A5101839364- Prostate Cancer Treatment and Research
- PI3K/AKT/mTOR signaling in cancer
- Pancreatic function and diabetes
- Retinal Development and Disorders
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
- Sarcoma Diagnosis and Treatment
- Retinal Diseases and Treatments
- Estrogen and related hormone effects
- Pancreatitis Pathology and Treatment
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Metabolism, Diabetes, and Cancer
- Inflammatory mediators and NSAID effects
- Heat shock proteins research
- Mitochondrial Function and Pathology
- Colorectal and Anal Carcinomas
- Retinopathy of Prematurity Studies
- Ferroptosis and cancer prognosis
- Connexins and lens biology
- Cancer, Lipids, and Metabolism
- CRISPR and Genetic Engineering
Mayo Clinic in Florida
2018-2024
Nanchang University
2023-2024
Precision for Medicine (United States)
2023
Gansu Provincial Hospital
2023
East China Normal University
2020-2021
Tulane University
2016-2021
Guizhou Provincial People's Hospital
2021
Guizhou University
2021
Huazhong University of Science and Technology
2013-2020
Zhujiang Hospital
2020
Abstract Purpose: Intratumoral androgen synthesis (IAS) is a key mechanism promoting receptor (AR) reactivation and antiandrogen resistance in castration-resistant prostate cancer (CRPC). However, signaling pathways driving aberrant IAS remain poorly understood. Experimental Design: The effect of components the AKT-RUNX2-osteocalcin (OCN)–GPRC6A–CREB axis on expression steroidogenesis genes CYP11A1 CYP17A1 testosterone level were examined PTEN-null human cell lines. Pten knockout mice used...
Currently, an effective targeted therapy for pancreatitis is lacking. Hereditary (HP) a heritable, autosomal-dominant disorder with recurrent acute (AP) progressing to chronic (CP) and markedly increased risk of pancreatic cancer. In 1996, mutations in PRSS1 were linked the development HP. Here, we developed mouse model by inserting full-length human PRSS1R122H gene, most commonly mutated gene HP, into mice. Expression protein pancreas stress signaling pathways exacerbated AP. After attack...
Esophageal cancer is aggressive and has poor prognosis. squamous cell carcinoma (ESCC) histologically the most prevalent type of esophageal ranked as sixth leading cause death worldwide. In recent years, been widely regarded genetic disease, well epigenetic abnormalities including DNA methylation, histone deacetylation, chromatin remodeling, gene imprinting noncoding RNA regulation. this review, we will provide a general overview genes, proteins microRNAs that are involved in development...
Saturated fatty acids (SFAs) generally have been thought to worsen insulin-resistance and increase the risk of developing type 2 diabetes mellitus (T2DM). Recently, accumulating evidence has revealed that SFAs are not a single homogeneous group, instead different associated with T2DM in opposing directions. Pentadecanoic acid (C15:0, PA) is directly correlated dairy products, negative association between circulating PA metabolic disease was observed epidemiological studies. Therefore, role...
Abstract A poptosis r elated protein in T GF-β s ignaling pathway (ARTS) was originally discovered cells undergoing apoptosis response to TGF-β, but ARTS also acts downstream of many other apoptotic stimuli. induces by antagonizing the anti-apoptotic proteins XIAP and Bcl-2. Here we identified pro-apoptotic Sept4/ARTS gene as a p53-responsive target gene. Ectopic p53 variety p53-inducing agents increased both mRNA levels ARTS, whereas ablation reduced expression multiple stress conditions....
Retinitis pigmentosa (RP) affects about 1.8 million individuals worldwide. X-linked retinitis (XLRP) is one of the most severe forms RP. Nearly 85% XLRP cases are caused by mutations in 2 (RP2) and RPGR. RP2 has been considered to be a GTPase activator protein for ARL3 play role traffic ciliary proteins. The mechanism how cause RP still unclear. In this study, we generated an knockout zebrafish line using transcription activator-like effector nuclease technology. Progressive retinal...
The eukaryotic ribosome is essential for cancer cell survival. Perturbation of biogenesis induces nucleolar stress or ribosomal stress, which restrains growth, as rapidly proliferating cells need more active biogenesis. In this study, we found that UTP11 plays an important role in the biosynthesis 18S RNAs (rRNA) by binding to pre-rRNA processing factor, MPP10. overexpressed human cancers and associated with poor prognoses. Interestingly, depletion inhibits growth vitro vivo through...
Doxorubicin (DOX) is a potent available antitumor drug; however, its clinical use limited by the cardiotoxicity. Salidroside (SLD), with strong antioxidative and cytoprotective actions, of particular interest in development therapies for oxidative injury cardiac diseases. Now, protection underlying mechanisms SLD against DOX-induced cardiotoxicity are still unknown. In present study, we revealed both mechanism Bcl2-dependent survival signaling involved SLD's protection. We observed that DOX...
Abstract Objectives Enhancer of zeste homologue 2 ( EZH 2) is crucially involved in epigenetic silencing by acting as a histone methyltransferase. Although overexpressed many cancers and malignant cell proliferation invasion, the role senescence induced DNA damage has up to now remained largely unknown. In this study, we sought explore outcome depletion along with exposure doxorubicin DOX ), related mechanisms, gastric cancer cells. Materials methods Here, was achieved cells treatment....
Alisol A-24-acetate (AA-24-a) is one of the main active triterpenes isolated from well-known medicinal plant Alisma orientale (Sam.) Juz., which possesses multiple biological activities, including a hypoglycemic effect. Whether AA-24-a hypoglycemic-active compound A. Juz. unclear. The present study aimed to clarify effect and potential mechanism action on glucose uptake in C2C12 myotubes.Effects GLUT4 translocation plasma membrane were evaluated. Glucose was determined using...
Milk fat globule epidermal growth factor 8 (MFG-E8) is an opsonin involved in the phagocytosis of apoptotic cells. In patients with chronic obstructive pulmonary disease (COPD), cell clearance defective. However, whether aberrant MFG-E8 expression this defect unknown. study, we examined COPD patients. MFG-E8, interleukin (IL)-1β and transforming (TGF)-β levels were measured plasma 96 (93 males, 3 females; age range: 62.12±10.39) 87 age-matched healthy controls (85 2 64.81±10.11 years) using...
Tissue-specific gene manipulations are widely used in genetically engineered mouse models. A single recombinase system, such as the one using Alb-Cre, has been commonly for liver-specific genetic manipulations. However, most diseases complex, involving multiple changes and various cell types. dual system is required conditionally modifying different genes sequentially same or inducing types within organism. FlpO cDNA was inserted between last exon 3'-UTR of albumin a bacterial artificial...
A concise, environmentally benign, and cost-effective route was developed for the large-scale preparation of 1, a novel oxazolidinone antibacterial candidate. The key intermediate 2-(1-(2-fluoro-4-nitrophenyl)-1H-pyrazol-4-yl)pyridine 7 prepared with high purity by mild deamination regioisomeric mixture 21. from nucleophilic SNAr reaction selective C–N coupling secondary amine functionality 4-(pyridin-2-yl)-1H-pyrazol-3-amine 14 1,2-difluoro-4-nitrobenzene 10 in optimized conditions primary...
Thioredoxins (Trxs) are a class of small molecular redox proteins that play an important role in scavenging abnormally accumulated reactive oxygen species (ROS). Thioredoxin 2 (Trx2) is one member this family located mitochondria. Trx2 protects cells from increased oxidative stress and has anti-apoptosis function. Knockout mice led to early embryonic lethality. However, the essential during embryogenesis remains unclear. To further investigate development, we performed knockdown zebrafish...
Abstract Background Alisol A 24-acetate (AA-24-a), one of the main active triterpenes isolated from well-known medicinal plant Alisma orientale (Sam.) Juz., exhibits multiple biological activities including hypolipidemic activity. However, its effect on lipid metabolism in adipocytes remains unclear. The present study aimed to clarify AA-24-a adipocyte lipolysis and determine potential mechanism action using 3 T3-L1 cells. Methods We assayed release glycerol into culture medium cells under...
Hyperstimulation of the cholecystokinin 1 receptor (CCK1R), a G protein-coupled (GPCR), in pancreatic acinar cells is commonly used to induce pancreatitis rodents. Human lack CCK1R but express cholinergic muscarinic 3 (M3R), another GPCR. To test whether M3R activation involved pancreatitis, mutant was conditionally expressed mice. This loses responsiveness its native ligand, acetylcholine, can be activated by an inert small molecule, clozapine-N-oxide (CNO). Intracellular calcium and...