A5078581374- 3D Printing in Biomedical Research
- Effects of Radiation Exposure
- Lung Cancer Treatments and Mutations
- Nanoparticles: synthesis and applications
- Pluripotent Stem Cells Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Ubiquitin and proteasome pathways
- Quantum Dots Synthesis And Properties
- Advancements in Transdermal Drug Delivery
- Tissue Engineering and Regenerative Medicine
- Biochemical and Structural Characterization
- Neuroscience and Neural Engineering
- Graphene and Nanomaterials Applications
- Fullerene Chemistry and Applications
- Radiation Therapy and Dosimetry
- Olfactory and Sensory Function Studies
- Chronic Lymphocytic Leukemia Research
- Computational Drug Discovery Methods
- Photoreceptor and optogenetics research
- Gold and Silver Nanoparticles Synthesis and Applications
- HER2/EGFR in Cancer Research
- Advanced biosensing and bioanalysis techniques
- DNA Repair Mechanisms
- Bacterial biofilms and quorum sensing
- Cancer Mechanisms and Therapy
Rutherford Appleton Laboratory
2020-2024
Teva Pharmaceuticals (United States)
2024
Immunocore (United Kingdom)
2023-2024
Los Alamos National Laboratory
2013-2023
Research Complex at Harwell
2020
Los Alamos Medical Center
2013-2018
Ninewells Hospital
2014-2017
University of Dundee
2014-2017
Institute of Cancer Research
2014
Center for Integrated Nanotechnologies
2012
Significance Effective treatment of skin-based bacterial biofilms has been identified as a serious and unmet medical need. Biofilm-protected bacteria account for ∼80% infections in humans are 50–1,000 times more resistant to antibiotics than their planktonic counterparts. Biofilms skin further protected by the outermost layer skin, stratum corneum, which serves natural barrier most therapeutics. Here, we present compelling evidence exploiting ionic liquids (ILs) an arsenal materials both...
The growing potential of quantum dots (QDs) in applications as diverse biomedicine and energy has provoked much dialogue about their conceivable impact on human health the environment at large. Consequently, there been an urgent need to understand interaction with biological systems. Parameters such size, composition, surface charge, functionalization can be modified ways either enhance biocompatibility or reduce deleterious effects. In current study, we simultaneously compared alone...
Abstract The Epidermal Growth Factor Receptor (EGFR) is frequently found to be mutated in non-small cell lung cancer. Oncogenic EGFR has been successfully targeted by tyrosine kinase inhibitors, but acquired drug resistance eventually overcomes the efficacy of these treatments. Attempts surmount this therapeutic challenge are hindered a poor understanding how and why cancer mutations specifically amplify ligand-independent auto-phosphorylation signals enhance survival amplification related...
Iyer, R. and Lehnert, B. E. Alpha-Particle-Induced Increases in the Radioresistance of Normal Human Bystander Cells. Radiat. Res. 157, 3–7 (2002).Numerous investigators have reported that direct exposure cells to a low dose ionizing radiation can induce condition enhanced radioresistance, i.e. "radioadaptive" response. In this report, we investigated hypothesis radioadaptive bystander effect may be induced unirradiated by transmissible factor(s) present supernatants exposed α particles....
Reactive oxygen species (ROS), which contribute to the energy landscapes in and around cells, play numerous roles maintaining normal cell homeostasis as components of signaling pathways. Excessively high levels ROS, on other hand, can lead pronounced DNA damage a variety cellular responses, including cycle arrests, senescence, apoptosis possibly cancer. Far less is known, however, about how supra-basal ROS that be generated response low doses ionizing radiation or chemicals environment may...
Quantum dots (QDs) are semiconductor nanocrystals exhibiting unique optical properties that can be exploited for many practical applications ranging from photovoltaics to biomedical imaging and drug delivery. A significant number of studies have alluded the cytotoxic potential these materials, implicating Cd-leaching as causal factor. Here, we investigated role heavy metals in biological responses CdSe-induced genotoxicity. Our results indicate that, while negatively charged QDs relatively...
A series of fluorescent unnatural amino acids (UAAs) bearing stilbene and <italic>meta</italic>-phenylenevinylene (<italic>m</italic>-PPV) backbone have been synthesized by palladium-catalyzed Heck couplings.
p68 (DDX5) acts both as an ATP-dependent RNA helicase and a transcriptional co-activator of several cancer-associated transcription factors, including the p53 tumor suppressor. is aberrantly expressed in high proportion cancers, but oncogenic drive for, or consequences of, these expression changes remain unclear. Here we show that elevated cohort human breast cancers associated significantly with levels protein kinase, Polo-like kinase-1 (PLK1). Patients expressing detectable PLK1 have poor...
A new compact and multifunctional hybrid semiconductor–metal nanostructure is elucidated demonstrated for real-time optical imaging, photothermal heating, <italic>in situ</italic> thermometry.
The aim of this review is to provide an overview physiologically relevant microengineered lung-on-a-chip (LoC) platforms for a variety different biomedical applications with emphasis on drug screening. First, brief outline lung anatomy and physiology presented followed by discussion the parenchyma its extracellular matrix. Next, we point out technical challenges in recapitulating complexity conventional static two-dimensional microenvironments need alternate platforms. importance scaling...
Abstract Background Human induced pluripotent stem cells (iPSC) have opened new avenues for regenerative medicine. Consequently, iPSC-derived motor neurons emerged as potentially viable therapies spinal cord injuries and neurodegenerative disorders including Amyotrophic Lateral Sclerosis. However, direct clinical application of iPSC bears in itself the risk tumorigenesis other unforeseeable genetic or epigenetic abnormalities. Results Employing RNA-seq technology, we identified characterized...
Exposure to silica dust can result in lung inflammation that may progress fibrosis for which there is no effective clinical treatment. The mechanisms involved the development of pulmonary silicosis have not been well defined; however, most current evidence implicates a central role alveolar macrophages this process. We previously demonstrated fibrotic agents, such as asbestos and silica, induce apoptosis human macrophages. goal study was identify molecular events silica-induced apoptotic...
When evaluating the toxicity of engineered nanomaterials (ENMS) it is important to use multiple bioassays based on different mechanisms action. In this regard we evaluated gene expression and common cytotoxicity measurements using as test materials, two selected nanoparticles with known differences in toxicity, 5 nm mercaptoundecanoic acid (MUA)-capped InP CdSe quantum dots (QDs). We tested effects these QDs at concentrations ranging from 0.5 160 µg/mL cultured normal human bronchial...
Initial experiments using differentially functionalized fullerenes, CD‐C 60 , hexa‐C and tris‐C suggested a properties dependent effect on cytotoxic proliferative responses in human skin keratinocytes. In the present study we investigated cytokine secretion profile of dermal epithelial cells exposed to fullerenes. Keratinocyte‐derived cytokines affect homing trafficking normal malignant epidermal immune as well nonimmune vivo. These are critical for regulating activation, proliferation,...
The accurate prediction of hepatotoxicity demands validated human in vitro models that can close the gap between preclinical animal studies and clinical trials. In this study we investigated response primary liver cells to toxic drug exposure a perfused microscale 3D bioreactor. cellularized bioreactors were treated with 5, 10, or 30 mM acetaminophen (APAP) used as reference substance. Lactate production significantly decreased upon treatment APAP (p < 0.05) ammonia release increased 10...
Using Neutron Reflectometry (NR), polyelectrolyte multilayer (PEM) films made by layer-by-layer (LbL) deposition of a strong polycation (polyethylene imine [PEI]) and polyanion (polystyrene sulfonate [PSS]) have been characterized. PEI terminated samples with total 5, 7, 9 layers were deposited on quartz substrate studied under three different environmental conditions (i.e., dry air, 100% relative humidity, bulk water). We able to model all the measurements at contrast using one simple,...
Surface adhered bacterial colonies or biofilms are an important problem in medical and food industries. Bacteria use a chemical language to monitor their quorum express virulence factors, which eventually help them colonization manifestation of infection. The LasR-LasI RhlR-RhlI quorum-sensing (QS) systems Pseudomonas aeruginosa control expression factors population density-dependent fashion. In this study we investigated the role synthetic analogs system P. strains (PAO-1; wild-type mutants...