A5075003126- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Nanoplatforms for cancer theranostics
- Extracellular vesicles in disease
- Carbon and Quantum Dots Applications
- Biosensors and Analytical Detection
- Photoreceptor and optogenetics research
- Advanced Nanomaterials in Catalysis
Tianjin University
2021-2025
Shanghai Cancer Institute
2024
Renji Hospital
2024
Shanghai Jiao Tong University
2024
Fudan University
2024
Constructing artificial dynamic architectures inside cells to rationally interfere with organelles is emerging as an efficient strategy regulate the behaviors and fate of cells, thus providing new routes for therapeutics. Herein, we develop intracellular K+-mediating assembly DNA tetrahedrons which realizes mitochondrial interference consequent regulation on energy metabolism living cells. In designer tetrahedron, one vertex was modified triphenylphosphine (TPP) targeting, other three...
CRISPR/Cas9 is emerging as a platform for gene therapeutics, and the treatment efficiency expected to be enhanced by combination with other therapeutic agents. Herein, we report proton-activatable DNA-based nanosystem that enables co-delivery of Cas9/sgRNA DNAzyme combined therapy cancer. Ultra-long ssDNA chains, which contained recognition sequences sgRNA in Cas9/sgRNA, sequence HhaI enzyme cleavage site, were synthesized scaffold nanosystem. The cofactor Mn2+ was used compress DNA chains...
Photodynamic therapy (PDT) depends on the light-irradiated exciting of photosensitizer (PS) to generate reactive oxygen species (ROS), which faces challenges and limitations in hypoxia antioxidant response cancer cells, limited tissue-penetration light. Herein, a multifunctional DNA/upconversion nanoparticles (UCNPs) complex is developed enables controlled co-delivery CRISPR-Cas9, hemin, protoporphyrin (PP) for synergistic PDT. An ultralong single-stranded DNA (ssDNA) prepared via rolling...
Abstract Coupling materials chemistry systems to biological processes is a promising way rationally modulate lysosomal functions. A proton‐driven dynamic assembly of DNA nanoframework inside cells coupled with the lysosome‐mediated endocytosis pathways/lysosomal maturation, gives rational modulation functions, which we term “lysosome interference”. Through endocytosis, acid‐responsive semi‐i‐motif enters lysosome and assembles into an aggregate in process triggered by acidity. The suitable...
The precise control of the artificially induced reactions inside living cells is emerging as an effective strategy for regulation cell functions. Nevertheless, manipulation assembly exogenous molecules into artificial architectures in response to intracellular-specific signals remains a grand challenge. Herein, we achieve self-assembly deoxyribonucleic acid (DNA) network cancer cells, specifically responding telomerase, and realize mitochondrial interference consequent cellular behaviors....
CRISPR-Cas9 has been explored as a therapeutic agent for down-regulating target genes; the controlled delivery of Cas9 ribonucleoprotein (RNP) is essential efficacy and remains challenge. Here, we report cascade dynamic assembly/disassembly DNA nanoframework (NF) that enables RNP. NF was prepared with acrylamide-modified initiated hybridization chain reaction (HCR). Through an HCR, single-guide RNA incorporated to NF; simultaneously, internal space expanded, facilitating loading protein....
Abstract CRISPR‐Cas9 has demonstrated potential in inhibiting tumor growth by downregulating target gene expression, and the efficiency is expected to be enhanced with other nucleic acid drugs. Herein, a dual‐enzyme–responsive DNA‐based nanoframework (NF) that enables controlled co‐delivery of Cas9 ribonucleoprotein (RNP) antisense oligodeoxynucleotide (ASO) for synergistic therapy reported. NF synthesized acrylamide DNA (acDNA) as cross‐linker loading site. A ternary complex containing...
ConspectusIn recent years, the controlled assembly/disassembly of exogenous chemical components inside cells has become an emerging approach to regulating cell functions. However, construction dynamic material chemistry systems in living always remains highly challenging due complicated intracellular microenvironment. Nucleic acid is a category biological that can achieve efficient molecular assembly via specific base-pairing and perform functions Deoxyribonucleic (DNA) molecules exhibit...
Theranostic platforms that integrate diagnostic and therapeutic functionalities offer promising strategies for precision medicine, particularly in the treatment of major diseases. However, development capable achieving spatially controlled detection therapy at lesion site remains a significant challenge. Herein, we present dual‐stimuli‐responsive DNA nanoframework achieve co‐delivery molecular beacon (MB) Cas9 ribonucleoprotein (RNP), enabling simultaneous specific optical efficient gene...
Theranostic platforms that integrate diagnostic and therapeutic functionalities offer promising strategies for precision medicine, particularly in the treatment of major diseases. However, development capable achieving spatially controlled detection therapy at lesion site remains a significant challenge. Herein, we present dual‐stimuli‐responsive DNA nanoframework achieve co‐delivery molecular beacon (MB) Cas9 ribonucleoprotein (RNP), enabling simultaneous specific optical efficient gene...
Abstract Exploring appropriate precursors has been proposed to be a promising strategy for the creation of artificial enzymes that are emerging as alternatives natural enzymes. Herein, inspired by catalytic activities ribose nucleic acid, using ribonucleosides including adenosine, guanosine, cytidine, and uridine, respectively, four carbonic aggregates, namely, carbon dots (A‐CDs, G‐CDs, C‐CDs, U‐CDs) mimic synthesized. All CDs show planar graphene‐like structure thus can intercalatively...
Abstract CRISPR/Cas9 system is promising for the reversal of tumor immunosuppression in immunotherapy, but controlled delivery remains challenging. Herein, study reported a smart DNA nanoassembly containing multivalent aptamers, realizing Cas9/sgRNA ribonucleoprotein (RNP) enhanced cancer immunotherapy. A single‐stranded complementary to sgRNA RNP can initiate cascade‐clamped hybridization chain reaction (C‐HCR) wrap up nanoassembly. After selective internalization by cells, released...
Abstract CRISPR/Cas9 is emerging as a platform for gene therapeutics, and the treatment efficiency expected to be enhanced by combination with other therapeutic agents. Herein, we report proton‐activatable DNA‐based nanosystem that enables co‐delivery of Cas9/sgRNA DNAzyme combined therapy cancer. Ultra‐long ssDNA chains, which contained recognition sequences sgRNA in Cas9/sgRNA, sequence HhaI enzyme cleavage site, were synthesized scaffold nanosystem. The cofactor Mn 2+ was used compress...
Abstract Coupling materials chemistry systems to biological processes is a promising way rationally modulate lysosomal functions. A proton‐driven dynamic assembly of DNA nanoframework inside cells coupled with the lysosome‐mediated endocytosis pathways/lysosomal maturation, gives rational modulation functions, which we term “lysosome interference”. Through endocytosis, acid‐responsive semi‐i‐motif enters lysosome and assembles into an aggregate in process triggered by acidity. The suitable...