A5008134125- Chemical Synthesis and Analysis
- Antimicrobial Peptides and Activities
- Click Chemistry and Applications
- X-ray Diffraction in Crystallography
- Antibiotic Resistance in Bacteria
- Crystallization and Solubility Studies
- Peptidase Inhibition and Analysis
- Antimicrobial Resistance in Staphylococcus
- Proteoglycans and glycosaminoglycans research
- Synthesis and Catalytic Reactions
- Cell Adhesion Molecules Research
- Burn Injury Management and Outcomes
- Wound Healing and Treatments
- Cellular Mechanics and Interactions
- Cancer therapeutics and mechanisms
- Orthopedic Infections and Treatments
- Synthetic Organic Chemistry Methods
- Natural product bioactivities and synthesis
- Biochemical and Molecular Research
- Advanced biosensing and bioanalysis techniques
- 14-3-3 protein interactions
- Carbohydrate Chemistry and Synthesis
- Advanced Proteomics Techniques and Applications
- Pneumonia and Respiratory Infections
- Urinary Tract Infections Management
The University of Melbourne
2017-2025
RTI International
2025
Monash University
2023-2024
Australian Regenerative Medicine Institute
2023-2024
Discovery Institute
2024
RMIT University
2022
Indian Institute of Science Education and Research Pune
2016
Indian Institute of Science Bangalore
2014
Despite advances in solid phase peptide synthesis and ligation, challenges remain the assembly of polypeptides through coupling fragments. Herein we describe a new method for peptide...
Outer membrane vesicles (OMVs) secreted by Gram-negative bacteria serve as transporters for the delivery of cargo such virulence and antibiotic resistance factors. OMVs play a key role in defense against membrane-targeting antibiotics polymyxin B. Herein, we conducted comparative proteomics from paired Klebsiella pneumoniae ATCC 700721 polymyxin-susceptible (polymyxin B MIC = 0.5 mg/L) an extremely resistant ≥128 mg/L), following exposure to 2 mg/L Comparative profiling OMV subproteome each...
Abstract The impact of geometrically constrained cis α,β‐unsaturated γ‐amino acids on the folding α,γ‐hybrid peptides was investigated. Structure analysis in single crystals and solution revealed that carbon–carbon double bonds can be accommodated into 12‐helix without deviation from overall helical conformation. structures are stabilized by 4→1 hydrogen bonding a similar manner to 12‐helices β‐peptides 3 10 helices α‐peptides. These results show functional backbone peptides.
Peptide macrocyclization is often a slow process, plagued by epimerization and cyclodimerization. Herein, we describe new method for peptide employing the AgI -promoted transformation of thioamides. The has dual function: chemoselectively activating thioamide tethering N-terminal to C-terminal carboxylate. Extrusion Ag2 S generates an isoimide intermediate, which undergoes acyl transfer generate native cyclic peptide, resulting in rapid, traceless macrocylization process. Cyclic peptides are...
Abstract The Ag I ‐promoted reaction of thiolactams with N‐ Boc amino acids yields an N ‐(α‐aminoacyl) lactam that can rearrange through acyl transfer process. Boc‐deprotection results in convergence to the ring‐expanded adduct, thereby facilitating overall insertion acid into thioamide bond generate medium‐sized heterocycles. Application site‐specific cyclic peptides is demonstrated.
Peptide-Peptide Nucleic Acid (PNA) conjugates targeting essential bacterial genes have shown significant potential in developing novel antisense antimicrobials. The majority of efforts this area are focused on identifying different PNA targets and the selection peptides to deliver peptide-PNA Gram-negative bacteria. Notably, a linkage strategy form conjugate plays an important role effective delivery PNAs. Recently, unique Cysteine- 2-Cyanoisonicotinamide (Cys-CINA) click chemistry has been...
Nitrile-aminothiol conjugation (NATC) stands out as a promising biocompatible ligation technique due to its high chemo-selectivity. Herein we investigated the reactivity and substrate scope of NAT chemistry, thus developing novel pH dependent orthogonal NATC valuable tool for chemical biology. The study reaction kinetics elucidated that combination heteroaromatic nitrile aminothiol groups led formation an optimal bioorthogonal pairing, which is dependent. This pairing system was effectively...
A significant increase in life-threatening infections caused by Gram-negative "superbugs" is a serious threat to global health. With dearth of new antibiotics the developmental pipeline, with novel mechanisms action are urgently required prevent return preantibiotic era. key strategy develop anti-infective treatments discover natural scaffolds distinct action. Laterocidine unique cyclic lipodepsipeptide activity against multiple problematic multidrug-resistant pathogens, including
Dityrosine cross-linking of Aβ peptides and α-synuclein is increasingly becoming recognized as a biomarker neuropathological diseases. However, there remains need for the development analytical methods that enable specific selective identification dityrosine cross-linked proteins in complex biological samples. Here, we report gas-phase fragmentation protonated under ultraviolet photodissociation (UVPD) tandem mass spectrometry (MS/MS) conditions results cleavage across Cα Cβ atoms residue....
Abstract Bicyclic peptides are conformationally rigid molecules that can bind with high affinity and specificity to their protein target, yet significantly more protease stable than linear or monocyclic counterparts. This emerging class of has great potential for the development novel peptide therapeutics molecular probes. In this review, we highlight structural complexity, synthesis/biosynthesis, biological applications bridged bicyclic peptides.
We have engineered biomaterials that display nanoclusters of ligands bind both integrin and syndecan-4 cell receptors. These surfaces regulate behaviors under static conditions including adhesion, spreading, actin stress fiber formation, migration. The receptors are also critical mediators cellular mechanotransduction. In this contribution we assess whether novel class materials can the response cells to applied mechanical stimulation, using shear imparted by laminar fluid flow as a model...
Nitrofurantoin is a commonly used chemotherapeutic agent in the treatment of uncomplicated urinary tract infections caused by problematic multidrug resistant Gram-negative pathogen
Abstract The impact of geometrically constrained cis α,β‐unsaturated γ‐amino acids on the folding α,γ‐hybrid peptides was investigated. Structure analysis in single crystals and solution revealed that carbon–carbon double bonds can be accommodated into 12‐helix without deviation from overall helical conformation. structures are stabilized by 4→1 hydrogen bonding a similar manner to 12‐helices β‐peptides 3 10 helices α‐peptides. These results show functional backbone peptides.
Abstract Peptide macrocyclization is often a slow process, plagued by epimerization and cyclodimerization. Herein, we describe new method for peptide employing the Ag I ‐promoted transformation of thioamides. The has dual function: chemoselectively activating thioamide tethering N‐terminal to C‐terminal carboxylate. Extrusion 2 S generates an isoimide intermediate, which undergoes acyl transfer generate native cyclic peptide, resulting in rapid, traceless macrocylization process. Cyclic...
Abstract A facile synthesis of highly symmetrical tetrasubstituted pyrazines through simple aerial oxidation β‐keto γ‐amino esters is reported. The scope the reaction was examined by use various amino acid side‐chain functional groups and peptides. mild efficient transformation into may serve as an attractive strategy for self‐dimerization
Bicyclic analogues of celogentin C have been synthesized in which the side chain-side chain cross-links are replaced by thioether bonds. Several simplified bicyclic peptides displayed potent inhibition tubulin polymerization.