A5064490338- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Immunotherapy and Immune Responses
- Peptidase Inhibition and Analysis
- Dendrimers and Hyperbranched Polymers
- Bacteriophages and microbial interactions
- Proteoglycans and glycosaminoglycans research
- Galectins and Cancer Biology
- RNA and protein synthesis mechanisms
- Drug Transport and Resistance Mechanisms
- Crystallization and Solubility Studies
- Electrochemical sensors and biosensors
- Advanced Biosensing Techniques and Applications
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Synthesis of Organic Compounds
- Peanut Plant Research Studies
- X-ray Diffraction in Crystallography
- Coconut Research and Applications
Université Grenoble Alpes
2015-2024
Département de Chimie Moléculaire
2014-2024
Centre National de la Recherche Scientifique
2013-2023
Institut Universitaire de France
2012-2017
Université Joseph Fourier
2005-2014
University of California, Irvine
2010-2014
University of Florence
2014
Institut des Biomolécules Max Mousseron
2014
Institut de Chimie Moléculaire de Grenoble
2005-2013
CIC biomaGUNE
2011
Metal overload plays an important role in several diseases or intoxications, like Wilson's disease, a major genetic disorder of copper metabolism humans. To efficiently and selectively decrease concentration the liver that is highly damaged, chelators should be targeted at hepatocytes. In present work, we synthesized molecule able to both lower intracellular copper, namely Cu(I), target hepatocytes, combining within same structure chelating unit carbohydrate recognition element. A...
A new generation of synthetic cancer vaccine: the first self-adjuvanting vaccine prototype combining a cluster B cell epitope, CD4+ T helper CD8+ and an immunoadjuvant has been synthesized by chemoselective strategy. Vaccination mice with this molecularly defined construction induces strong protection against tumors. Supporting information for article is available on WWW under http://www.wiley-vch.de/contents/jc_2452/2008/z700315_s.pdf or from author. Please note: The publisher not...
We report the highly efficient preparation of well-defined tetravalent glycoconjugates as potential tools for cell targeting and cell-surface mimics. Our strategy is based on chemoselective assembly aminooxy carbohydrates to a cyclic decapeptide template presenting aldehydes, namely, "regioselectively addressable functionalized template" (RAFT). Preliminary recognition assays tetramannosyl glycoconjugate (20) with specific lectin (Concanavalin A) using fluorescence anisotropy method are also...
In this paper we investigated the use of regioselectively addressable functionalized templates (RAFTs) as new scaffolds for design anticancer vaccine candidates. We report synthesis well-defined multiepitopic RAFT and their immunological evaluation. These conjugates exhibit clustered Tn analogue tumor-associated carbohydrate antigen (TACA, B-cell epitope) CD4+ helper T-cell peptide from type 1 poliovirus. The saccharidic peptidic epitopes were both synthesized separately combined to core...
X-ray crystallography studies, as well live-cell fluorescent imaging, have recently challenged the traditional view of protein kinase CK2. Unbalanced expression catalytic and regulatory CK2 subunits has been observed in a variety tissues tumours. Thus potential intersubunit flexibility suggested by these studies raises likely prospect that holoenzyme complex is subject to disassembly reassembly. In present paper, we show evidence for reversible multimeric organization vitro. We used...
A 15 625-membered peptide dendrimer combinatorial library was acylated with an α-C-fucosyl residue at its four N-termini and screened for binding to fucose-specific lectins. Dendrimer FD2 (Fuc-α-CH2CO-Lys-Pro-Leu)4(Lys-Phe-Lys-Ile)2Lys-His-Ile-NH2 identified as a potent ligand against Ulex europaeus lectin UEA-I (IC50 = 11 μM) Pseudomonas aeruginosa PA-IIL 0.14 μM).
Glyco-lipopeptides, a form of lipid-tailed glyco-peptide, are currently under intense investigation as B- and T-cell based vaccine immunotherapy for many cancers. However, the cellular molecular mechanisms glyco-lipopeptides (GLPs) immunogenicity position lipid moiety on protective efficacy GLPs remain to be determined.
Abstract Tumor‐associated carbohydrate antigens (TACAs) are key components of cancer vaccines. A variety vaccines based on native TACAs such as α‐Tn have shown immunogenicity and protection in preclinical animal studies, however, their weak immunogenicity, low vivo instability, poor bioavailability, discouraged further evaluations clinical studies. new improved vaccine prototype is reported. It composed four clustered Tn‐antigen mimetics a immunogenic peptide epitope that conjugated to...
QCM-D is demonstrated as a novel method to quantify the reaction yields and stability of reducing-end conjugation glycosaminoglycans.
Two series of multivalent octasilsesquioxane glyco- and peptido-conjugates were synthesized using the photoinduced free-radical thiol–ene coupling (TEC). The first was obtained by C-glycosylpropyl thiols cysteine containing peptides with known octavinyl while second reacting glycosyl a new derivative displaying eight PEGylated chains functionalized terminal allyl groups. evaluation binding properties mannoside glucoside clusters toward Concanavalin A Enzyme-Linked Lectin Assay (ELLA)...
A novel strategy to prepare glycopeptide−oligonucleotide conjugates bearing a glycocluster is reported. The utilizes cyclodecapeptide scaffold as key intermediate anchor the carbohydrate cluster and oligonucleotide through sequential oxime bond formation. retains binding affinity recognition specificity for target sequence. Furthermore, conjugate shows enhanced specific lectins due cooperative effect produced by cluster.
Following an iterative oxime ligation procedure, cyclopeptide (R) and lysine-based dendron (D) were combined in all possible arrangements successively functionalized with α-fucose β-fucose to provide a new series of hexadecavalent glycosylated scaffolds (i.e., RD16, RR16, DR16, DD16). These compounds smaller analogs (tetra- hexavalent R4 R6) used evaluate the influence ligand valency architecture, anomer configuration binding αFuc-specific lectin LecB from Pseudomonas aeruginosa ....
Abstract Liver cells are an essential target for drug delivery in many diseases. The hepatocytes express the asialoglycoprotein receptor (ASGPR), which promotes specific uptake by means of N‐acetylgalactosamine (GalNAc) recognition. In this work, we designed two different chemical architectures to treat Wilson's disease intracellular copper chelation. Two glycoconjugates functionalized with three or four GalNAc units each were shown enter hepatic and chelate copper. Here, studied series...
Here we described C-type lectin-oriented surfaces for SPR analysis. They allow the preservation of receptor topology, accessibility binding sites, better evaluation high avidity compounds and assessment multivalent effect at cell surface.
The investigation of recognition events between carbohydrates and proteins, especially the understanding how spatial factors binding avidity are correlated, remains a great interest for glycobiology. In this context we have investigated by nanogravimetry (QCM-D) surface plasmon resonance (SPR), kinetics thermodynamics interaction concanavalin A (Con A) various neoglycopeptide ligands low molecular weight. Regioselectively addressable functionalized templates (RAFT) been used as scaffolds...
A series of tetravalent glycocyclopeptides functionalized with GlcNAc was synthesized using copper(I)-catalysed alkyne–azide cycloaddition, oxime ligation and thiol–ene coupling. The binding ability these compounds towards wheat germ agglutinin studied by a competitive ELLA test ITC experiments. While all were able to inhibit WGA GlcNAc-polymer coated surfaces at low concentrations, derivative 17 having an aliphatic spacer thioether linkage 4.9 × 106 times more potent on per sugar basis than...