 - 17 MHz. SAE with microfluidic system for transport biological fluids. topology frequency/amplitude regimes were optimized in order to increase precision...

The development and research of antitumor drugs requires the creation new solutions for personalized medicine, in particular, integrated devices testing effectiveness based on microfluidic systems. Such systems allow rapid assessment small volumes samples both cells supracellular structures, including those taken from real patients. In this work, such a system is implemented basis polymer materials using laser ablation thermal bonding technologies with electrodes. With their help, an...

This study addresses issues in developing spatially controlled magnetic fields for particle guidance, synthesizing biocompatible and chemically stable MNPs enhancing their specificity to pathological cells through chemical modifications, personalized adjustments, highlighting the potential of tumor-on-a-chip systems, which can simulate tissue environments assess drug efficacy dosage a setting. The research focused on two MNP types, uncoated magnetite nanoparticles (mMNPs) carboxymethyl...