- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Plant Pathogens and Resistance
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Plant Genetic and Mutation Studies
- Plant Pathogens and Fungal Diseases
- Weed Control and Herbicide Applications
- Genomics and Phylogenetic Studies
- Bioenergy crop production and management
- Plant Taxonomy and Phylogenetics
- Plant-Microbe Interactions and Immunity
- Mycotoxins in Agriculture and Food
- Plant Reproductive Biology
- Crop Yield and Soil Fertility
- Genetic diversity and population structure
- Yeasts and Rust Fungi Studies
- Biofuel production and bioconversion
- Agriculture, Plant Science, Crop Management
- Plant biochemistry and biosynthesis
- CRISPR and Genetic Engineering
- Plant pathogens and resistance mechanisms
Kansas State University
2014-2023
Klinikum Magdeburg
2016
Planta
2006
Plant (United States)
2006
North Carolina State University
1994
University of Saskatchewan
1994
Technical University of Munich
1987-1992
Weihenstephan-Triesdorf University of Applied Sciences
1992
Bavarian State Research Center for Agriculture
1989
Freie Universität Berlin
1976-1989
An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum) genome has been produced by sequencing isolated chromosome arms. We have annotated 124,201 gene loci distributed nearly evenly across homeologous chromosomes and subgenomes. Comparative analysis subgenomes extant diploid tetraploid relatives showed that high similarity structural conservation are retained, with limited loss, after polyploidization. However, genomes there was evidence dynamic gain,...
The allohexaploid bread wheat genome consists of three closely related subgenomes (A, B, and D), but a clear understanding their phylogenetic history has been lacking. We used assemblies five diploid relatives to analyze genome-wide samples gene trees, as well estimate evolutionary relatedness divergence times. show that the A B genomes diverged from common ancestor ~7 million years ago these gave rise D through homoploid hybrid speciation 1 2 later. Our findings imply present-day is product...
A standard karyotype based on N-banding, C-banding, and modified C-banding has been constructed for Triticum aestivum L. 'Chinese Spring'. An idiogram a nomenclature system have developed the description of individual bands. Nomenclatural rules proposed chromosomal structural aberrations polymorphic bands in other wheat cultivars. As rule each short arm (S) long (L) consists series dark (C-bands) light (mainly euchromatic) by definition there are no interbands. In some cases, subdivided into...
Allohexaploid bread wheat ( Triticum aestivum L.) provides approximately 20% of calories consumed by humans. Lack genome sequence for the three homeologous and highly similar genomes (A, B, D) has impeded expression analysis grain transcriptome. We used previously unknown information to analyze cell type–specific genes in developing identified distinct co-expression clusters reflecting spatiotemporal progression during endosperm development. observed no global but type– stage-dependent...
Significance Glyphosate is a nonselective herbicide used around the globe for weed control in glyphosate-resistant (GR) and noncrop situations. The extensive exclusive use of glyphosate has led to evolution resistance many crop weeds. molecular target glyphosate, 5-enolpyruvlyshikimate-3-phosphate synthase ( EPSPS ) gene, confers upon amplification was first documented GR Amaranthus palmeri . We now report that amplified copies A. are present form extrachromosomal circular DNA molecules...
Summary We investigated the composition and basis of genome expansion in core Triticeae using Aegilops tauschii , D‐genome donor bread wheat. sequenced an unfiltered genomic shotgun (trs) a methylation–filtration (tmf) library A. analyzed wheat expressed sequence tags (ESTs) to estimate expression genes transposable elements (TEs). The sampled sequences consisted 91.6% repetitive elements, 2.5% known genes, 5.9% low‐copy unknown function. TEs constituted 68.2% compared with 50% maize 14%...
Recent rapid evolution and spread of resistance to the most extensively used herbicide, glyphosate, is a major threat global crop production. Genetic mechanisms by which weeds evolve herbicides largely determine level rate resistance. In previous study, we determined that glyphosate in Kochia scoparia due amplification 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) gene, enzyme target glyphosate. Here, investigated genomic organization amplified EPSPS copies using fluorescence situ...
A cytogenetic map of wheat was constructed using FISH with cDNA probes. markers detected homoeology and chromosomal rearrangements wild relatives, an important source genes for improvement. To transfer agronomically from relatives to bread (Triticum aestivum L., 2n = 6 x 42, AABBDD) by induced homoeologous recombination, it is know the relationships species involved. Fluorescence in situ hybridization (FISH) can be used study chromosome structure. The genomes allohexaploid other Triticeae...
Abstract Key message The first cytological characterization of the 2N v S segment in hexaploid wheat; complete de novo assembly and annotation segment; frequency is increasing associated with higher yield. Aegilops ventricosa translocation has been utilized breeding disease-resistant wheat crops since early 1990s. This known to possess several important resistance genes against multiple diseases including root knot nematode, stripe rust, leaf rust stem rust. More recently, this blast, an...
Genome differentiation in 12 diploid Aegilops species was analyzed using situ hybridization with the highly repetitive DNA sequences pSc119 and pAs1 C-banding. Chromosomes of all these hybridized probe; however, level labeling patterns differed among genomes. Only four (Ae. squarrosa, Ae. comosa, heldreichii, uniaristata) showed distinct pAs1. The were species-specific chromosome-specific. Differences (ISH) patterns, also observed by C-banding, exist between karyotypes comosa suggesting that...
The distribution of the 5S and 18S-5.8S-26S (18S-26S) ribosomal RNA (rRNA) gene families on chromosomes all diploid Aegilops species was studied by in situ hybridization with pTa71 (18S-26S rDNA) pTa794 (5S DNA clones. One major 18S-26S rDNA locus found nucleolus organizer region (NOR) each tauschii uniaristata two loci were detected remaining species. In addition to NORs, from one nine minor observed; their numbers chromosomal locations species-specific. Some polymorphic, whereas others...