A5083496550- Mycobacterium research and diagnosis
- Infectious Diseases and Mycology
- Tuberculosis Research and Epidemiology
- Pharmacological Effects of Natural Compounds
- Fungal Infections and Studies
- Infectious Diseases and Tuberculosis
- Vibrio bacteria research studies
- Veterinary medicine and infectious diseases
- Antibiotic Resistance in Bacteria
- Plant Pathogenic Bacteria Studies
- Syphilis Diagnosis and Treatment
- Actinomycetales infections and treatment
- Humic Substances and Bio-Organic Studies
- Dermatological diseases and infestations
- Ginseng Biological Effects and Applications
- Quinazolinone synthesis and applications
- Parasites and Host Interactions
- Fungal Biology and Applications
- Pneumonia and Respiratory Infections
- Escherichia coli research studies
- Gut microbiota and health
- Medicinal Plant Pharmacodynamics Research
- Cancer-related molecular mechanisms research
- Biological Control of Invasive Species
- HIV/AIDS oral health manifestations
Institut Bergonié
2022-2025
Inserm
2015-2024
Université d'Angers
2015-2024
Nantes Université
2012-2024
Centre National de la Recherche Scientifique
2014-2024
Centre Hospitalier Universitaire d'Angers
2012-2024
Immunologie et Nouveaux Concepts en Immunothérapie
2022-2023
London School of Hygiene & Tropical Medicine
2022
Hospital for Tropical Diseases
2022
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2012-2021
Buruli ulcer is a neglected tropical disease caused by Mycobacterium ulcerans infection that damages the skin and subcutis. It most prevalent in western central Africa Australia. Standard antimicrobial treatment with oral rifampicin 10 mg/kg plus intramuscular streptomycin 15 once daily for 8 weeks (RS8) highly effective, but injections are painful potentially harmful. We aimed to compare efficacy tolerability of fully clarithromycin extended release (RC8) RS8 early lesions.
Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by activating RNA-programmable RNases and DNases. Cas13a Cas12a enzymes bound to RNA (crRNA) recognize specific acid targets, initiating cleavage of the targets as well non-target (trans) acids. Here, we examine kinetics single-turnover target multi-turnover trans-nuclease activities both enzymes. High-turnover, non-specific trans-RNase activity is coupled rapid binding RNA. By contrast, low-turnover relatively...
Buruli ulcer, the third mycobacterial disease after tuberculosis and leprosy, is caused by environmental mycobacterium M. ulcerans. Various modes of transmission have been suspected for this disease, with no general consensus acceptance any them up to now. Since laboratory models demonstrated ability water bugs transmit ulcerans, a particular attention focused on bacilli as hosts vectors. However, it only through detailed knowledge biodiversity ecology that importance mode can be fully...
Mycolactone, a lipid-like toxin, is the major virulence factor of Mycobacterium ulcerans , etiological agent Buruli ulcer. Its involvement in lesion development has been widely described early stages disease, through its cytotoxic and immunosuppressive activities, but less known about later stages. Here, we revisit role mycolactone disease outcome provide first demonstration pro-inflammatory potential this toxin. We found that mycolactone-containing mycobacterial extracellular vesicles...
Aims Leiomyomas (LM) are the most common uterine mesenchymal neoplasms and encompass a variety of histological subtypes. Bizarre nuclei described in both leiomyomas with bizarre (LM‐BN) fumarate hydratase‐deficient (FH‐LM), which raise diagnostic concerns regarding leiomyosarcoma (LMS). Recently, an immunohistochemical algorithm to support diagnosis LMS based on genomic landscape these was proposed. This study aimed evaluate algorithm's accuracy distinguishing LM‐BN FH‐LM from LMS. Methods...
Mycolactone is a lipid-like endotoxin synthesized by an environmental human pathogen, Mycobacterium ulcerans, the causal agent of Buruli ulcer disease. has pleiotropic effects on fundamental cellular processes (cell adhesion, cell death and inflammation). Various targets mycolactone have been identified literature survey revealed that most these are membrane receptors residing in ordered plasma nanodomains, within which their functionalities can be modulated. We investigated capacity to...
Introduction Mycobacterium ulcerans infection, known as Buruli ulcer, is a disease of the skin and subcutaneous tissues which an important but neglected tropical with its major impact in rural parts West Central Africa where facilities for diagnosis management are poorly developed. We evaluated fluorescent thin layer chromatography (f-TLC) detection mycolactone laboratory using samples from patients ulcer similar lesions that gave negative result on PCR IS2404 repeat sequence M....
Buruli ulcer (BU) is an insidious neglected tropical disease. Cases are reported around the world but rural regions of West and Central Africa most affected. How BU transmitted spreads has remained a mystery, even though causative agent, Mycobacterium ulcerans, been known for more than 70 years. Here, using tools population genomics, we reconstruct evolutionary history M. ulcerans by comparing 165 isolates spanning 48 years representing 11 endemic countries across Africa. The genetic...
Abstract Buruli ulcer, a debilitating disease, is caused by Mycobacterium ulcerans. The incidence of this neglected tropical disease steadily increasing. As rule, without treatment, skin ulcers occur and lengthy healing process may be observed associated with severe functional disabilities. Mouse models are already available to study establishment lesions or evaluation therapy but lack suitable animal model, mimicking all clinical stages, in particular the process, remains an obstacle...
Mycolactone A/B is a lipophilic macrocyclic polyketide that the primary virulence factor produced by Mycobacterium ulcerans, human pathogen and causative agent of Buruli ulcer. In M. ulcerans strain Agy99 mycolactone synthase (PKS) locus spans 120 kb region 174 megaplasmid. Here we have identified promoter regions this PKS using GFP reporter assays, in silico analysis, primer extension, site-directed mutagenesis. Transcription large genes mlsA1 (51 kb), mlsA2 (7 kb) mlsB (42 driven novel...
Mycolactones are toxins secreted by M. ulcerans, the etiological agent of Buruli ulcer. These toxins, which main virulence factors bacilli, responsible for skin lesions. Considering their specificity ulcerans and presence in lesions even at early stages, mycolactones promising candidates development a diagnostic tool infection. Stability purified towards light heat has not yet been investigated, despite importance such parameters selection strategies diagnosis development. In this context,...
The occurrences of many environmentally-persistent and zoonotic infections are driven by ecosystem changes, which in turn underpinned land-use modifications that alter the governance pathogen, biodiversity human interactions. Our current understanding these ecological changes on disease emergence however remains limited. Buruli ulcer is an emerging skin caused mycobacterium, Mycobacterium ulcerans, for exact route infection unclear. It can have a devastating impact its host, causing...
Background Buruli ulcer, the third mycobacterial disease after tuberculosis and leprosy, is caused by environmental mycobacterium M. ulcerans. There at present no clear understanding of exact mode(s) transmission Populations affected ulcer are those living close to humid swampy zones. The associated with creation or extension areas, such as construction dams lakes for development agriculture. Currently, it supposed that insects (water bugs mosquitoes) host vector role water was clearly...
Mycolactone, a polyketide molecule produced by Mycobacterium ulcerans, is the etiological agent of Buruli ulcer. This lipid toxin endowed with pleiotropic effects, presents cytotoxic effects at high doses, and notably plays pivotal role in host response upon colonization bacillus. Most remarkably, mycolactone displays intriguing analgesic capabilities: suppresses or alleviates pain skin lesions it inflicts. We demonstrated that capability was not attributable to nerve damage, but instead...
Background Mycobacterium ulcerans, a slow-growing environmental bacterium, is the etiologic agent of Buruli ulcer, necrotic skin disease. Skin lesions are caused by mycolactone, main virulence factor M. with dermonecrotic (destruction and soft tissues) immunosuppressive activities. This toxin secreted in vesicles that enhance its biological Nowadays, it well established reservoir bacilli localized aquatic environment where bacillus may be able to colonize different niches. Here we report...
Background. Mycobacterium ulcerans is known to cause Buruli ulcer (BU), a necrotizing skin disease leading extensive cutaneous and subcutaneous destruction functional limitations. However, M. infections are not limited skin, osteomyelitis, still poorly described in the literature, occurs numerous young patients Africa.
Background. Buruli ulcer, caused by Mycobacterium ulcerans, is a necrotizing skin disease leading to extensive cutaneous and subcutaneous destruction functional limitations. Spontaneous healing in the absence of medical treatment occurs rare cases, but this has not been well described literature. Methods. In retrospective case study an area Benin where highly endemic, we selected 26 ulcer patients presenting features spontaneous from cohort 545 treated between 2010 2013. Results. The studied...
Nigeria is known to be endemic Buruli ulcer, but epidemiological data are remarkably rare. Here, we present a large cohort of 127 PCR-confirmed M. ulcerans infection patients coming from and treated in neighbouring country, Benin. Severe lesions delay consultation factors that should encourage establishment treatment centre South Western Nigeria.
Bacterial transcriptome analyses during host colonization are essential to decipher the complexity of relationship between bacterium and its host. RNA sequencing (RNA-seq) is a promising approach providing valuable information about bacterial adaptation, response and, in some cases, mutual tolerance underlying crosstalk, as recently observed context Mycobacterium ulcerans infection. Buruli ulcer caused by M. ulcerans. This neglected disease third most common mycobacterial worldwide. Without...
Abstract Buruli ulcer, caused by Mycobacterium ulcerans and characterized devastating necrotizing skin lesions, is the third mycobacterial disease worldwide. The role of host genetics in susceptibility to ulcer has long been suggested. We conduct first genome-wide association study on a sample 1524 well patients controls from rural Benin. Two-stage analyses identify two variants located within LncRNA genes: rs9814705 ENSG00000240095.1 ( P = 2.85 × 10 −7 ; odds ratio 1.80 [1.43–2.27]),...
Background Gut colonization by ESBL-producing Enterobacteriaceae (ESBL-PE) is widespread and promoted antibiotic exposure. Higher fecal abundance of ESBL-PE promotes the dissemination bacteria in environment associated with increased risk infection. Ceftriaxone temocillin are commonly used antibiotics a different activity on gut flora. Their impact has not been studied. The objective this study was to compare propensity ceftriaxone modify Escherichia coli feces colonized mice. Methods Mice...
Buruli ulcer is one of the 20 neglected tropical diseases in world. This necrotizing hypodermitis a chronic debilitating disease caused by an environmental Mycobacterium ulcerans . At least 33 countries with tropical, subtropical and temperate climates have reported African countries, South America Western Pacific regions. Majority cases are spread across West Central Africa. The mode transmission unclear, hindering implementation adequate prevention for population. Currently, early...