A5082534517- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Ion-surface interactions and analysis
- Molecular Biology Techniques and Applications
- Computational Drug Discovery Methods
- Seed Germination and Physiology
- Genetically Modified Organisms Research
- Soybean genetics and cultivation
- Analytical Chemistry and Sensors
- Luminescence and Fluorescent Materials
- Biosensors and Analytical Detection
- Natural Antidiabetic Agents Studies
- dental development and anomalies
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Dental Health and Care Utilization
- Phytochemistry and Biological Activities
- Biocrusts and Microbial Ecology
- Estrogen and related hormone effects
- Urticaria and Related Conditions
- Oral microbiology and periodontitis research
- Spectroscopy and Chemometric Analyses
- Plant tissue culture and regeneration
National Clinical Research
2025
Minzu University of China
2018-2024
State Ethnic Affairs Commission
2021-2024
Tongji University
2016
Nanjing University
2009
Low-molecular-weight (low-MW) compounds have many essential functions in biological processes, and the molecular imaging of as low-MW possible is critical for understanding complex processes. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MSI) an emerging molecular-imaging technology that enables determination spatial distributions relative abundances diverse endogenous tissues. New matrices suitable by MALDI-MSI are important technological advancement tissue imaging....
To our knowledge, this was the first study in which caffeic acid (CA) successfully evaluated as a matrix to enhance situ detection and imaging of endogenous proteins three biological tissue sections (i.e., rat brain Capparis masaikai germinating soybean seeds) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MSI). Our results show several properties CA, including strong ultraviolet absorption, super-wide MS range close 200,000 Da, micrometer-sized crystals, uniform...
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which is a label-free technique, determines the spatial distribution and relative abundance of versatile endogenous metabolites in tissues. Meanwhile, matrix selection generally regarded as pivotal step MALDI tissue imaging. This study presents first report novel matrix, 2-hydroxy-5-nitro-3-(trifluoromethyl)pyridine (HNTP), for
Diabetes and periodontitis, as widespread chronic diseases, often exacerbate each other's conditions. Nonsurgical periodontal treatments can improve the oral systemic health in these patients, with salivary proteins offering potential insights into disease mechanisms treatment effectiveness. However, there is a lack of comprehensive data on proteomics this context. By assigning patients diabetes-associated periodontitis to test group (supragingival scaling, subgingival scraping root planing)...
DCH (2,3-dicyanohydroquinone), a fluorescent dye, was reported as novel and powerful (+) MALDI matrix for lipid tissue imaging.
Herbicide-resistant soybeans are among the most widely planted transgenic crops. The in situ evaluation of spatial lipidomics and non-transgenic is important for directly assessing unintended effects exogenous gene introduction. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)-based non-targeted analytical strategies were used first time detection endogenous lipid distributions (EPSPS PAT genes) herbicide-resistant soybean (Glycine max Merrill)...
A novel metabolomics analysis technique, termed matrix-assisted laser desorption/ionization mass spectrometry imaging-based plant tissue microarray (MALDI-MSI-PTMA), was successfully developed for high-throughput metabolite detection and imaging from tissues. This technique completely overcomes the disadvantage that metabolites cannot be accessible on an intact due to limitations of special structures cells (e.g. epicuticular wax, cuticle cell wall) through homogenization tissues,...
Summary Phytohormones possess unique chemical structures, and their physiological effects are regulated through intricate interactions or crosstalk among multiple phytohormones. MALDI‐MSI enables the simultaneous detection imaging of hormones. However, its application for tracing phytohormones is currently restricted by low abundance hormone in plant suboptimal matrix selection. 2,4‐Dihydroxy‐5‐nitrobenzoic acid (DHNBA) was reported as a new MALDI enhanced tissues. DHNBA demonstrates...
The transition from deep dormancy to seed germination is essential for the life cycle of plants, but how this process occurs in gymnosperm Chinese yew (Taxus chinensis var mairei), natural source anticancer drug paclitaxel, remains unclear. Herein, we analyse transcriptome, proteome, spatial metabolome, and lipidome present multi-omics profiles dormant germinating seeds. Our results show that abscisic acid gibberellic 12 homoeostasis closely associated with gene transcription protein...
Transgenic soybean is the commercial crop with largest cultivation area worldwide. During transgenic cultivation, exogenous genes may be transferred to wild relatives through gene flow, posing unpredictable ecological risks. Accordingly, an environmental risk assessment should focus on fitness changes and underlying mechanisms in hybrids between soybeans (Glycine soja). Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) was used for situ detection of protein...
The increased production and environmental release of graphene nanoparticles has raised concerns about its impact, but the effects on living organisms at metabolic level remain unknown. In this study, we used matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)-based untargeted metabolomics to investigate response juvenile earthworms (Eisenia fetida) exposure in soil tests for first time. Our results reveal that graphene-exposure significantly disturbs earthworm...
Amino acids (AAs), which are low-molecular-weight (low-MW) metabolites, serve as essential building blocks not only for protein synthesis but also maintaining the nitrogen balance in living systems. In situ detection and imaging of AAs crucial understanding more complex biological processes. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MSI) is a label-free spectrometric technique that enables simultaneous spatial distribution relative abundance different...
Michler's ethylketone (MEK, 4,4'-bis(diethylamino)benzophenone), commonly-known as an intermediate in the synthesis of dyes and pigments, was successfully screened optimized a novel matrix for enhancement lipid
In this study, polyacrylamide gel (PAAG) was successfully used as a new embedding medium to provide the more effective maintenance of biological tissues during sectioning process, enhancing tissue imaging metabolites via matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MSI). Herein, PAAG, agarose, gelatin, optimal cutting temperature compound (OCT), and ice media were embed rat liver Atlantic salmon (Salmo salar) eyeball samples. These embedded then sectioned into thin...
Herein, copper adhesive tape attached to the reverse side of a glass slide was developed as new method achieve protein in situ detection and imaging formalin fixed paraffin-embedded (FFPE) tissue section on non-conductive by MALDI-MSI. The use this led 223 ions being imaged from rat brain FFPE-tissue MALDI-MS, compared only 145 163 detected an ITO AnchorChip target plate, respectively. This has great potential become standard practice for MALDI-imaging sections slides.
Abstract Many environmental and industrial chemicals are reported to have androgenic or antiandrogenic activities. These may act as hormones the potential disrupt endocrine systems of wildlife humans. In this study, probabilistic neural network (PNN), support vector machine (SVM), learning quantization (LVQ), three types learning, were used develop binary classification models predict androgenicity directly from organic compounds' molecular structures which represented by only eleven...
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a powerful approach that has been widely used for in situ detection of various endogenous compounds tissues. However, there are still challenges with analysis proteins using MALDI-MSI due to the ion suppression effects small molecules tissue sections. Therefore, tissue-washing steps crucial protein MALDI remove these interfering molecules. Here, we successfully developed new method named...
Plant samples with irregular morphology are challenging for longitudinal tissue sectioning. This has restricted the ability to gain insight into some plants using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Herein, we develop a novel technique termed electromagnetic field-assisted frozen planarization (EMFAFTP). involves pair of adjustable electromagnets on both sides plant tissue. Under an optimized field strength, nondestructive and regularization is...