Lipid emulsion has recently emerged as an effective agent for improving the cardiotoxicity of highly lipophilic drugs. However, its effect on induced by labetalol, a nonselective beta-blocker, remains unknown. In this study, we investigated effects lipid labetalol in rat cardiomyoblasts and tried to decipher underlying mechanisms. The labetalol-induced changes cell viability, expression Bax/Bcl-2, cleaved caspase-3, caspase-9, phosphorylation GSK-3β, Akt, PI3K were examined. inhibited...

Intravenous lipid emulsions (LEs) are effective in the treatment of toxicity associated with various drugs such as local anesthetics and other soluble agents.The goals this study were to examine effect LE on left ventricular hemodynamic variables systemic blood pressure an vivo rat model, determine cellular mechanism a particular focus nitric oxide.Two LEs (Intralipid  20% Lipofundin MCT/LCT 20%) or normal saline administered intravenously model following induction anesthesia by...

Mepivacaine is an aminoamide-linked local anesthetic with intermediate duration that intrinsically produces vasoconstriction both in vivo and vitro. The aims of this in-vitro study were to examine the direct effect mepivacaine isolated rat aortic rings determine associated cellular mechanism a particular focus on endothelium-derived vasodilators, which modulate vascular tone. In or without endothelium, cumulative concentration–response curves generated presence absence following antagonists:...

This study aimed to examine the endothelial dependence of vasodilation induced by phosphodiesterase inhibitor theophylline in isolated rat thoracic aortas and elucidate underlying mechanism, with emphasis on nitric oxide (NO). The effects various inhibitors denudation theophylline-induced vasodilation, effect NO donor sodium nitroprusside, cyclic guanosine monophosphate (cGMP) analog bromo-cGMP, β-agonist isoproterenol endothelium-denuded aorta were examined. nitroprusside cGMP formation...

This study aimed to examine the effect of lipid emulsion on vasodilation induced by a toxic dose amlodipine in isolated rat aorta and elucidate its mechanism, with particular focus nitric oxide. The effects endothelial denudation, NW-nitro-L-arginvine methyl ester (L-NAME), methylene blue, emulsion, linolenic acid amlodipine-induced cyclic guanosine monophosphate (cGMP) production were examined. Furthermore, amlodipine, PP2, either alone or combined, oxide synthase (eNOS), caveolin-1,...

Lipid emulsions are used as adjuvant drugs to alleviate intractable cardiovascular collapse induced by drug toxicity. We aimed examine the effect of lipid on labetalol-induced vasodilation and underlying mechanism in isolated rat aorta. studied effects endothelial denudation, N

It is well known that propofol protects myocardium against myocardial ischemia/reperfusion injury in the rat heart model. The aim of this study was to investigate whether provides a protective effect regional an vivo model after 48 h reperfusion.Rats were subjected 25 min left coronary artery occlusion followed by reperfusion. sham group received profopol without ischemic injury. control normal saline with intralipid A microcatheter advanced into ventricle and hemodynamic function evaluated....

The goal of this study was to examine the effect lipid emulsion on vasodilation induced by ATP-sensitive potassium (KATP) channels in isolated rat aortae and underlying mechanism. effects Intralipid, containing 100% long-chain fatty acids, Lipofundin MCT/LCT, 50% acids plus medium-chain levcromakalim endothelium-intact aorta with or without NW-nitro-L-arginine methyl ester (L-NAME) endothelium-denuded were examined. L-arginine, L-NAME, glibenclamide, alone combined, levcromakalim-induced...

Lee, Soo Hee MD; Park, Miyeong Ok, Seong-Ho Hwang, Yeran Kyeong-Eon Bae, Sung Il Yoon, Sangcheol Sohn, Ju-Tae MD Author Information

Amlodipine-induced toxicity has detrimental effects on cardiac cells. The aim of this study was to examine the effect lipid emulsion decreased H9c2 rat cardiomyoblast viability induced by amlodipine toxicity. amlodipine, emulsion, LY 294002, and glibenclamide, either alone or in combination, cell count, apoptosis, expression cleaved caspase-3 -8, Bax were examined. 294002 glibenclamide partially reversed emulsion-mediated attenuation count amlodipine. Amlodipine increased -8 expression, but...

In this in vitro study we examined the effects of diazepam on a phenylephrine-induced contraction rat aorta and determined associated cellular mechanism focusing endothelium-derived vasodilators. The concentration-response curves for phenylephrine potassium chloride were generated presence or absence diazepam. Phenylephrine from endothelium-intact rings pretreated independently with N(W)-nitro-L-arginine methyl ester, PK 11195, tetraethylammonium, indomethacin Diazepam (7 x 10(-7) M)...

Tramadol, a combination of R(-) and S(+) enantiomers, inhibits both the acetylcholine-mediated response muscarinic receptors muscarine-induced accumulation cyclic guanosine monophosphate. Our goals in this vitro study were to investigate effects tramadol on endothelium-dependent relaxation induced by acetylcholine, determine whether effect is stereoselective, elucidate associated cellular mechanism rat aorta. In endothelium-intact rings precontracted with phenylephrine or without naloxone,...

Spinal anesthesia and intrathecal clonidine are known to have hypnotic effects. We investigated the effect of spinal on requirement propofol for sedation.Sixty adult patients scheduled stripping ligation varicose veins under local or were enrolled in this study. Group 1 included given anesthesia, group 2 3 with 75 microg clonidine. Target-controlled infusion was started achieve a target concentration 1.5 microg/mL, mean bispectral index (BIS) min checked after an site (Ce) microg/mL reached....

Fentanyl was reported to inhibit the alpha(1)-adrenoceptor agonist-induced contraction. The goal of this in vitro study identify subtype primarily involved fentanyl-induced attenuation phenylephrine-induced contraction isolated endothelium-denuded rat aorta.Aortic rings were suspended order record isometric tension. Concentration-response curves for phenylephrine (10(-9) 10(-5) M) generated presence or absence one following drugs: fentanyl (3 x 10(-7), 10(-6), 3 x10(-6) M), 5-methylurapidil...

A phantom syndrome is a pain that occurs when part of the body, such as nose, tongue, breast, tooth, testis, penis, bladder, or anus, has been lost result an accident operation. Its frequency and etiology remain unclear [1]. Phantom bladder rare not reported previously in South Korea. This urinary phenomenon patients with cystectomies, undergoing hemodialysis, after spinal cord injury, forms lower abdominal during urination, frequent acute inconvenience full-bladder sensation, urination...

This study aims to examine the effect of linolenic acid on vasodilation or vasoconstriction induced by acetylcholine and bupivacaine in isolated rat aortae its underlying mechanism. The acetylcholine, calcium ionophore A23187, sodium nitroprusside, 8-bromoguanosine 3′,5′-cyclic monophosphate salt (bromo-cyclic guanosine [bromo-cGMP]) endothelium-intact endothelium-denuded was examined. Linolenic inhibited nitroprusside. However, this fatty increased bromo-cGMP-induced aortae....

In this study, we examined whether aortic contraction, induced by the alpha-2 adrenoceptor agonist dexmedetomidine, is involved in transactivation of epidermal growth factor receptor (EGFR) isolated endothelium-denuded rat aortas. Additionally, aimed to elucidate associated underlying cellular mechanisms. The effects inhibitor rauwolscine, EGFR tyrosine kinase AG1478, Src inhibitors PP1 and PP2, matrix metalloproteinase GM6001 on phosphorylation c-Jun NH2-terminal (JNK) dexmedetomidine...

Dexmedetomidine is widely used to induce sedation in the perioperative period. This study examined effect of hypothermia (33 and 25 °C) on dexmedetomidine-induced contraction an endothelium-intact aorta with or without nitric oxide synthase inhibitor NW-nitro-L-arginine methyl ester (L-NAME). In addition, induced by dexmedetomidine endothelium-denuded a calcium-free Krebs solution was examined. The effects protein kinase C (PKC), myosin light chain (MLC20) phosphorylation, Rho-kinase...

Epinephrine (EPI) or norepinephrine (NOR) is widely used to treat cardiovascular collapse during lipid emulsion (LE) resuscitation for drug toxicity. However, the effect of LE on vasoconstriction caused by EPI NOR remains unknown. The purpose this study was examine an (Intralipid) and in isolated rat aorta.

This study examined the effect of chloroquine on vasodilation induced by levcromakalim in isolated endothelium-denuded rat aortas and clarified underlying mechanisms. We effects chloroquine, hydroxychloroquine, lipid emulsion, reactive oxygen species (ROS) scavenger N-acetyl-ʟ-cysteine (NAC), KATP channel inhibitor glibenclamide levcromakaliminduced vasodilation. The NAC, membrane hyperpolarization ROS production were aortic vascular smooth muscle cells (VSMCs). Chloroquine inhibited...

This study aimed to examine the effect of lipid emulsion (LE) on vasoconstriction induced by dexmedetomidine (DMT) in isolated rat aorta and elucidate associated cellular mechanism. The LE, NW-nitro-L-arginine methyl ester (L-NAME), methyl-β-cyclodextrin (MβCD) DMT-induced contraction was examined. We investigated LE cyclic guanosine monophosphate (cGMP) formation DMT concentration. DMT,...