A5007900795- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- Genomics and Chromatin Dynamics
- HIV Research and Treatment
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Cancer-related gene regulation
- Parvovirus B19 Infection Studies
- Cell death mechanisms and regulation
- DNA Repair Mechanisms
- Hepatitis Viruses Studies and Epidemiology
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Diabetes and associated disorders
- Eosinophilic Disorders and Syndromes
- Immunotherapy and Immune Responses
Mohammed Bin Rashid University of Medicine and Health Sciences
2024
University of Geneva
2017-2022
McMaster University Medical Centre
2016-2017
Université Claude Bernard Lyon 1
2008-2012
Inserm
2008-2012
Centre de Recherche en Cancérologie de Lyon
2012
Centre National de la Recherche Scientifique
2012
The D614G mutation in the Spike protein of SARS-CoV-2 has effectively replaced early pandemic-causing variant. Using pseudotyped lentivectors, we confirmed that aspartate replacement by glycine position 614 is markedly more infectious. Molecular modelling suggests G614 facilitates transition towards an open state protein. To explain epidemiological success D614G, analysed evolution 27,086 high-quality genome sequences from GISAID. We observed striking coevolution with P323L viral polymerase....
Enterovirus 71 (EV71) causes hand, foot and mouth disease, a mild self-limited illness that is sometimes associated with severe neurological complications. EV71 neurotropic determinants remain ill-defined to date. We previously identified mutation in the VP1 capsid protein (L97R) was acquired over course of disseminated infection an immunocompromised host. The absent respiratory tract but present gut (as mixed population) blood cerebrospinal fluid dominant species). In this study, we...
Infection with hepatitis B virus (HBV) led to 887,000 human deaths in 2015. HBV has been coevolving mammals for millions of years. Recently, the Smc5/6 complex, which essential housekeeping functions, was identified as a restriction factor antagonized by regulatory HBx protein. Here we address whether antiviral activity is an important evolutionarily conserved function. We found that all six subunits have primates, only Smc6 showing signatures “evolutionary arms race.” Using evolution-guided...
Abstract The D614G mutation of the Spike protein is thought to be relevant for SARS-CoV-2 infection. Here we report biological and epidemiological aspects this mutation. Using pseudotyped lentivectors, were able confirm that G614 variant markedly more infectious than ancestral D614 variant. We demonstrate by molecular modelling replacement aspartate glycine in position 614 facilitates transition towards an open state protein. To understand whether increased infectivity explains its success,...
Cationic cell-penetrating peptides (CPPs) and their lipid domain-conjugates (CatLip) are agents for the delivery of (uncharged) biologically active molecules into cell. Using infection transfection assays we surprisingly discovered that CatLip were able to inhibit replication Duck Hepatitis B Virus (DHBV), a reference model human HBV. Amongst twelve identified Deca-(Arg)8 having particularly potent antiviral activity, leading drastic inhibition viral particle secretion without detectable...
Not all antibodies against SARS-CoV-2 inhibit viral entry, and hence, infection. Neutralizing are more likely to reflect real immunity; however, certain tests investigate protein/protein interaction rather than the fusion event. Viral pseudoviral entry assays detect functionally active but limited by biosafety standardization issues. We have developed a Spike/ACE2-dependent assay, based on split luciferase. Hela cells stably transduced with Spike large fragment of luciferase were co-cultured...
ABSTRACT Not all antibodies against SARS-CoV-2 inhibit viral entry and hence infection. Neutralizing are more likely to reflect real immunity, however certain of these tests investigate protein/protein interaction rather than the fusion event. Viral pseudoviral assays detect functionally active antibodies, they cumbersome burdened by biosafety standardization issues. We have developed a Spike/ACE2-dependant cell-to-cell assay, based on split luciferase. Hela cells stably transduced with...
Abstract Infection with Hepatitis B virus (HBV) is a major cause of liver disease and cancer in humans. HBVs (family Hepadnaviridae ) have been associated mammals for millions years. Recently, the Smc5/6 complex, known its essential housekeeping functions genome maintenance, was identified as an antiviral restriction factor human HBV. The has however developed counteraction mechanism by degrading complex via regulatory HBx protein. Whether activity against hepadnaviruses important...
Background: The SARS-CoV-2 pandemic was particularly devastating for elderly people, and the underlying mechanisms of disease are still poorly understood. In this study, we investigated fusion inhibitory antibodies (fiAbs) in younger COVID-19 patients analyzed predictive factors their occurrence. Methods: Data samples were collected two cohorts hospitalized patients. A assay spike-expressing cells with ACE2-expressing used to quantify fiAbs serum Results: total 108 (52 (mean age 85 ± 7...