A5061419619- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- vaccines and immunoinformatics approaches
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Immune Response and Inflammation
- Food Allergy and Anaphylaxis Research
- Clostridium difficile and Clostridium perfringens research
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Salmonella and Campylobacter epidemiology
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Viral Infections and Vectors
- Virology and Viral Diseases
- Reproductive System and Pregnancy
- Advanced Control Systems Optimization
- HIV/AIDS Research and Interventions
- Gut microbiota and health
National Institute of Allergy and Infectious Diseases
2023-2024
National Institutes of Health
2023-2024
Naval Medical Research Command
2013-2024
Henry M. Jackson Foundation
2017-2024
Uniformed Services University of the Health Sciences
2018-2023
University Health System
2023
Naval Health Research Center
2023
Jackson Foundation
2020-2021
Norsk Hydro (Norway)
2016
Johns Hopkins Medicine
2006-2015
Background. Enterotoxigenic E. coli (ETEC) is a principal cause of diarrhea in travelers, deployed military personnel, and children living low to middle-income countries. ETEC expresses variety virulence factors including colonization (CF) that facilitate adherence the intestinal mucosa. We assessed protective efficacy tip-localized subunit CF antigen I (CFA/I), CfaE, delivered intradermally with mutant heat-labile enterotoxin, LTR192G, controlled human infection model (CHIM). Methods. Three...
The US National Institute of Allergy and Infectious Diseases (NIAID), part the Health (NIH), convened a virtual workshop on August 8-9th, 2023 to explore potential synergies between HIV vaccine approaches that are designed induce cellular or humoral immune responses. goal this was review data leading candidates discuss best strategies for combining these optimize immunity against HIV. Here, we summarize findings reviewed at knowledge gaps priorities future studies will help accelerate...
Enterotoxigenic
Attenuated yellow fever (YF) virus 17D/17DD vaccines are the only available protection from YF infection, which remains a significant source of morbidity and mortality in tropical areas world. The attenuated vaccine, is used worldwide, generates both long-lasting neutralizing antibodies strong T-cell responses. However, on rare occasions, this vaccine has toxic side effects that can be fatal. This study presents design two non-viral DNA-based antigen formulations characterization their...
Enterotoxigenic Escherichia coli (ETEC) is a common cause of infectious diarrhoea and leading morbidity mortality in children living resource-limited settings. It also the travellers' among civilian military travellers. Its dual importance global public health travel medicine highlights need for an effective vaccine. ETEC express colonization factors (CFs) that mediate adherence to small intestine. An epidemiologically prevalent CF surface antigen 6 (CS6). We assessed safety immunogenicity...
Introdução: Com o advento das medidas sanitárias de distanciamento social as Instituições Ensino Superior foram orientadas a suspender suas atividades presenciais provocando uma rápida adoção Ambientes Virtuais Aprendizagem para continuidade do trabalho pedagógico dos diversos cursos. Objetivo: conhecer dimensões principais problemas relacionados ao ensino remoto enfrentados por estudantes curso medicina e enfermagem durante período pandêmico. Método: Trata-se um estudo cunho qualitativo...
Abstract Ag processing is a critical step in defining the repertoire of epitope-specific immune responses. In present study, HIV-1 p55Gag was synthesized as DNA plasmid with either lysosomal-associated membrane protein-1 (LAMP/gag) or human dendritic cell-LAMP (DC-LAMP/gag) and used to immunize mice. Analysis cellular trafficking these two chimeras demonstrated that both molecules colocalized MHC class II but differed their overall endosomal/lysosomal compartments. Following immunization,...
Previous studies of HIV-1 p55Gag immunization mice have demonstrated the usefulness targeting antigens to cellular compartment containing major histocompatibility complex type II (MHC II) molecules by use a DNA antigen formulation encoding Gag as chimera with mouse lysosome-associated membrane protein (mLAMP/gag). In present study, we analyzed magnitude and breadth Gag-specific T-lymphocyte antibody responses elicited in Rhesus macaques after human LAMP/gag (hLAMP/gag) chimera. ELISPOT...
Epitopes from all available full-length sequences of yellow fever virus (YFV) and dengue (DENV) restricted by Human Leukocyte Antigen class I (HLA-I) alleles covering 12 HLA-I supertypes were predicted using the NetCTL algorithm. A subset 179 YFV 158 DENV epitopes selected EpiSelect algorithm to allow for optimal coverage viral strains. The synthesized approximately 75% found bind restricting HLA molecule with an affinity, KD, stronger than 500 nM. immunogenicity 25 HLA-A*02:01, 28...
Enterotoxigenic Escherichia coli (ETEC) is a major cause of infectious diarrhea in children, travelers, and deployed military personnel. As such, development vaccine would be advantageous for public health. One strategy to use subunits colonization factors combined with antigen/adjuvant toxoids as an ETEC vaccine. Here, we investigated the intradermal (i.d.) or sublingual (s.l.) delivery CFA/I fimbrial antigens, including CfaEB CfaE-heat-labile toxin B subunit (LTB) chimera admixed double...
<i>Background:</i> Maternal exposure to environmental ubiquitous allergens could exert an influence on the newborn’s immune repertoire and later development of allergy. The aim this study was investigate effects maternal immunization with <i>Dermatophagoides pteronyssinus</i> (Dp) hypersensitivity response IgG subclass production in offspring using a murine model. <i>Methods:</i> A/Sn mice were immunized Dp before mating normal males. Diaplacental serum...
Robust and well-established immunological assays firm immune correlates of protection that can predict disease outcome and/or vaccine efficacy are essential to adequately assess human responses infection vaccination. The availability reagents calibrated controls is also critically important standardize generate comparable results among different laboratories. workshop "Human Immune Responses against Shigella ETEC: Current Advances the Path Forward" held during VASE meeting provided an...
Many pathogens establish infection at mucosal surfaces such as the enteric pathogen Enterotoxigenic E. coli (ETEC). Thus, there is a pressing need for effective vaccination strategies that promote protective immunity surfaces. Toll-like receptor (TLR) ligands have been extensively developed vaccine adjuvants to systemic immunity, whereas attenuated bacterial toxins including cholera toxin and heat-labile (LT) initially immunity. Here we evaluate ability of TLR4 agonist second-generation...
Enterotoxigenic Escherichia coli (ETEC) strains cause infectious diarrhea and colonize host intestine epithelia via surface-expressed colonization factors. Colonization factor antigen I (CFA/I), a prevalent ETEC factor, is vaccine target since antibodies directed to this fimbria can block adherence prevent diarrhea.Two recombinant antigens derived from CFA/I were investigated with adjuvant system that displays soluble on the surface of immunogenic liposomes. The first antigen, CfaEB,...
CS6, a prevalent surface antigen expressed in nearly 20% of clinical enterotoxigenic Escherichia coli (ETEC) isolates, is comprised two major subunit proteins, CssA and CssB. Using donor strand complementation, we constructed panel recombinant proteins 1 to 3 subunits that contained combinations and/or CssB strand, C-terminal extension 16 amino acids was derived from the N terminus either or While entire could be obtained as soluble, folded it observed possessing heterologous CS6 different...
Enterotoxigenic Escherichia coli (ETEC), Campylobacter jejuni (CJ), and Shigella sp. are major causes of bacterial diarrhea worldwide, but there no licensed vaccines against any these pathogens. Most current approaches to ETEC based on recombinant proteins that involved in virulence, particularly adhesins. In contrast, CJ have included conjugate which lipopolysaccharides (LPS) or capsule polysaccharides chemically conjugated proteins. We explored the feasibility developing a multi-pathogen...
Enterotoxigenic E. coli (ETEC) is a leading cause of diarrhea in travelers as well for children living low- to middle-income countries. ETEC adhere intestinal epithelium via colonization factors (CFs). CFA/I, common CF, composed polymeric stalk and tip-localized minor adhesive subunit, CfaE. Vaccine delivery by the transcutaneous immunization dscCfaE was safe but poorly immunogenic phase 1 trial when administered volunteers with LTR(192G) mLT. To potentially enhance immunogenicity CfaE while...