A5058696366- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Corneal surgery and disorders
- Barrier Structure and Function Studies
- Connexins and lens biology
- Neuroendocrine regulation and behavior
- Circadian rhythm and melatonin
- Neuroscience of respiration and sleep
- Glaucoma and retinal disorders
- Immune Response and Inflammation
- Nasal Surgery and Airway Studies
- Photoreceptor and optogenetics research
- S100 Proteins and Annexins
- Neurological Disease Mechanisms and Treatments
- Amoebic Infections and Treatments
- Advanced Glycation End Products research
- RNA Interference and Gene Delivery
- Ocular Diseases and Behçet’s Syndrome
- Cerebral Venous Sinus Thrombosis
- Infectious Encephalopathies and Encephalitis
- Neurofibromatosis and Schwannoma Cases
- Ocular Disorders and Treatments
- Systemic Lupus Erythematosus Research
- Intraocular Surgery and Lenses
- bioluminescence and chemiluminescence research
Kobe City Medical Center General Hospital
2025
Kyoto Prefectural University of Medicine
2009-2022
Nantan General Hospital
2002-2009
Yale University
1997-2000
To examine the feasibility of cultivated corneal endothelial cell transplantation in a primate model.Monkey cells (MCECs) obtained from three cynomolgus monkeys were cultivated, with subcultures grown on collagen type I carriers for 4 weeks. The endothelium right eye six was mechanically scraped, after which MCEC sheet brought into anterior chamber four eyes and fixed to Descemet's membrane by air. As control, without MCECs transplanted one monkey, suspension injected eye.Cultivated produced...
The combination of the tight junction transmembrane protein claudin subtypes is one most important determinants variations in tightness individual paired strands. barrier function corneal epithelium much stronger than that conjunctival epithelium. In this study, expression and cellular distribution species vivo human were investigated.Reverse transcription-polymerase chain reaction was used to reveal mRNA. Immunohistochemistry determine tissue junction-related proteins MUC5AC.Transcripts for...
Purpose: To review our attempt to devise a method of cultivated corneal endothelial transplantation using primates in which endothelium, like that humans, has low proliferative ability. Methods: Monkey cells (MCECs) were cultivated, with subcultures grown on collagen type I carriers. The endothelia 6 eyes monkeys scraped intensively, after MCEC sheets inserted into the anterior chamber 4 eyes. As controls, sheet without MCECs was transplanted 1 eye monkey, and suspension injected another...
Development of the daily rhythm vasoactive intestinal peptide (VIP) mRNA in rat suprachiasmatic nucleus (SCN), a main locus circadian oscillation, was investigated by situ hybridization. The phenotypic expression VIP neurons occurred two developmental stages ventrolateral portion SCN (VLSCN): first found before birth rostral part, and second part between postnatal day (P) 10 P20. latter period coincided with time that massive VIP-efferent fibers project to subparaventricular zone. In adult...
Subpial hemorrhage is a rare type of birth-related brain in neonates that can manifest with symptoms such as apnea and seizures. On magnetic resonance imaging (MRI), subpial parenchymal hemorrhages are typically observed but often resolve without lasting sequelae. Cytotoxic lesions the corpus callosum (CLOCCs) not common they adults children. We present case term neonate accompanied by CLOCCs who experienced favorable clinical course. A full-term female infant was delivered via emergency...
To identify the molecules involved in pathogenesis of gelatinous drop-like corneal dystrophy (GDLD) by using immunohistochemical analysis expression tight junction (TJ)-, desmosome-, and basement membrane (BM)-related proteins human corneas with GDLD.Mutation was performed on samples from three Japanese women GDLD. Four buttons these patients were examined histopathologically Congo red staining for TJ-related (ZO-1, occludin, claudin-1), desmosome components (desmoplakin), BM-related...
The retinal pigment epithelium (RPE) is an epithelial region of the blood-brain barrier. During embryogenesis, permeability barrier gradually decreases. A culture model RPE development revealed differences in how tight junctions regulate paracellular diffusion ionic and nonionic solutes (Ban Y Rizzolo LJ. Mol Vis 3: 18, 1997). To examine these differences, permeation monosaccharides was compared with mannitol, alkali metals sodium. order 3-O-methlyglucose = glucosamine mannitol >...
The suprachiasmatic nucleus (SCN) obtained from the newborn rat was successfully cultured in vitro for more than 4 weeks by roller tube method. slices which were 400μm thick and 1×1mm wide at time of extraction, became thinner wider week week, end weeks, had spread (2×2mm wide) flattened out to a monolayer or two-cell layers. culture composed three parts: ependymal zone consisting piled up cells central part, neuronal two tightly-packed cell masses located adjacent zone, surrounding glial...
The expression of peptidergic traits was analyzed in the slice culture suprachiasmatic nucleus (SCN) by roller tube technique. Since thickness one to three cell layers after weeks vitro, situ hybridization and immunocytochemistry could be applied directly slices without further cutting. We found peptides their messenger RNAs neurons neuronal zone occurring a topographic distinct pattern; vasopressin (AVP) containing dorsomedial part near ependymal vasoactive intestinal peptide (VIP)...
To evaluate the toxicity of Amphotericin B (AmB) in Optisol™-GS Corneal Storage Media (Bausch & Lomb) on corneal epithelial cell (CEC) morphology and migration ability.Sclerocorneal strips were removed from male Japanese white rabbits, then stored at 4 °C containing 0 µg/ml AmB (control group) 2.5, 5, 25, 50 (AmB groups; four eyes per group). After 7 days storage, CEC was evaluated by hematoxylin-eosin staining, immunohistochemical staining (ZO-1), terminal deoxynucleotidyl transferase dUTP...