A5054854639- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Slime Mold and Myxomycetes Research
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Resistance in Bacteria
- Bacillus and Francisella bacterial research
- Biochemical and Structural Characterization
- Gut microbiota and health
- Microbial Metabolic Engineering and Bioproduction
- Brucella: diagnosis, epidemiology, treatment
- Cancer Research and Treatments
- Microbial Inactivation Methods
- Escherichia coli research studies
- Legionella and Acanthamoeba research
- Parasitic Infections and Diagnostics
- Plant and Biological Electrophysiology Studies
Centre National de la Recherche Scientifique
2007-2022
Inserm
2022
Institut de Biologie de l'École Normale Supérieure
2022
University of Namur
2011
Université Joseph Fourier
2006-2008
Translational Innovation in Medicine and Complexity
2008
Université Grenoble Alpes
2007
Centre Hospitalier Universitaire de Grenoble
2006
Bacteria of the Brucella genus are facultative intracellular class III pathogens. These bacteria able to control trafficking their vacuole, presumably by use yet unknown translocated effectors. To identify such effectors, we used a high-throughput yeast two-hybrid screen interactions between putative human phagosomal proteins and predicted spp. proteins. We identified specific interaction small GTPase Rab2 protein named RicA. This was confirmed GST-pull-down with GDP-bound form Rab2. A...
The type III secretion system (TTSS) of Pseudomonas aeruginosa is induced in vivo upon contact with eukaryotic cells and vitro by calcium depletion culture medium. We have observed a previously identified protein, PsrA, necessary for full activation TTSS gene expression P. aeruginosa. Electrophoretic mobility shift assays showed that recombinant PsrA could bind to the exsCEBA promoter region. A mutant deletion psrA was constructed. Using transcriptional fusions, we demonstrated required...
Immunotherapy requiring an efficient T lymphocyte response is initiated by antigen delivery to antigen-presenting cells. Several studies have assessed the efficiency of various loading procedures, including microbial vectors. Here a live strain Pseudomonas aeruginosa was engineered translocate recombinant antigenic protein into mammalian cells via type III secretion system, bacterial device translocating effector proteins host Optimization vector included virulence attenuation and...
The Pseudomonas aeruginosa type III secretion system (T3SS) is known to be a very important virulence factor in acute human infections, but it less maintaining chronic infections which T3SS genes are downregulated. In vitro, the activation of expression involves positive activating loop that acts on transcriptional regulator ExsA. We have observed vivo cell density-dependent manner does not need quorum-sensing (QS) signals. addition, stationary-phase culture supernatants added...
ABSTRACT During the last few years, use of type III secretion system-based bacterial vectors for immunotherapy purposes has been assessed in various applications. We showed that a secretion-based Pseudomonas aeruginosa vector delivering ovalbumin (OVA) antigen induced an efficient specific CD8 + T-lymphocyte immune response against OVA-expressing cells. Because intrinsic toxicity vector, further virulence attenuation was needed. Therefore, we explored effects deletion quorum-sensing genes...
Abstract Background Pseudomonas aeruginosa , an opportunistic pathogen, is often encountered in chronic lung diseases such as cystic fibrosis or obstructive pneumonia, well acute settings like mechanical ventilation acquired pneumonia neutropenic patients. It a major cause of mortality and morbidity these diseases. In lungs, P. settles biofilm mode growth with the secretion exopolysaccharides which it encapsulated, enhancing its antibiotic resistance contributing to respiratory deficiency...
Abstract RNA silencing is a gene mechanism directed by small RNAs. Human miRNAs act as central regulators of host-bacteria interactions. However, it unknown whether human pathogenic bacteria could impede to promote virulence. Here, we show that the Legionella pneumophila type IV-secreted effector LegK1 efficiently suppresses siRNA and miRNA activities in cells. This effect depends on its known kinase activity, but also novel capacity, found here, bind Argonaute (Ago) proteins. We further...