A5053175912- Bacterial Genetics and Biotechnology
- Gene Regulatory Network Analysis
- Microbial Metabolic Engineering and Bioproduction
- Rabies epidemiology and control
- CRISPR and Genetic Engineering
- Viral Infections and Outbreaks Research
- Olfactory and Sensory Function Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microbial infections and disease research
- bioluminescence and chemiluminescence research
- Ionic liquids properties and applications
- Hepatitis B Virus Studies
- Mental Health and Patient Involvement
- Acne and Rosacea Treatments and Effects
- Nuclear reactor physics and engineering
- Phenothiazines and Benzothiazines Synthesis and Activities
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Nuclear Materials and Properties
- Antimicrobial agents and applications
- Nuclear Physics and Applications
- Allergic Rhinitis and Sensitization
- Health Policy Implementation Science
- Contact Dermatitis and Allergies
- Protein Structure and Dynamics
Los Alamos National Laboratory
2014-2023
Karolinska Institutet
2023
Karolinska University Hospital
2023
Portland Public Schools
1991
Significance Effective treatment of skin-based bacterial biofilms has been identified as a serious and unmet medical need. Biofilm-protected bacteria account for ∼80% infections in humans are 50–1,000 times more resistant to antibiotics than their planktonic counterparts. Biofilms skin further protected by the outermost layer skin, stratum corneum, which serves natural barrier most therapeutics. Here, we present compelling evidence exploiting ionic liquids (ILs) an arsenal materials both...
Antiseptic agents are the primary arsenal to disinfect skin and prevent pathogens spreading within host as well into surroundings; however Food Drug Administration published a report in 2015 requiring additional validation of nearly all current antiseptic before their continued use can be allowed. This vulnerable position calls for urgent identification novel agents. Recently, ability deep eutectic, Choline And Geranate (CAGE), treat biofilms Pseudomonas aeruginosa Salmonella enterica was...
Licensed rabies virus vaccines based on whole inactivated are effective in humans. However, there is a lack of detailed investigations the elicited immune response, and whether responses can be improved using novel vaccine platforms. Here we show that two doses lipid nanoparticle-formulated unmodified mRNA encoding glycoprotein (RABV-G) induces higher levels RABV-G specific plasmablasts T cells blood, plasma bone marrow compared to Rabipur non-human primates. The also generates binding...
We created a single cell sorting system to screen for enzyme activity in Escherichia coli producing 3,4 dihydroxy benzoate (34DHB). To do so, we engineered transcription factor regulon controlling the expression of green fluorescent protein (GFP) induction by 34DHB. An autoregulated factor, pcaU, was borrowed from Acinetobacter sp ADP1 E. and its promoter region adapted Coli. The pcaU inducible at >5 μM exogenous 34DHB, making it sensitive biosensor this industrially significant nylon...
ABSTRACT Structure‐based rational mutagenesis for engineering protein functionality has been limited by the scarcity and difficulty of obtaining crystal structures desired proteins. On other hand, when high‐throughput selection is possible, directed evolution‐based approaches gaining functionalities have random fortuitous with rationalization. We combine comparative modeling dimer structures, ab initio loop reconstruction, ligand docking to select positions create a library focused on...
To identify high productivity strains for microalgal biofuels generation, the maximum specific growth rate of 38 was measured as a function salinity (i.e., 5, 15, and 35 PSU) temperature at 8 temperatures along linear gradient from ca. 5 to 45 °C) determine most suitable medium best growing season, respectively, outdoor raceway pond cultivation. The following were evaluated: Agmenellum quadruplicatum UTEX 2268, Anabaena sp. ATCC 33081, Arthrospira fusiformis 2721, platensis 3086, Chlorella...
A whole-cell biosensor utilizing a transcription factor (TF) is an effective tool for sensitive and selective detection of specialty chemicals or anthropogenic molecules, but requires access to expanded repertoire TFs. Using homology modeling ligand docking binding pocket identification, assisted by conservative mutations in the pocket, we engineered novel specificity Acinetobacter TF, PobR, 'sense' chemical p-nitrophenol (pNP) measured response via fluorescent protein reporter expressed...
Robust fluorescence-based biosensors are emerging as critical tools for high-throughput strain improvement in synthetic biology. Many developed model organisms where sophisticated biology also well established. However, industrial biochemical production often employs microbes with phenotypes that advantageous a target process, and may fail to directly transition outside the host which they developed. In particular, losses sensitivity dynamic range of sensing occur, limiting application...
Product inhibition is a frequent bottleneck in industrial enzymes, and testing mutations to alleviate product via traditional methods remains challenging as many variants need be tested against multiple substrate concentrations. Further, screening are conducted vitro, resulting enzyme may perform differently vivo the context of whole-cell metabolism regulation. In this study, we address these two problems by establishing high-throughput method an industrially relevant enzyme, chorismate...
Thermostabilization of an enzyme with complete retention catalytic efficiency was demonstrated on recombinant 3-dehydroshikimate dehydratase (DHSase or wtAsbF) from Bacillus thuringiensis serovar konkukian 97–27 (hereafter, B. 97–27). The wtAsbF is relatively unstable at 37 °C, in vitro (t1/237 = 15 min), the absence divalent metal. We adopted a structure-based design to identify stabilizing mutations and created combinatorial library based upon predicted specific locations surface. A...
Engineered microbes can be used for producing value-added chemicals from renewable feedstocks, relieving the dependency on nonrenewable resources such as petroleum. These often are composed of synthetic metabolic pathways; however, one major problem in establishing a pathway is challenge precisely controlling competing routes, some which could crucial fitness and survival. While traditional gene deletion and/or coarse overexpression approaches do not provide precise regulation,...
The authors present their Comprehensive Systems Collaboration (CSC) model, which facilitates collaboration among school, alcohol and drug treatment, other systems to coordinate services plan strategies help alcohol- drug-affected students. model provides a comprehensive approach the recovery process of such This article explains goals CSC role school social worker, practical material used assist professionals in establishing using this model.