A5082265287- Neuroscience and Neuropharmacology Research
- Legume Nitrogen Fixing Symbiosis
- Neurogenesis and neuroplasticity mechanisms
- Zebrafish Biomedical Research Applications
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Neurobiology and Insect Physiology Research
- Nicotinic Acetylcholine Receptors Study
- Genetic and Environmental Crop Studies
- Neuroscience and Neural Engineering
- Protein Structure and Dynamics
- Ion channel regulation and function
- Protein Interaction Studies and Fluorescence Analysis
- Bone Tissue Engineering Materials
- Soybean genetics and cultivation
- Proteins in Food Systems
- Graphene and Nanomaterials Applications
- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- Neural dynamics and brain function
- DNA Repair Mechanisms
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Protein purification and stability
- Cardiac electrophysiology and arrhythmias
Sorbonne Université
2008-2024
Centre National de la Recherche Scientifique
2006-2024
Sorbonne University Abu Dhabi
2024
Polymères, Biopolymères, Surfaces
2023-2024
Institut National des Sciences Appliquées Rouen Normandie
2023-2024
Université de Rouen Normandie
2023-2024
Neurosciences Paris-Seine
2016-2023
Inserm
2013-2023
Institut de Biologie Paris-Seine
2018-2023
Université Paris-Seine
2020-2021
In Pisum sativum, the RAMOSUS genes RMS1, RMS2, and RMS5 regulate shoot branching via physiologically defined mobile signals. RMS1 is most likely a carotenoid cleavage enzyme acts with to control levels of an as yet unidentified inhibitor required for auxin inhibition branching. Our work provides molecular, genetic, physiological evidence that plays central role in shoot-to-root-to-shoot feedback system regulates pea. Indole-3-acetic acid (IAA) positively transcript level, potentially...
We have characterized a new gene, SWI1, involved in sister chromatid cohesion during both male and female meiosis Arabidopsis thaliana. A first allele, swi1.1, was obtained as T-DNA tagged mutant described previously abnormal exclusively meiosis. isolated swi1.2, which is defective for In swi1.2 meiosis, the classical steps of prophase were not observed, especially because homologs do synapse. Chromatid arms centromeres lost their stepwise manner before metaphase I, 20 chromatids instead...
The axon initial segment (AIS) is required for generating action potentials and maintaining neuronal polarity. Significant progress has been made in deciphering the basic building blocks composing AIS, but underlying mechanisms AIS formation remains unclear. scaffolding protein ankyrin-G master-organizer of AIS. Microtubules their interactors, particularly end-binding proteins (EBs), have emerged as potential key players formation. Here, we show that longest isoform (480AnkG) selectively...
A remarkable feature of early neuronal networks is their endogenous ability to generate spontaneous rhythmic electrical activity independently any external stimuli. In the mouse embryonic SC, this starts at an age ∼12 d and characterized by bursts action potentials recurring every 2–3 min. Although these have been extensively studied using extracellular recordings are known play important role in motoneuron (MN) maturation, mechanisms driving MN onset synaptogenesis still poorly understood....
The axon initial segment (AIS) is responsible for both the modulation of action potentials and maintenance neuronal polarity. Yet, molecular mechanisms controlling its assembly are incompletely understood. Our study in single electroporated motor neurons mouse embryos revealed that AnkyrinG (AnkG), AIS master organizer, undetectable bipolar migrating neurons, but already expressed at beginning axonogenesis E9.5 initially distributed homogeneously along entire growing axon. Then, from E11.5,...
Abstract While the role of cholinergic neurotransmission from motoneurons is well established during neuromuscular development, whether it regulates central nervous system development in spinal cord unclear. Zebrafish presents a powerful model to investigate how set up and evolves neural circuit formation. In this study, we carried out detailed spatiotemporal analysis embryonic larval zebrafish. 1-day-old embryos, show that express presynaptic genes including choline acetyltransferase (...
The ability of bovine serum albumin (BSA) to form condensates in crowded environments has been discovered only recently. Effects this condensed state on the secondary structure protein have already unraveled as some aging aspects, but pseudo-enzymatic behavior BSA never reported yet. This article investigates kinetic profile para-nitrophenol acetate hydrolysis by its with poly(ethylene) glycol (PEG) crowding agent. Furthermore, initial concentration was varied between 0.25 and 1 mM which...
Summary Modular organization of spinal neural circuits control dynamic regulation locomotion. However, it is unknown when or how the distinct microcircuits emerge during development. We carried out high-resolution calcium imaging activity driving first motor behavior in one day old zebrafish embryo. During this period, at least two waves neurogenesis occur to generate primary and secondary motoneurons. found that embryos display a single highly synchronized rhythmic neuronal circuit...
Rhizobial bacteria almost exclusively nodulate members of the families Fabaceae, Mimosaceae and Caesalpiniaceae, but are found on a single non-legume taxon, Parasponia (Ulmaceae). Based their host-range, nitrogen-fixing ability strain competition experiments, bacterial strains isolated from were thought to constitute separate lineage that would account for exceptional host affinity. This hypothesis was investigated by focusing four isolates representative morphological cultural types...
Abstract Virtually all oligodendrocyte precursors cells (OPCs) receive glutamatergic and/or GABAergic synapses that are lost upon their differentiation into oligodendrocytes in the postnatal and adult brain. Although OPCs generated at mid‐embryonic stages, several weeks before onset of myelination, it remains unknown when where first become susceptible to influence neuronal activity. In embryonic spinal cord, neuro‐epithelial pMN domain cease generating cholinergic motor neurons (MNs)...
Abstract Since the discovery of olfactory receptor (OR) genes, their expression in non-olfactory tissues have been reported rodents and humans. For example, mouse OR23 (mOR23) is expressed sperm muscle cells has proposed to play a role chemotaxis migration, respectively. In addition, mesencephalic dopaminergic neurons express various ORs, which respond corresponding ligands. As OR genes comprise largest multigene family G protein-coupled receptors vertebrates (over 400 human 1000 rodents),...
Liquid–liquid phase separation is an important process in living cells allowing for partitioning the cytoplasm and triggering biochemical reactions with higher reactivity than dilute case. The resulting droplets are typically composed of highly concentrated components such as proteins, which condensate due to particular intermolecular interactions. Macromolecular crowding one driving force responsible protein condensation can be leveraged vitro induce several types molecules. Herein, bovin...
Highlights- Surface functionalization of FAU via APTES or TESPA allowed to graft either amine carboxylate groups, mediate their covalent incorporation into a gelatin hydrogel.- Thermal properties the resulting composites were substantially improved compared with non-crosslinked conditions.- The FAU/gelatin found biocompatible respect macrophage-derived THP-1 cells.
SUMMARY In the developing central nervous system, electrical signaling is thought to rely exclusively on differentiating neurons as they acquire ability generate action potentials. Accordingly, neuroepithelial progenitors (NEPs) giving rise all and glial cells during development have been reported remain electrically passive. Here, we investigated physiological properties of NEPs in mouse spinal cord at onset spontaneous neural activity (SNA) initiating motor behavior embryos. Using...