Mechanical properties of cells and extracellular matrix (ECM) play important roles in many biological processes including stem cell differentiation, tumor formation, wound healing. Changes stiffness ECM are often signs changes physiology or diseases tissues. Hence, is an index to evaluate the status cultures. Among multitude methods applied measure tissues, micro-indentation using Atomic Force Microscope (AFM) provides a way reliably living cells. This method has been widely characterize...

Abstract Enhanced oil recovery (EOR) plays a significant role in improving production. Tertiary EOR, including surfactant flooding, can potentially mobilize residual after water flooding. Prior to the field deployment, performance must be evaluated using site-specific crude at reservoir conditions. Core flood experiments are common practice evaluate surfactants for displacement efficiency core samples. experiments, however, expensive and time-consuming do not allow pore scale observations of...

Abstract Orthogonal functionalization of 2D materials by selective assembly at interfaces provides opportunities to create new with transformative properties. Challenges remain in realizing controllable, scalable surface‐selective, and orthogonal functionalization. Herein, dynamic covalent is reported that directs the graphene surfaces liquid–liquid interfaces. This process allows facile addition segregation chemical functionalities impart Janus characteristics graphenes. Specifically,...

Abstract Glioblastoma multiforme (GBM) is an aggressive, Grade IV astrocytoma with a poor survival rate, primarily due to the GBM tumor cells migrating away from primary site along nanotopography of white matter tracts and blood vessels. It unclear whether this influences biomechanical properties (i.e. cytoskeletal stiffness) cells. Although have innate propensity migrate, we believe capability enhanced influence on cells’ properties. In study, used aligned nanofiber film that mimics in...

Current red-blood-cell cryopreservation methods utilize bulk volumes, causing cryo-injury of cells, which results in irreversible disruption cell morphology, mechanics, and function. An innovative approach to preserve human function following vitrification nanoliter volumes is developed using a novel cryo-ink integrated with bioprinting approach.

Mechanical properties of cells and extracellular matrix (ECM) play important roles in many biological processes including stem cell differentiation, tumor formation, wound healing. Changes stiffness ECM are often signs changes physiology or diseases tissues. Hence, is an index to evaluate the status cultures. Among multitude methods applied measure tissues, micro-indentation using Atomic Force Microscope (AFM) provides a way reliably living cells. This method has been widely characterize...

Cancer metastasis is a major cause of cancer-induced deaths in patients. Mimicking nanostructures an extracellular matrix surrounding cancer cells can provide useful clues for metastasis. This paper compares the morphology, proliferation, spreading, and stiffness highly aggressive glioblastoma multiforme normal fibroblast seeded on variety ordered polymeric (nanopillars nanochannels). Both cell lines survive proliferate nanostructured surface show more similarity surfaces than flat surfaces....

Abstract We are developing an integrated, real-time system for deploying Advanced Tracers cost-effectively in a ubiquitous and potentially long-term way. This campaign is the sake of increasing oil recovery factor large waterflooded reservoirs through improved optimization water injection production. paper explains key features this novel reports main results from ongoing field test our second-generation tracer material detection methodology. Existing inter-well tracers require elaborate...

Chemical tracing within oil reservoirs enables the determination of fluid allocation and interwell flow characteristics for improving injection sweep efficiency reservoir management, thus novel instrument-readable, independently distinguishable tracers with ultralow sensitivity detectability are highly desired. We present a series dipicolinic acid (DPA)-based molecules as new class water surveillance applications, demonstrating design synthesis three DPA-based confirmed characterized via NMR...

Optical tweezers play an important role in biological applications. However, it is difficult for traditional optical based on objective lenses to work a three-dimensional (3D) solid far away from the substrate. In this work, we develop fiber trapping system, namely inclined dual tweezers, that can simultaneously apply and measure forces both water 3D polyacrylamide gel matrix. addition, demonstrate situ, non-invasive characterization of local mechanical properties by measurements embedded...

The immediate physical and chemical surroundings of cells provide important biochemical cues for their behavior. Designing tailoring biomaterials controlled cell signaling extracellular matrix (ECM) can be difficult due to the complexity cell-surface relationship. To address this issue, our research has led development a polydimethylsiloxane (PDMS) scaffold with defined microtopography chemistry surface driven ECM assembly. When human fibroblasts were cultured on microtextured PDMS 2-6 µm...

Abstract We are developing new classes of barcoded advanced tracers, which, compared to present commercial offerings, can be optically detected in an automated fashion. The eventual goal for the tracers is deploy cost-effective, ubiquitous, long-term, and full-field tracer tests supporting large-scale waterflooding optimization improved oil recovery. In this paper, we compare model predictions breakthrough data from two field a pilot during water alternating gas (WAG) cycles, where have...

Chemical tracers are indispensable tools for enhancing reservoir characterization and optimizing production processes in the oil gas industry. Particularly, interwell water provide key data efficient flood management improvement of rates. However, analysis these within fluids is challenging, requiring laborious separation extraction steps that often rely on complex instruments skilled operators. Real-time especially problematic remote areas with limited access to well-equipped laboratories....

Abstract Objectives/Scope A novel barcoded advanced tracers system has been developed and field-validated in a hydrocarbon reservoir. With detection scheme that supports automation, this cost-effective is designed to enable potentially ubiquitous long-term full-field injections the interest of using high fidelity tracer data increase oil recovery factor through improved optimization water injection production. Methods, Procedures, Process Our Advanced Tracers uses real-time chromatographic...

Abstract The development of automatable high sensitivity analytical methods for tracer detection has been one the most central challenges to realize ubiquitous full-field deployment study reservoirs with many cross-communicating injector and producer wells. Herein we report a analysis approach, inspired by strategies commonly utilized in biotechnology industry, that directly addresses key limitations process throughput, automation potential state-of-the-art technologies. A two-dimensional...

Summary The use of tracer technology to illuminate reservoir characteristics such as well connectivity, volumetric sweep efficiency, and geological heterogeneity for the purpose improving history-matching fidelity enriching production optimization algorithms has gained momentum over last decade. Herein, we report stringent laboratory qualification a novel class fluorescent molecules, optically detectable down ultratrace levels (<ppb) in produced water, competent crosswell water...

Abstract Nanofluid flooding is an emerging enhanced oil recovery (EOR) technique whereby injection fluids containing nanoparticles or nanocomposites are utilized for the displacement injectivity improvement in reservoirs. However, current nanofluids with conventional have relatively low efficiency EOR at simulated reservoir conditions, especially concentrations of nanoparticles. This research reported a new cost-effective method scale-up synthesis Janus graphene nanosheets and demonstrated...

Tracers, or ″markers″, have extensive applications in monitoring the subsurface and environment. Oil gas fields apply a series of molecular tracers for interwell to support reservoir management. In near wellbore applications, help identify zonal flow allocation. Continuous using different is particularly appealing mud logging operations that require precise real-time assessment formation. Mineral particles (cuttings), generated during drilling, can provide valuable petrophysical lithological...

Abstract Mud logging is a technology with great potential for evaluating formations, but subpar accuracy in depth assignment cuttings. We have demonstrated the feasibility of pumping styrenic nanoparticles (NanoTags) into drilling mud and labelling drill cuttings improved assessment.1 Over years, we developed several generations new tags2-5 that minimum accumulation require fewer steps sample preparation before detection, thus promising solution in-field tag detection. Here report evolution...

Summary Drill cuttings logging (mud logging) is a technology with great potential to deliver formation evaluation and completion efficiency. However, the conventional mud determines cutting sample depth using lag time of cutting’s return trip, which results in uncertainties ±20 ft or more. We previously proposed tag at bit face penetrating, impregnating polymeric NanoTags determine cuttings’ NanoTag’s downward trip time, could reduce ±1–2 ft. The first field test generation was completed...

Abstract This research presents a novel and sustainable method to produce Janus carbon nanofluids from waste plastics. These show promise as highly effective agents for Enhanced Oil Recovery (EOR) under reservoir conditions. A cost-effective method, combining pyrolysis, chemical functionalization, pulverization, was developed synthesize nanoparticles (JC-NPs) plastics on an industrial scale. The properties of these nanostructures were confirmed using techniques like scanning electron...

We developed an inclined dual fiber optical tweezers (DFOTs) for simultaneous force application and measurements in a 3D hydrogel matrix. The DFOTs provide potential solution cell mechanics study three-dimensional