A5100387677- Parasites and Host Interactions
- Helminth infection and control
- Parasite Biology and Host Interactions
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Animal Disease Management and Epidemiology
- Genomic variations and chromosomal abnormalities
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- Cytokine Signaling Pathways and Interactions
- Vector-Borne Animal Diseases
- Cancer-related gene regulation
- Coccidia and coccidiosis research
- Monoclonal and Polyclonal Antibodies Research
- Genetic factors in colorectal cancer
- Viral Infections and Immunology Research
- interferon and immune responses
- Parasitic Infections and Diagnostics
- RNA and protein synthesis mechanisms
Huazhong Agricultural University
2017-2025
Center of Hubei Cooperative Innovation for Emissions Trading System
2023
Current research on common parasitic nematodes is limited because their infective stages cannot be propagated in vitro. Here, we report a culture system for developing L4s of Haemonchus contortus, blood-feeding nematode ruminants. Our results demonstrated that proportionate mixture NCTC-109 to Luria-Bertini (1:2) media promoted the formation early and then into late upon inclusion 12.5% (v/v) defibrinated blood, albeit with decline survival. Adding antioxidants (0.3 mg/mL L-glutathione or...
Barbervax is the first and only available vaccine to protect animals against Haemonchus contortus - one of most pathogenic parasites small ruminants. This contains a kind native antigen called H11, glycoprotein complex derived from integral gut this parasite. Native H11 has been shown induce high levels (72-95%) protection, but single or two recombinant molecules are consistently unsuccessful. An increasing number aminopeptidases related have characterized in past three decades, little known...
Abstract Background Ivermectin (IVM) is one of the most important and widely used anthelmintics in veterinary medicine. However, its efficacy increasingly compromised by widespread resistance, exact mechanism IVM resistance remains unclear for parasitic nematodes, including Haemonchus contortus , a blood-sucking nematode small ruminants. Methods In this study, an H. IVM-resistant strain from Zhaosu, Xinjiang, China, was isolated assessed control test, faecal egg count reduction test (FECRT)...
African swine fever virus (ASFV) infection is a major public and socioeconomic concern that has serious impact on the global industry. Unfortunately, there are currently no commercially available vaccines or antiviral agents both safe effective against ASFV. In study, we use primary porcine alveolar macrophages to screen kinase inhibitor library for anti-ASFV compounds. Six candidate compounds inhibited ASFV with inhibition of > 90% were identified, among which brincidofovir exhibited...
Citrus sinensis is the most cultivated and economically valuable species in world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to small size of chromosomes, difficulty differentiating between pseudo real chromosomes because highly heterozygous genome. Here, we employ fluorescence situ hybridization (FISH) with 9 painting probes, 30 oligo pools, 8 repetitive sequences visualize 18 chromosomes. Then, develop an approach...
The transforming growth factor beta (TGF-β) pathway has been implicated in carcinogenesis of intestinal canal. Except for common variants indentified by genome-wide association studies, with lower frequency can also explain a part the disease heritability, especially those gene regulatory regions. In this study, we searched colorectal cancer (CRC) related functional low-frequency (minor allele 1-5%) untranslated regions (UTR) involved TGF-β signaling using next-generation sequencing based...
Janus kinase 3 (JAK3) plays a critical role in the JAK/STAT signaling pathway and has become an attractive selective target for treatment of immune-mediated disorders. Therefore, great efforts have been made development JAK3 inhibitors, but developing inhibitors remains challenge because high sequence homology with other kinases. In order to reveal selective-binding mechanisms find key structural features that refer specific inhibition, systematic computational method, including 3D-QSAR,...
Gastrointestinal nematodes (GINs) infect sheep, goats and cattle, causing huge economic losses to the breeding industry worldwide. The current major control method is usage of anthelmintic drugs, however, widespread issue parasite resistance means that this approach becoming unsustainable. Vaccination has been regarded as a long-term sustainable intervention strategy for controlling these parasitoses. In past several decades, substantial progress made in developing vaccines against GINs...
Abstract Centromeres play a crucial role in ensuring the accurate separation of chromosomes during cell division. Despite three rounds genome sequencing technology undergone by Citrus sinensis (sweet orange), presence numerous repetitive DNA elements its has led to substantial gaps centromeric genomic mapping, leaving composition repeats unclear. To address this, we employed combination chromatin immunoprecipitation with C. centromere-specific histone H3 variant antibody and bacterial...
Abstract Background In most multicellular organisms, the transforming growth factor-β (TGF-β) signalling pathway is involved in regulating and stem cell differentiation. Previous studies have demonstrated importance of three key molecules this parasitic nematode Haemonchus contortus , including one TGF-β type I receptor ( Hc-tgfbr1 ), II Hc-tgfbr2 co-Smad Hc-daf-3 which regulated developmental transition from free-living to stages parasite. However, almost nothing known about function ligand...
Most haematophagous organisms constantly suck the host's haemoglobin, which produces toxic free haem. This haem aggregation into nontoxic crystallisation complex known as haemozoin represents one of most important detoxification pathways in living organisms, but very little is about features parasitic nematodes. Here, we identified and characterised an economically significant blood-sucking nematode, Haemonchus contortus.Using electron microscopy, spectrophotometry analyses biochemical...
The CAP superfamily proteins are distributed widely in eukaryotes and play crucial roles various biological processes. However, very little is known about their functions parasitic nematodes, including Haemonchus contortus, a socioeconomically important nematode. We have therefore studied member of the protein family H. named Hc-CAP-15, with aim to explore its regulating developmental process. conservation phylogenetic relationships, spatial expression temporal transcription profiles...
Abstract Background The majority of hematophagous organisms constantly suck the host’s hemoglobin that produces toxic free haem. This haem aggregation into nontoxic crystallization complex known as hemozoin, which represents one most important detoxification pathways in living organisms, but very little is about features haemozoin parasitic nematodes. Here, we identified and characterized an economically significant blood-sucking nematode, Haemonchus contortus . Methods Using electron...
Abstract Background In most multicellular organisms, transforming growth factor-β (TGF-β) signalling pathway is involved in regulating the and stem cell differentiation. Previous studies have demonstrated importance of three key molecules this parasitic nematode Haemonchus contortus , including one TGF-β type I receptor ( Hc-tgfbr1 ), II Hc-tgfbr2 co-Smad Hc-daf-3 which regulated developmental transition from free-living to stages parasite. However, almost nothing known about function ligand...