A5068112355- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Liver physiology and pathology
- Estrogen and related hormone effects
- Connexins and lens biology
- Acne and Rosacea Treatments and Effects
- Extracellular vesicles in disease
- HIV Research and Treatment
- Peptidase Inhibition and Analysis
- Blood groups and transfusion
- Immunodeficiency and Autoimmune Disorders
- Nuclear Receptors and Signaling
- Click Chemistry and Applications
- Protease and Inhibitor Mechanisms
- Biochemical and Structural Characterization
- Cell Adhesion Molecules Research
- Peroxisome Proliferator-Activated Receptors
- Hormonal Regulation and Hypertension
- Calpain Protease Function and Regulation
University of Miami
2019-2022
University of Montana
2019
Washington State University
2013-2015
University of Colorado Colorado Springs
2011
Developing simple and effective approaches to detect tumor markers will be critical for early diagnosis or prognostic evaluation of prostate cancer treatment. Prostate‑specific membrane antigen (PSMA) has been validated as an important marker progression including angiogenesis metastasis. As a type II protein, PSMA can constitutively internalized from the cell surface into endosomes. Early endosomes fuse with multivesicular bodies (MVB) form secrete exosomes (40-100 nm) extracellular...
Long-term delivery of anti-HIV monoclonal antibodies using adeno-associated virus (AAV) holds promise for the prevention and treatment HIV infection. We previously reported that after receiving a single administration AAV vector coding anti-SIV antibody 5L7, monkey 84-05 achieved high levels AAV-delivered 5L7 IgG1 in vivo which conferred sterile protection against six successive, escalating dose, intravenous challenges with highly infectious, pathogenic SIVmac239, including final challenge...
The repressive states of nuclear receptors (i.e., apo or bound to antagonists inverse agonists) are poorly defined, despite the fact that a major drug target. Most ligand structures receptors, including peroxisome proliferator-activated receptor γ (PPARγ), similar structure. Here we use NMR, accelerated molecular dynamics and hydrogen-deuterium exchange mass spectrometry define PPARγ structural ensemble. We find helix 3 charge clamp positioning varies widely in is stabilized by efficacious...
Understanding the molecular mechanism of prostate cancer progression from androgen dependence to independence may lead developing more effective treatments against cancer. Herein, our previous in vitro model was employed assess effects continuous androgen-deprivation on metastatic phenotype androgen-dependent cells (LNCaP). The results indicated that long-term deprivation resulted overexpression calpain 2 and increased expression filamin A (FlnA), but not for 1. enhanced activity confirmed...
Elucidating the role of androgen deprivation in transition from androgen-dependence to independence may enable development more specific therapeutic strategies against prostate cancer. Our previous vitro model was employed further assess effects continuous androgen‑deprivation on cancer cells (LNCaP) with respect both receptor (AR) and c-Met expression. The results indicated that long-term resulted a signaling pathway switch AR androgen-sensitive cells, which confirmed by immunofluorescence...
This undergraduate organic laboratory experiment consists of three different reactions occurring in the same flask: a cycloaddition reaction, preceded by decarboxylation and nucleophilic substitution reactions. The occur using identical Cu(I) catalyst conditions. Orange, lemon, other citrus fruit peels juices presence CuSO4 lead to Cu(I)-catalyzed 3-phenyl-2-propynoic acid give ethynylbenzene. These click chemistry conditions azide–alkyne (CuAAC) 2-azido-1-phenylethanone with ethynylbenzene...
BACKGROUND Prostate-specific membrane antigen (PSMA) remains an important target for diagnostic and therapeutic application human prostate cancer. Model cell lines have been recently developed to study canine cancer but their PSMA expression enzymatic activity not elucidated. The present was focused on determining in these model the use of fluorescent small-molecule enzyme inhibitors detect by flow cytometry. METHODS Western blot RT-PCR were used determine transcriptional translational Leo...
Membrane-type I matrix metalloproteinases (MT1-MMP) is an enzyme critical to the remodeling and homeostasis of extracellular matrix, when over expressed it contributes metastasis cancer cell progression. Because its role implication as a biomarker that upregulated in various cancers, MT1-MMP has become attractive target for drug discovery. A small pilot library peptidomimetics containing phosphoramidate core zinc-binding group was synthesized tested inhibitory potency against MT1-MMP. From...
Retinoid-based therapies are commonly used in the treatment of disorders keratinization and other skin but can result non-specific effects adverse reactions. Use retinoic acid metabolism blocking agents (RAMBAs) such as DX308 may address these shortcomings.Characterize therapeutic potential recently discovered, CYP26-selective RAMBA, DX308.Preliminary vitro assessment off-target activity, metabolic toxicologic profiling. Studies to assess safety efficacy topical correcting abnormal...