purpose. The transplantation of cultivated corneal endothelial cells (CECs) has gained attention recently for the treatment patients with dysfunction. However, an efficient culturing technique human (H)CECs yet to be properly established. present study was conducted investigate applicability Rho kinase (ROCK) inhibitor Y-27632 in promoting cultivation cynomolgus monkey (M)CECs. methods. MCECs monkeys were cultured a medium containing 10 μM Y-27632. number viable adherent culture plates...

To evaluate the effect of Rho kinase (ROCK)-inhibitor eye drops on a corneal endothelial dysfunction primate model and human clinical case series dysfunction.As corneal-endothelial partially injured model, endothelium seven cynomolgus monkeys was damaged by transcorneal freezing; 10 mm rock inhibitor Y-27632 then applied topically 6 times daily. The phenotype reconstructed evaluated immunohistochemical analysis noncontact specular microscopy. For study, after freezing in eight patients: four...

Abstract The corneal endothelium maintains transparency; consequently, its dysfunction causes severe vision loss. Tissue engineering-based therapy, as an alternative to conventional donor transplantation, is anticipated provide a less invasive and more effective therapeutic modality. We conducted preclinical study for cell-based therapy in primate model demonstrated regeneration of the following injection cultured monkey endothelial cells (MCECs) or human CECs (HCECs), combination with Rho...

We examined the mechanisms underlying free carrier generation in a very topical PM6/Y6 organic solar cell. observed slow yet efficient spatial charge dissociation driven by downhill energy relaxation through interfacial cascade.

Magnetic resonance (MR) imaging was performed in 668 patients with chronic compressive lesions of the cervical spinal canal. High signal intensity observed within cord on T2-weighted or proton density spin-echo images 99 (14.8%). Frequency this finding directly proportional to severity clinical myelopathy and degree canal compression seen MR images. Patients a high-signal-intensity area responded less favorably than those without surgical medical treatment. More 60% had when grade moderate...

To examine the feasibility of cultivated corneal endothelial cell transplantation in a primate model.Monkey cells (MCECs) obtained from three cynomolgus monkeys were cultivated, with subcultures grown on collagen type I carriers for 4 weeks. The endothelium right eye six was mechanically scraped, after which MCEC sheet brought into anterior chamber four eyes and fixed to Descemet's membrane by air. As control, without MCECs transplanted one monkey, suspension injected eye.Cultivated produced...

<h3>Aim</h3> To demonstrate the efficacy of Rho-associated kinase (ROCK) inhibitor Y-27632 for corneal endothelial wound healing both in vitro and vivo models. <h3>Methods</h3> As an model, cultivated cynomolgus monkey cells were scraped to create a linear defect. The distance was then determined during 24-h culture presence or absence 10 μM Y-27632. central endothelium Japanese white rabbits damaged by transcorneal freezing, mM applied topically six times daily 48 h. area evaluated after...

Abstract Transient absorption spectroscopy (TAS) is a time‐resolved spectroscopic technique that can quantitatively observe temporal changes in the spectrum associated with transient species, such as excitons and charges. Therefore, it extensively used photophysics, photochemistry, photobiology. TAS plays crucial role unveiling charge generation recombination dynamics organic solar cells (OSCs). However, interpretation of TA data sometimes difficult for those who are not familiar TAS. In...

Dynamic magnetic resonance (MR) imaging with a 1.5-T superconductive unit was used in the evaluation of nine normal pituitary glands and 10 adenomas, including four microadenomas six macroadenomas. Seven to images were obtained every 20-30 seconds use spin-echo technique after rapid injection gadopentetate dimeglumine. The earliest contrast material enhancement structures seen infundibulum posterior lobe gland at 20 seconds, followed by gradual anterior from junction peripheral portion...

Chlorogenic acid, a dietary antioxidant, effectively inhibited the iron-induced lipid peroxidation of bovine liver microsomes in concentration-dependent manner. In Fenton-type reaction, chlorogenic acid production hydroxyl radical by iron-EDTA or iron-ADP, while iron plus did not generate radical. The formation an complex with was demonstrated UV/vis absorbance spectroscopic, ESR and 1H-NMR studies. ferric high-spin state near rhombicity, had no scavenging activity. results indicate that...

A total of 108 patients with ossification the posterior longitudinal ligament (OPLL) (n = 92), yellow (OYL) 8), or both 8) were examined magnetic resonance (MR) imaging performed 0.5-T superconductive and 0.22-T resistive units. OPLL was demonstrated as a low-signal-intensity band between bone marrow vertebral body dural sac on T1- T2-weighted images. Continuous cervical easier to diagnose than segmental OPLL. images more useful for detection ligaments. OYL recognized an impression sac....

Purpose: To review our attempt to devise a method of cultivated corneal endothelial transplantation using primates in which endothelium, like that humans, has low proliferative ability. Methods: Monkey cells (MCECs) were cultivated, with subcultures grown on collagen type I carriers. The endothelia 6 eyes monkeys scraped intensively, after MCEC sheets inserted into the anterior chamber 4 eyes. As controls, sheet without MCECs was transplanted 1 eye monkey, and suspension injected another...

Corneal endothelial cells (CECs) show poor regenerative ability in humans, and noncompensatory damage of CECs causes irreversible corneal haziness cases bullous keratopathy. Although transplantations provide considerable clinical benefits, allograft rejection, primary graft failure, the shortage donor corneas are problems that still need to be overcome. The establishment new treatment therapies is key solving these problems, we have attempted establish a intervention for dysfunction. We...

This paper presents a calculation method of computer-generated holograms that involves removing the hidden surface and provides realistic rendering. The was based on ray-tracing simulates rays traveling paths. Rays are cast from every elementary hologram into virtual objects then paths determined. Since is considering intersection with objects, absorption, reflection, refraction, capable rendering images. Multiple reflections refraction expressed by casting additional reflection direction...

Understanding the excited-state dynamics of nonfullerene electron acceptors is essential for further improvement organic solar cells as they are responsible near-IR light absorption. Herein, we investigated singlet and triplet in Y6, a novel acceptor, using transient absorption spectroscopy. We found that, even at low excitation fluences, pristine Y6 films show biphasic exciton decay kinetics with constants ∼220 ps ∼1200 ps. The majority excitons decayed faster (∼220 ps) component, whereas...

There have been many studies on navigation systems in cislunar space. Many proposed a special system to be constructed around the moon, similar Global Navigation Satellite System (GNSS) Earth. In recent years, authors presented new method for providing simultaneous range measurement and clock synchronization. This paper presents experimental results performed two field radio tests using actual hardware. study aimed demonstrate GNSS–based transceiver communication capability built developed...

Thermorechargeable battery (TB), which can be charged by temperature change Δ via difference in the electrochemical Seebeck coefficient α between cathode and anode, is a promising energy harvester. To...

Chlorogenic acid prevented a stepwise conversion of plasmid pUC18 DNA, from I-->form II-->form III, induced by 3 mM monochloramine with half inhibition 67.4 microM. reacted in time-dependent manner, and the reaction rate increased decreasing pH. These results suggest that chlorogenic prevents genotoxicity gastric mucosa.

Seventy-six patients with persistent myelopathy secondary to chronic spinal cord injuries underwent examination magnetic resonance (MR) imaging, the results of which were correlated neurologic findings. Twenty-one received follow-up study during and after acute stage; 55 examined only at a stage. Spinal abnormalities seen in 48 according five patterns: (a) normal signal intensity on T1- T2-weighted images (pattern N/N, n = 28), (b) T1-weighted hyperintensity N/Hi, 18), (c) hypointensity...

Computer-generated holograms (CGHs) are usually displayed on electronic devices. However, the resolution of current output devices is not high enough to display CGHs, so visual field very narrow. A method using a Fourier transform optical system has been proposed, enlarge size reconstructed images. This paper describes CGH calculations for and reconstruct realistic images by ray tracing method. reconstructs at arbitrary depths also eliminates unnecessary light including zero-th order light.