- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Wheat and Barley Genetics and Pathology
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant pathogens and resistance mechanisms
- Animal Genetics and Reproduction
- Agricultural pest management studies
- Plant Reproductive Biology
- Cocoa and Sweet Potato Agronomy
- GABA and Rice Research
- Neuroscience of respiration and sleep
- Proteins in Food Systems
- Agronomic Practices and Intercropping Systems
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Resistance
- Enzyme-mediated dye degradation
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogenic Bacteria Studies
- Prenatal Substance Exposure Effects
- Neonatal Health and Biochemistry
- Genetically Modified Organisms Research
- Genetics and Plant Breeding
Syngenta (United States)
2024
The University of Texas Health Science Center at Houston
1997
Kansas State University
1995
University of Wisconsin–Madison
1987-1991
Bovine trypsin (EC 3.4.21.4) is an enzyme that widely used for commercial purposes to digest or process other proteins, including some therapeutic proteins. The biopharmaceutical industry trying eliminate animal‐derived proteins from manufacturing processes due the possible contamination of these products by human pathogens. Recombinant has been produced in a number systems, cell culture, bacteria and yeast. To date, expression systems have not on scale sufficient fulfill need manufacturers...
Summary Expression of industrial enzymes in transgenic plants offers an alternative system to fungal fermentation for large‐scale production. Very high levels expression are required make the cost‐effective. We tested several parameters determine best method achieving a laccase gene. Transgenic maize were generated using Agrobacterium ‐mediated system. The molecular that induced highest embryo‐preferred globulin 1 promoter and targeting protein cell wall. Two independent events yielded...
The availability of foods low in sugar content yet high flavour is critically important to millions individuals conscious carbohydrate intake for diabetic or dietetic purposes. Brazzein a sweet protein occurring naturally tropical plant that impractical produce economically on large scale, thus limiting its food products. We report here the use maize expression system production this protein. High brazzein was obtained, with accumulation up 4% total soluble seed. Purified corn possessed...
Manganese peroxidase (MnP) has been implicated in lignin degradation and thus potential applications pulp paper bleaching, enzymatic remediation the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, particular their for large-scale industrial enzyme production. Several plant vectors were created to evaluate accumulation of MnP from wood-rot fungus Phanerochaete chrysosporium maize seed. We showed cell wall...
The Rp8 rust resistance locus of maize is unique in that only Rp8-A/Rp8-B heterozygotes provide and homozygotes are susceptible. A third allele, Rp8-C, provides no or heterozygous combinations. maps to the long arm chromosome 6, near restriction fragment length polymorphism marker umc21.
A targeted mapping strategy using representational difference analysis (RDA) was employed to isolate new restriction fragment length polymorphism probes for the long arm of chromosome 6 in rye (6RL), which carries a gene resistance Hessian fly larvae. Fragments from 6RL were specifically isolated 'Chinese Spring' (CS) wheat – ditelosomic addition line (CSDT6RL) as tester, and CS (or) CS4R driver genomic subtraction. Three RDA experiments performed BamHI amplicons, two successful producing...
The objective of this study was to determine the rate recent cocaine use among a metropolitan population predominantly Hispanic and African-American women with preterm premature rupture membranes (PROM) ascertain impact on latency period between delivery. Urine toxicology screens were prospectively obtained 147 PROM. urine screen did not influence management decisions. All expectantly managed without tocolytics until 37 weeks' gestation unless they developed clinical chorioamnionitis, or...
Abstract In an effort to develop a high-yielding, determinate plant type with multiple lateral branches, crosses were made between two Cucumis sativus var. lines (‘Spacemaster’ and NCSU M27) C. hardwickii (R.) Alef. line, LJ 90430. Individual data from six generations (P 1 , P 2 F BC ) subjected analysis of generation means determine the importance gene effects for number nodes, primary average internode length, main stem fruit per at maturity. A simple additive-dominance model adequately...