M1 macrophage accumulation and excessive inflammation are commonly encountered issues in diabetic wounds can fail the healing process. Hence, hydrogel dressings with immunoregulatory capacity have great promise clinical practice of wound healing. However, current hydrogels always needed for complex interventions high-cost treatments, such as cytokines cell therapies. In this study, a novel glycyrrhizic acid (GA)-based hybrid dressing intrinsic properties is developed to promote rapid This...

Abstract Ocular injuries and their complications represent the most common causes of visual impairment. For ocular surgery, there is an unmet need for highly transparent bioadhesives with superior adhesion, biocompatibility, regenerative properties. Herein, a novel high‐transparent bioadhesive hydrogel composed gelatin methacryloyl (GelMA) dopamine methacrylamide (DMA) developed by in situ oxidative free‐radical polymerization. This overcomes fundamental weakness mussel‐inspired adhesive...

Abstract Lacrimal plug is an effective and widely therapeutic strategy to treat dry eye. However, almost all commercialized plugs are fixed in a certain design associated with many complications, such as spontaneous extrusion, epiphora, granuloma cannot be traced the long‐term. Herein, simple situ forming hydrogel developed tracer degradable lacrimal achieve best match irregular passages. In this strategy, methacrylate‐modified silk fibroin (SFMA) served network, self‐assembled indocyanine...

Abstract Background Drug combination that consists of distinctive agents is an attractive strategy to combat complex diseases and has been widely used clinically with improved therapeutic effects. However, the identification efficacious drug combinations remains a non-trivial challenging task due huge number possible among candidate drugs. As important factor, molecular context in which drugs exert their functions can provide crucial insights into mechanism underlying combinations. Results...

Aged adults are prone to both short- and long-term complications following sepsis due ineffective therapy. Phosphatidylserine (PS) is a membrane nutrient supplement known enhance cognition brain function, but its potential effects in treating not well-documented. Our study aimed explore the of PS improving outcomes sepsis-associated encephalopathy (SAE). Middle-aged mice were administered for two months induction by lipopolysaccharides. The results indicated significant increase survival...

Abstract Background Combination of different agents is widely used in clinic to combat complex diseases with improved therapy and reduced side effects. However, the identification effective drug combinations remains a challenging task due huge number possible among candidate drugs that makes it impractical screen putative combinations. Results In this work, we construct 'drug cocktail network' using all known extracted from Drug Database (DCDB), propose network-based approach investigate Our...

Abstract Accumulating evidence showed that cytokines are involved in the development of cancer. IL‐13 was to induce epithelial–mesenchymal transition and promote metastasis colorectal cancer, providing a promising therapeutic target for cancer patients. Interestingly, recent studies propofol, one most common intravenous anesthetic agent, may have antitumor function different type. However, impact propofol on induced remains unknown. Herein, we found can effectively suppress cell...

Sepsis-associated encephalopathy (SAE) is an intricated complication of sepsis that brings abnormal emotional and memory dysfunction increases patients’ mortality. Patients’ alterations function seen in SAE occur the hippocampus, primary brain region responsible for control, but underlying pathophysiological mechanisms remain unclear. In current study, we employed integrative analysis combining RNA-seq-based transcriptomics liquid chromatography/mass spectrometry (LC-MS)-based metabolomics...

Growing evidence suggests that cisplatin and other chemotherapeutic agents promote tumor metastasis while inhibiting growth, which is a critical issue for certain patients in clinical practices. However, the role of chemotherapeutics promoting molecular mechanism involved are unclear. Here, we investigated roles lung adenocarcinoma (LUAD). We demonstrated promoted epithelial-mesenchymal transition (EMT), cell motility, vitro vivo. The bioinformatic analysis biology approaches also indicated...

Aging is an intricate biological event that occurs in both vertebrates and invertebrates. During the aging process, brain, a vulnerable organ, undergoes structural functional alterations, resulting behavioral changes. The hippocampus has long been known to be critically associated with cognitive impairment, dementia, Alzheimer's disease during aging; however, underlying mechanisms remain largely unknown. In this study, we hypothesized altered metabolic gene expression profiles promote...

Abstract Background Signal transduction is an essential biological process involved in cell response to environment changes, by which extracellular signaling initiates intracellular signaling. Many computational methods have been generated mining signal networks with the increasing of high-throughput genomic and proteomic data. However, more effective means are still needed understand complex mechanisms pathways. Results We propose a new approach, namely CASCADE_SCAN, for from data based on...

Hydroxyapatite nanoparticles (HANPs) have been increasingly regarded and reported due to their potential anti-tumor ability. Previously, we found that the rod-like HANPs had good application for cutaneous melanoma (CMM). To satisfy actual requirements in repairing post-operative skin defects inhibiting CMM recurrence after tumorectomy, constructed a novel chitosan/alginate (CS/Alg) hydrogel containing aforementioned HANPs. The vitro cell experiments confirmed activated...

CD44+/CD24− cells have been associated with breast cancer stem/progenitor cell features. However, the status of this phenotype in normal, benign and malignant tissues has not studied, clinical correlation subpopulation is fully understood. The present study sought to identify these a series benign, explore their molecular subtypes carcinoma conventional pathological Double-staining immunohistochemistry (DIHC) CD44 CD24 was performed on 30 normal tissues, fibroadenomas (FA), 60 invasive...

Prediction tools for various intraoperative bleeding events remain scarce. We aim to develop machine learning-based models and identify the most important predictors by real-world data from electronic medical records (EMRs).An established database of surgical inpatients in Shanghai was utilized analysis. A total 51,173 were assessed eligibility. 48,543 obtained dataset patients divided into haemorrhage (N = 9728) without-haemorrhage 38,815) groups according their during procedure. Candidate...

Combination of different agents is widely used clinically to combat complex diseases with improved therapy and decreased side effects. It necessary understand the underlying mechanisms drug combinations. In this work, we proposed a network-based approach investigate Our results showed that in an effective combination tend have more similar therapeutic effects interaction partners `drug-cocktail network' than random networks. Based on our results, further developed statistical model termed as...

Dahuang Zhechong Pill (DHZCP) is a traditional Chinese medicine prescription used to treat many diseases especially chronic liver disease accompanied by promotion of vascular normalization. In this work, UPLC-Q-TOF-MS/MS analysis was applied identify the chemical components absorbed in blood. HIF-1α, VEGF, Ang2 and Tie2 related normalization were detected determine dynamic changes pharmacodynamic indicators. Then, spectrum-effect relationship between UHPLC fingerprint indicators evaluated...

The existence of mammary epithelial stem/progenitor cells has been demonstrated in MUC-1-/ESA+ subpopulations breast cells. However, knowledge about the expression and localization benign malignant lesions is unknown. Using a double-staining immunohistochemistry method, we investigated 10 normal tissues, 49 cases with fibrocystic disease, 40 fibroadenomas, 36 invasive ductal carcinomas cancer cell lines MCF-7 MDA-MB-468. In tissues were mainly found suprabasal layer, but under apical surface...

Global cerebral ischemia induced by cardiac arrest usually leads to poor neurological outcomes. Numerous studies have focused on ways prevent ischemic damage in the brain, however clinical therapies are still limited. Our previous revealed that delta opioid receptor (DOR) activation with [d-Ala2, d-Leu5] enkephalin (DADLE), a DOR agonist, not only significantly promotes neuronal survival day 3, but also improves spatial memory deficits days 5-9 after ischemia. However, mechanism underlying...

Abstract Thyroid cancer (TC), as the most common endocrine malignancy, has exhibited a significant increasing trend in incidence China and globally, with papillary thyroid (PTC) being pathological type. However, molecular mechanisms driving initiation progression of TC remains scarce, posing bottleneck problem for precise diagnosis treatment. Tumors, complex heterogeneous composite consisting tumor cells, immune stromal have long been considered to play key role occurrence malignant through...