A5011276566- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Plant Molecular Biology Research
- Electron and X-Ray Spectroscopy Techniques
- Plant Stress Responses and Tolerance
- Chronic Lymphocytic Leukemia Research
- PI3K/AKT/mTOR signaling in cancer
- Microfluidic and Capillary Electrophoresis Applications
- Cancer-related Molecular Pathways
- S100 Proteins and Annexins
- Ion-surface interactions and analysis
- Cell death mechanisms and regulation
- Advanced Glycation End Products research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Genetic diversity and population structure
- Marine Bivalve and Aquaculture Studies
- Evolution and Genetic Dynamics
- Coral and Marine Ecosystems Studies
- Force Microscopy Techniques and Applications
- Neonatal Respiratory Health Research
- Pregnancy-related medical research
- Cell Adhesion Molecules Research
- Leaf Properties and Growth Measurement
Uppsala University
2004-2025
Science for Life Laboratory
2018-2023
Karolinska Institutet
2004-2022
AstraZeneca (Sweden)
2020-2021
AstraZeneca (Singapore)
2020
Chalmers University of Technology
2015-2016
Karolinska University Hospital
1997-2012
Sanford Burnham Prebys Medical Discovery Institute
2008-2010
New York University
2010
Emory University
2010
Patients with inflammatory bowel diseases are at increased risk for colorectal cancer, but the molecular mechanisms linking inflammation and cancer not well defined. We earlier showed that carboxylated N-glycans expressed on receptor advanced glycation end products (RAGE) other glycoproteins mediate colitis through activation of nuclear factor kappa B (NF-κB). Because NF-κB signaling plays a critical role in pathogenesis colitis-associated (CAC), we reasoned glycans, RAGE its ligands might...
Members of the homeodomain leucine zipper (HDZip) family transcription factors are present in a wide range plants, from mosses to higher but not other eukaryotes. The HDZip genes act developmental processes, including vascular tissue and trichome development, several them have been suggested be involved mediation external signals regulate plant growth. Arabidopsis (Arabidopsis thaliana) genome contains 47 genes, which, based on sequence criteria, grouped into four different classes: I IV. In...
Chromosomal inversions can facilitate local adaptation in the presence of gene flow by suppressing recombination between well-adapted native haplotypes and poorly adapted migrant haplotypes. East African mountain populations honeybee Apis mellifera are highly divergent from neighbouring lowland at two extended regions genome, despite high similarity rest suggesting that these genomic harbour governing adaptation. Here, we utilize a new contiguous assembly genome to characterize regions....
Abstract Evidence is accumulating that gene flow commonly occurs between recently diverged species, despite the existence of barriers to in their genomes. However, we still know little about what regions genome become and how such form. Here, compare genetic differentiation across genomes bumblebee species living sympatry allopatry reveal potential impact during divergence uncover barrier loci. We first compared alpine Bombus sylvicola a previously unidentified sister Rocky Mountains,...
In order to identify regulatory genes involved in the development of an apoptosis-resistant phenotype patients with chemotherapy refractory B-cell chronic lymphocytic leukemia (B-CLL) expression apoptosis-regulating B-CLL cells was quantified using cDNA arrays and RT-PCR. Data were obtained from compared between 2 groups either nonprogressive, indolent, previously untreated disease leukemic sensitive vitro fludarabine-induced apoptosis, referred as (sB-CLL) or progressive, resistant...
Abstract The receptor for advanced glycation end products (RAGE) is a signaling protein of the immunoglobulin superfamily implicated in multiple pathologies. It binds diverse repertoire ligands, but structural basis interaction different ligands not well understood. We earlier showed that carboxylated glycans on V‐domain RAGE promote binding HMGB1 and S100A8/A9. Here we study role these intracellular mediated by another ligand, S100A12. S100A12 glycans, subpopulation enriched shows more than...
Tomography using a focused ion beam (FIB) combined with scanning electron microscope (SEM) is well-established for wide range of conducting materials. However, performing FIB–SEM tomography on ion- and electron-beam-sensitive materials as well poorly soft remains challenging. Some common challenges include cross-sectioning artifacts, shadowing effects, charging. Fully dense provide planar cross section, whereas pores also expose subsurface areas the cross-section surface. The image intensity...
Meiotic recombination is a fundamental evolutionary process that facilitates adaptation and the removal of deleterious genetic variation. Social Hymenoptera exhibit some highest rates among metazoans, whereas high have not been found nonsocial species from this insect order. It unknown whether elevated are ubiquitous feature all social insects. In many metazoan taxa, mainly restricted to hotspots few kilobases in length. However, little known about prevalence genomes. Here we infer rate its...
SUMMARY Mitochondrial abnormalities drive subcutaneous white adipose tissue dysfunction in obesity, leading to metabolic complications. A key challenge obesity research is the lack of vitro models study human adipocyte mitochondria. Here, we establish a spheroid model characterize mitochondrial metabolism under obesity-relevant conditions and drug exposure. Human preadipocyte spheroids were differentiated ultra-low attachment plates for 3 weeks media free thiazolidinediones. The showed...
B-cell chronic lymphocytic leukaemia (B-CLL) is characterised by the progressive accumulation of monoclonal CD5(+) B cells. In a previous study, we have analysed expression profile apoptosis-regulating genes using cDNA-based microarray and found overexpression antiapoptotic bcl-2 family member, bfl-1, in B-CLL cells with an apoptosis-resistant phenotype. this bfl-1 mRNA levels been determined competitive PCR extended population patients to characterise its role disease progression...
The eastern honey bee (Apis cerana) is of central importance for agriculture in Asia. It has adapted to a wide variety environmental conditions across its native range southern and Asia, which includes high-altitude regions. bees inhabiting mountains differ morphologically from neighbouring lowland populations may also exhibit differences physiology behaviour. We compared the genomes 60 collected high low altitudes Yunnan Gansu provinces, China, infer their evolutionary history identify...
Summary Combined focused ion beam and scanning electron microscope (FIB‐SEM) tomography is a well‐established technique for high resolution imaging reconstruction of the microstructure wide range materials. Segmentation FIB‐SEM data complicated due to number factors; most prominent that porous materials, image slices contain information not only from planar cross‐section material but also underlying, exposed subsurface pores. In this work, we develop segmentation method ethyl cellulose films...
Genomic stability is one of the crucial prognostic factors for patients with endometrioid endometrial cancer (EEC). The impact genomic on tumour tissue proteome EEC not yet well established.Tissue lysates EEC, squamous cervical (SCC), normal endometrium and epithelium were subjected to two-dimensional (2D) gel electrophoresis identification proteins by MALDI TOF MS. Expression selected was analysed in independent samples immunohistochemistry.Diploid aneuploid genomically unstable displayed...
Abstract Meiotic recombination shuffles genetic variation and promotes correct segregation of chromosomes. Rates vary on several scales, both within genomes between individuals, this is affected by environmental factors. Social insects have extremely high rates recombination, although the evolutionary causes are not known. Here, we estimate crossovers gene conversions in 22 colonies honeybee, Apis mellifera, 9 bumblebee, Bombus terrestris, using direct sequencing 299 haploid drone offspring....
<h3>OBJECTIVE</h3> To assess the frequency of valvar complications caused by left sided radiofrequency catheter ablation using retrograde aortic technique. <h3>METHODS</h3> 179 patients (118 male) with a mean (SD) age 43 (17) years underwent 216 procedures at one centre. The target was an accessory atrioventricular pathway in 144 patients, junction 29 and ventricular tachycardia six patients. In 25 structural heart disease identified before procedure (ischaemic 10, cardiomyopathy nine,...
Abstract Krill are vital as food for many marine animals but also impacted by global warming. To learn how they and other zooplankton may adapt to a warmer world we studied local adaptation in the widespread Northern krill ( Meganyctiphanes norvegica ). We assemble characterize its large genome compare genome-scale variation among 74 specimens from colder Atlantic Ocean Mediterranean Sea. The 19 Gb likely evolved through proliferation of retrotransposons, now targeted inactivation extensive...
A range of different genetic architectures underpin local adaptation in nature. Honey bees (Apis mellifera) the Eastern African Mountains harbor high frequencies two chromosomal inversions that likely govern to this high-elevation habitat. In Americas, honey are hybrids European and ancestries latitudinal variation climate correlates with proportion these across genome. It is unknown which, if either, forms governs living at elevations Americas. Here, we performed whole-genome sequencing 29...
Over the last six decades, populations of bumblebees Bombus sylvicola and balteatus in Colorado have experienced decreases tongue length, a trait important for plant-pollinator mutualisms. It has been hypothesized that this observation reflects selection resulting from shifts floral composition under climate change. Here we used morphometrics population genomics to determine whether morphological change is ongoing, investigate genetic basis variation, analyse structure these populations. We...
Abstract Populations of many bumblebee species are declining, with distributions shifting northwards to track suitable climates. Climate change is considered a major contributing factor. Arctic particularly vulnerable as they cannot shift further north, making assessment their population viability important. Analysis levels whole‐genome variation powerful way analyse declines and fragmentation. Here, we use genome sequencing genetic in seven from the Scandinavian mountains, including two...