A5017032702- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Seed Germination and Physiology
- Analytical Chemistry and Chromatography
- Ion-surface interactions and analysis
- Identification and Quantification in Food
- Medical Imaging Techniques and Applications
- Plant Genetic and Mutation Studies
- Nuclear Physics and Applications
- Genetically Modified Organisms Research
- Plant Pathogens and Fungal Diseases
- Microbial Natural Products and Biosynthesis
- Luminescence and Fluorescent Materials
- Lipid metabolism and biosynthesis
- Soybean genetics and cultivation
- Molecular Biology Techniques and Applications
- Plant tissue culture and regeneration
- Biocrusts and Microbial Ecology
- Fungal Biology and Applications
- Viral Infectious Diseases and Gene Expression in Insects
State Ethnic Affairs Commission
2022-2024
Minzu University of China
2022-2024
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which is a label-free technique, determines the spatial distribution and relative abundance of versatile endogenous metabolites in tissues. Meanwhile, matrix selection generally regarded as pivotal step MALDI tissue imaging. This study presents first report novel matrix, 2-hydroxy-5-nitro-3-(trifluoromethyl)pyridine (HNTP), for
Herbicide-resistant soybeans are among the most widely planted transgenic crops. The in situ evaluation of spatial lipidomics and non-transgenic is important for directly assessing unintended effects exogenous gene introduction. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)-based non-targeted analytical strategies were used first time detection endogenous lipid distributions (EPSPS PAT genes) herbicide-resistant soybean (Glycine max Merrill)...
A novel metabolomics analysis technique, termed matrix-assisted laser desorption/ionization mass spectrometry imaging-based plant tissue microarray (MALDI-MSI-PTMA), was successfully developed for high-throughput metabolite detection and imaging from tissues. This technique completely overcomes the disadvantage that metabolites cannot be accessible on an intact due to limitations of special structures cells (e.g. epicuticular wax, cuticle cell wall) through homogenization tissues,...
Amino acids (AAs), which are low-molecular-weight (low-MW) metabolites, serve as essential building blocks not only for protein synthesis but also maintaining the nitrogen balance in living systems. In situ detection and imaging of AAs crucial understanding more complex biological processes. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MSI) is a label-free spectrometric technique that enables simultaneous spatial distribution relative abundance different...
In this study, polyacrylamide gel (PAAG) was successfully used as a new embedding medium to provide the more effective maintenance of biological tissues during sectioning process, enhancing tissue imaging metabolites via matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MSI). Herein, PAAG, agarose, gelatin, optimal cutting temperature compound (OCT), and ice media were embed rat liver Atlantic salmon (Salmo salar) eyeball samples. These embedded then sectioned into thin...
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a powerful approach that has been widely used for in situ detection of various endogenous compounds tissues. However, there are still challenges with analysis proteins using MALDI-MSI due to the ion suppression effects small molecules tissue sections. Therefore, tissue-washing steps crucial protein MALDI remove these interfering molecules. Here, we successfully developed new method named...
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Plant samples with irregular morphology are challenging for longitudinal tissue sectioning. This has restricted the ability to gain insight into some plants using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Herein, we develop a novel technique termed electromagnetic field-assisted frozen planarization (EMFAFTP). involves pair of adjustable electromagnets on both sides plant tissue. Under an optimized field strength, nondestructive and regularization is...
<p indent="0mm">Recently, mass spectrometry imaging (MSI) has drawn more and attention due to its continuous development improvement as well vital role in the study of biological science. Many MSI-based strategies have been widely applied <italic>in situ</italic> qualitative quantitative detection endogenous/exogenous molecules tissues. Among them, matrix assisted laser desorption/ionization MSI (MALDI-MSI) desorption electrospray ionization (DESI-MSI) are generally regarded most commonly...